ROT3 Cytochrome P450 superfamily protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: ROT3
Official Full Name: Cytochrome P450 superfamily protein
Primary source: TAIR:AT4G36380
Locus tag: AT4G36380
See related: Araport:AT4G36380
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: AP22.10; AP22_10; CYP90C1; ROTUNDIFOLIA 3
Summary: Encodes a cytochrome P-450 gene that is involved in leaf blade expansion by controlling polar cell expansion in the leaf length direction. Member of the CYP90C CYP450 family. ROT3 was shown to be involved in brassinosteroid biosynthesis, most likely in the conversion step of typhasterol (TY) to castasterone (CS). As 6-deoxo-CS was unable to restore the phenotype of rot3-1, it has been postulated that ROT3 might be specifically involved in the conversion of TY to CS in the C6-oxidation pathway of brassinolide. Recently, CYP90C1 was shown to catalyse the C-23 hydroxylation of several brassinosteroids (the enzyme has a broad specificity for 22-hydroxylated substrates).
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Genomic context

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See ROT3 in Genome Data Viewer

Location:: chromosome: 4

Exon count:: 9

Sequence:: Chromosome: 4; NC_003075.7 (17187528..17192507, complement)

Chromosome 4 - NC_003075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003075.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Brassinosteroids Antagonize Jasmonate-Activated Plant Defense Responses through BRI1-EMS-SUPPRESSOR1 (BES1).
Title: Brassinosteroids Antagonize Jasmonate-Activated Plant Defense Responses through BRI1-EMS-SUPPRESSOR1 (BES1).
ROT3 modulates ethylene-induced petiole movement.
Title: Ethylene promotes hyponastic growth through interaction with ROTUNDIFOLIA3/CYP90C1 in Arabidopsis.
In vitro biochemical assays using insect cell-expressed proteins revealed that both CYP90C1 and CYP90D1 catalyze C-23 hydroxylation of various 22-hydroxylated Brassinosteroids with markedly different catalytic efficiencies.
Title: C-23 hydroxylation by Arabidopsis CYP90C1 and CYP90D1 reveals a novel shortcut in brassinosteroid biosynthesis.

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Pathways from PubChem

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General gene information

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Homology

Orthologs from OrthoDB

NM_001342408.1

Supported by ESTs:

NM_119801.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables 22alpha-hydroxysteroid 23-monooxygenase activity	IEA Inferred from Electronic Annotation more info
enables heme binding	IEA Inferred from Electronic Annotation more info
enables iron ion binding	IEA Inferred from Electronic Annotation more info
enables monooxygenase activity	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen	IDA Inferred from Direct Assay more info	PubMed
enables steroid hydroxylase activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in brassinosteroid biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within brassinosteroid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within brassinosteroid homeostasis	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within leaf development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within leaf morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within monopolar cell growth	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within petal development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within stamen development	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in chloroplast	ISM Inferred from Sequence Model more info

General protein information

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Preferred Names: Cytochrome P450 superfamily protein

NP_568002.1

ROTUNDIFOLIA 3 (ROT3); CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, E-class, group IV (InterPro:IPR002403), Cytochrome P450, conserved site (InterPro:IPR017972); BEST Arabidopsis thaliana protein match is: cytochrome P450, family 90, subfamily D, polypeptide 1 (TAIR:AT3G13730.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003075.7 Reference assembly

Range

17187528..17192507 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_119801.4 → NP_568002.1 Cytochrome P450 superfamily protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_568002.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O23242, Q058J8, Q0WS16, Q9M066

UniProtKB/TrEMBL

A0A178V4B0, A0A5S9Y002, A0A7G2F3W3

Conserved Domains (1) summary

PLN03141
Location:68 → 515

PLN03141; 3-epi-6-deoxocathasterone 23-monooxygenase; Provisional
NM_001342408.1 → NP_001328464.1 Cytochrome P450 superfamily protein [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A1P8B4R4