CPL1 C-terminal domain phosphatase-like 1 [Arabidopsis thaliana (thale cress)] - Gene

Summary

Go to the top of the page Help

Official Symbol: CPL1
Official Full Name: C-terminal domain phosphatase-like 1
Primary source: TAIR:AT4G21670
Locus tag: AT4G21670
See related: Araport:AT4G21670
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ATCPL1; ATCPL2; C-terminal domain phosphatase-like 1; C-TERMINAL DOMAIN PHOSPHATASE-LIKE 1; F17L22.130; F17L22_130; FIERY 2; FRY2; RCF2; regulators of C-REPEAT BINDING FACTOR (CBF) gene expression 2; SHI4; SHINY 4
Summary: encodes a a novel transcriptional repressor harboring two double-stranded RNA-binding domains and a region homologous to the catalytic domain of RNA polymerase II C-terminal domain phosphatases found in yeast and in animals that regulate gene transcription. Protein exhibits innate phosphatase activity in vitro. Mutants exhibit hyperresponsiveness to ABA, cold, and NaCl.
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See CPL1 in Genome Data Viewer

Location:: chromosome: 4

Exon count:: 17

Sequence:: Chromosome: 4; NC_003075.7 (11510989..11517396)

Chromosome 4 - NC_003075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_003075.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

C-TERMINAL DOMAIN PHOSPHATASE-LIKE 1 promotes flowering with TAF15b by repressing the floral repressor gene FLOWERING LOCUS C.
Title: C-TERMINAL DOMAIN PHOSPHATASE-LIKE 1 promotes flowering with TAF15b by repressing the floral repressor gene FLOWERING LOCUS C.
C-terminal domain phosphatase-like 1 (CPL1) is involved in floral transition in Arabidopsis.
Title: C-terminal domain phosphatase-like 1 (CPL1) is involved in floral transition in Arabidopsis.
These results define a new role for CPL1 as a pathogen and pest susceptibility gene. Global transcriptome analysis and oxidative stress assays showed these cpl1 mutants have increased tolerance to oxidative stress.
Title: The Arabidopsis RNA Polymerase II Carboxyl Terminal Domain (CTD) Phosphatase-Like1 (CPL1) is a biotic stress susceptibility gene.
These results indicate that the coding region of Luciferase is responsible for the cpl1-specific Luciferase overexpression uncoupled with the expression of the endogenous counterpart.
Title: The coding sequence of firefly luciferase reporter gene affects specific hyperexpression in Arabidopsis thaliana cpl1 mutant.
A subset of alternatively spliced transcripts and 5'-extended mRNAs with Nonsense-mediated decay (NMD)-eliciting features accumulated in the fry2-1 mutant, cycloheximide-treated wild type, and upf3 mutant plants, indicating that FRY2 is essential for the degradation of these NMD transcripts.
Title: The RNA Polymerase II C-Terminal Domain Phosphatase-Like Protein FIERY2/CPL1 Interacts with eIF4AIII and Is Essential for Nonsense-Mediated mRNA Decay in Arabidopsis.
RCF2 interacts with the NAC transcription factor NAC019 and that RCF2 dephosphorylates NAC019
Title: The protein phosphatase RCF2 and its interacting partner NAC019 are critical for heat stress-responsive gene regulation and thermotolerance in Arabidopsis.
These results suggest that CPL1 regulates DNA methylation via a miRNA-dependent pathway.
Title: Arabidopsis C-terminal domain phosphatase-like 1 functions in miRNA accumulation and DNA methylation.
The root-to-shoot translocation of cadmium (Cd) in cpl1-2 is mediated by yet uncharacterized Cd transport mechanisms.
Title: Function of Arabidopsis CPL1 in cadmium responses.
FRY2/CPL1 may also recruit specific factors to regulate the co-transcriptional processing of certain transcripts to deal with environmental challenges.
Title: A KH-domain RNA-binding protein interacts with FIERY2/CTD phosphatase-like 1 and splicing factors and is important for pre-mRNA splicing in Arabidopsis.
we report the identification of a K-homology domain-containing protein named SHINY1 (SHI1) that interacts with CPL1 to modulate gene expression.
Title: The arabidopsis RNA binding protein with K homology motifs, SHINY1, interacts with the C-terminal domain phosphatase-like 1 (CPL1) to repress stress-inducible gene expression.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

NM_118287.5

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables RNA polymerase II CTD heptapeptide repeat phosphatase activity	IEA Inferred from Electronic Annotation more info
enables myosin phosphatase activity	IEA Inferred from Electronic Annotation more info
enables phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphoprotein phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within abscisic acid-activated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to salt stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to wounding	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISM Inferred from Sequence Model more info

General protein information

Go to the top of the page Help

Preferred Names: C-terminal domain phosphatase-like 1

NP_193898.3

C-terminal domain phosphatase-like 1 (CPL1); FUNCTIONS IN: double-stranded RNA binding, phosphatase activity, nucleotide phosphatase activity; INVOLVED IN: abscisic acid mediated signaling pathway, negative regulation of transcription, response to abiotic stimulus; LOCATED IN: intracellular; CONTAINS InterPro DOMAIN/s: Double-stranded RNA-binding (InterPro:IPR001159), NLI interacting factor (InterPro:IPR004274); BEST Arabidopsis thaliana protein match is: carboxyl-terminal domain (ctd) phosphatase-like 2 (TAIR:AT5G01270.2); Has 330 Blast hits to 317 proteins in 105 species: Archae - 0; Bacteria - 31; Metazoa - 57; Fungi - 81; Plants - 112; Viruses - 1; Other Eukaryotes - 48 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003075.7 Reference assembly

Range

11510989..11517396

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_118287.5 → NP_193898.3 C-terminal domain phosphatase-like 1 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_193898.3

Status: REVIEWED

UniProtKB/Swiss-Prot

Q0WNZ7, Q56WT8, Q5YDB6, Q9SVT0

UniProtKB/TrEMBL

A0A178V2E7

Conserved Domains (2) summary

smart00358
Location:857 → 922

DSRM; Double-stranded RNA binding motif

cl21460
Location:272 → 366

HAD_like; Haloacid Dehalogenase-like Hydrolases