Wasl WASP like actin nucleation promoting factor [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Waslprovided by MGI
Official Full Name: WASP like actin nucleation promoting factorprovided by MGI
Primary source: MGI:MGI:1920428
See related: Ensembl:ENSMUSG00000029684 AllianceGenome:MGI:1920428
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: N-WASP; 2900021I12Rik; 3110031I02Rik
Summary: Predicted to enable cytoskeletal protein binding activity and identical protein binding activity. Involved in dendritic spine morphogenesis and regulation of postsynapse organization. Acts upstream of or within several processes, including actin cytoskeleton organization; response to bacterium; and spindle localization. Located in actin cap and lamellipodium. Is active in glutamatergic synapse. Is expressed in brain; genitourinary system; liver; and spleen. Used to study atopic dermatitis. Orthologous to human WASL (WASP like actin nucleation promoting factor). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in bladder adult (RPKM 16.7), adrenal adult (RPKM 13.4) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 6 A3.1; 6 11.28 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	6	NC_000072.7 (24613809..24665185, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	6	NC_000072.6 (24613810..24664995, complement)

Chromosome 6 - NC_000072.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Neural Wiskott-Aldrich syndrome protein (N-WASP) promotes distant metastasis in pancreatic ductal adenocarcinoma via activation of LOXL2.
Title: Neural Wiskott-Aldrich syndrome protein (N-WASP) promotes distant metastasis in pancreatic ductal adenocarcinoma via activation of LOXL2.
N-WASP-dependent branched actin polymerization attenuates B-cell receptor signaling by increasing the molecular density of receptor clusters.
Title: N-WASP-dependent branched actin polymerization attenuates B-cell receptor signaling by increasing the molecular density of receptor clusters.
nWASP Inhibition Increases Wound Healing via TrKb/PLCgamma Signalling.
Title: nWASP Inhibition Increases Wound Healing via TrKb/PLCγ Signalling.
Loss of Wasl improves pancreatic cancer outcome.
Title: Loss of Wasl improves pancreatic cancer outcome.
N-Wasp Regulates Oligodendrocyte Myelination.
Title: N-Wasp Regulates Oligodendrocyte Myelination.
N-WASP regulates the mobility of the B cell receptor.N-WASP regulates the diffusivity of CD19, a stimulatory co-receptor, but not that of FcgammaRIIB.
Title: WASP family proteins regulate the mobility of the B cell receptor during signaling activation.
ta indicated that N-WASP deficiency in HGFs increases the production of inflammatory cytokine and is regulated via NF-kappaB and MAPK signaling pathways
Title: N-WASP knockdown upregulates inflammatory cytokines expression in human gingival fibroblasts.
N-WASP has an early tumour suppressive role in APC-induced intestinal carcinogenesis.
Title: Loss of N-WASP drives early progression in an Apc model of intestinal tumourigenesis.
conditional N-WASP knockout in keratinocytes leads to compromised skin barrier, higher infiltration of immune cells and hyperproliferation of keratinocytes due to increased production of cytokines highlighting the importance of N-WASP in maintaining the skin homeostasis.
Title: Conditional knock out of N-WASP in keratinocytes causes skin barrier defects and atopic dermatitis-like inflammation.
N-WASP regulates cortical neuron migration mainly through its polyPro and VCA domains.
Title: [N-WASP regulates cortical neuron migration through its polyPro and VCA domains].

Submit: New GeneRIF Correction See all GeneRIFs (48)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (6) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables actin binding	IEA Inferred from Electronic Annotation more info
enables cytoskeletal protein binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin filament polymerization	ISO Inferred from Sequence Orthology more info
involved_in actin filament polymerization	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within actin nucleation	TAS Traceable Author Statement more info	PubMed
involved_in cell division	IEA Inferred from Electronic Annotation more info
NOT acts_upstream_of_or_within cell projection assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dendritic spine morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in membrane invagination	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of lymphocyte migration	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of lymphocyte migration	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of membrane tubulation	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of membrane tubulation	ISO Inferred from Sequence Orthology more info
involved_in plasma membrane tubulation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of chemotaxis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of clathrin-dependent endocytosis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of clathrin-dependent endocytosis	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of filopodium assembly	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of filopodium assembly	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	ISS Inferred from Sequence or Structural Similarity more info
involved_in postsynapse organization	ISO Inferred from Sequence Orthology more info
involved_in postsynaptic actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein-containing complex localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell projection assembly	ISO Inferred from Sequence Orthology more info
involved_in regulation of postsynapse organization	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of postsynapse organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within spindle localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vesicle budding from membrane	ISO Inferred from Sequence Orthology more info
involved_in vesicle budding from membrane	ISS Inferred from Sequence or Structural Similarity more info
involved_in vesicle organization	ISO Inferred from Sequence Orthology more info
involved_in vesicle organization	ISS Inferred from Sequence or Structural Similarity more info
involved_in vesicle transport along actin filament	ISO Inferred from Sequence Orthology more info
involved_in vesicle transport along actin filament	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in Golgi membrane	ISO Inferred from Sequence Orthology more info
located_in actin cap	IDA Inferred from Direct Assay more info	PubMed
located_in cell leading edge	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IC Inferred by Curator more info	PubMed
located_in cytoplasmic vesicle	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic vesicle	ISS Inferred from Sequence or Structural Similarity more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	TAS Traceable Author Statement more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in lamellipodium	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in postsynapse	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: actin nucleation-promoting factor WASL

Names: Wiskott-Aldrich syndrome-like; neural Wiskott-Aldrich syndrome protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001167745.1 → NP_001161217.1 actin nucleation-promoting factor WASL isoform 2

See identical proteins and their annotated locations for NP_001161217.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1.

Source sequence(s)

AK014104, BC058642

Consensus CDS

CCDS51727.1

UniProtKB/TrEMBL

Q9CXQ9

Related

ENSMUSP00000043968.8, ENSMUST00000041737.8

Conserved Domains (1) summary

pfam00568
Location:28 → 135

WH1; WH1 domain
NM_028459.2 → NP_082735.2 actin nucleation-promoting factor WASL isoform 1

See identical proteins and their annotated locations for NP_082735.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AJ318416, AK143969, BC058642, CX567238

Consensus CDS

CCDS19945.1

UniProtKB/Swiss-Prot

Q91YD9

UniProtKB/TrEMBL

Q3TXX8, Q7TPN5

Related

ENSMUSP00000031695.9, ENSMUST00000031695.15

Conserved Domains (4) summary

pfam00568
Location:28 → 135

WH1; WH1 domain

pfam00786
Location:199 → 256

PBD; P21-Rho-binding domain

cd22074
Location:398 → 424

WH2_N-WASP_r1; first tandem Wiskott Aldrich syndrome homology region 2 (WH2 motif) repeat found in human Neural Wiskott-Aldrich syndrome protein (N-WASP) and related domains

cd22075
Location:425 → 449

WH2_hN-WASP_r2_like; second tandem Wiskott Aldrich syndrome homology region 2 (WH2 motif) repeat found in human Neural Wiskott-Aldrich syndrome protein (N-WASP) and related domains

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000072.7 Reference GRCm39 C57BL/6J

Range

24613809..24665185 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036152346.1 → XP_036008239.1 actin nucleation-promoting factor WASL isoform X1

Conserved Domains (2) summary

pfam00568
Location:28 → 135

WH1; WH1 domain

pfam00786
Location:199 → 256

PBD; P21-Rho-binding domain