DRB2 dsRNA-binding protein 2 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: DRB2
Official Full Name: dsRNA-binding protein 2
Primary source: TAIR:AT2G28380
Locus tag: AT2G28380
See related: Araport:AT2G28380
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: dsRNA-binding protein 2; T1B3.10; T1B3_10
Summary: Encodes a cytoplasmic dsRNA-binding protein DRB2. A maternally expressed imprinted gene. DRB2 and DRB4 have an antagonistic impact on polymerase IV-dependent siRNA levels.
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Genomic context

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See DRB2 in Genome Data Viewer

Location:: chromosome: 2

Exon count:: 3

Sequence:: Chromosome: 2; NC_003071.7 (12133732..12136241, complement)

Chromosome 2 - NC_003071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003071.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Immunocapture of dsRNA-bound proteins provides insight into Tobacco rattle virus replication complexes and reveals Arabidopsis DRB2 to be a wide-spectrum antiviral effector.
Title: Immunocapture of dsRNA-bound proteins provides insight into Tobacco rattle virus replication complexes and reveals Arabidopsis DRB2 to be a wide-spectrum antiviral effector.
DRB2-associated translational inhibition appears to be less ubiquitous and specifically aimed toward responses against abiotic or biotic stimuli.
Title: MicroRNA Regulatory Mechanisms Play Different Roles in Arabidopsis.
DRB2 is part of a repressive epigenetic regulator complex involved in a negative feedback loop, adjusting epigenetic state to transcription level at transposable element loci.
Title: Parallel action of AtDRB2 and RdDM in the control of transposable element expression.
Unlike DRB2, DRB3 and DRB5 are not involved in the double stranded RNA processing stages of the microRNA (miRNA) biogenesis pathway, but are required to mediate RNA silencing of target genes of DRB2-associated miRNAs.
Title: DRB2, DRB3 and DRB5 function in a non-canonical microRNA pathway in Arabidopsis thaliana.
Examination of different single and multiple drb mutants revealed a clear association between the loss of DRB2 activity and altered accumulation for both the elevated and reduced miRNA classes.
Title: DRB2 is required for microRNA biogenesis in Arabidopsis thaliana.
show that the double-stranded RNA binding proteins DRB2 and DRB4 are required for proper accumulation of p4-siRNAs.
Title: Double-stranded RNA binding proteins DRB2 and DRB4 have an antagonistic impact on polymerase IV-dependent siRNA levels in Arabidopsis.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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NM_128398.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables double-stranded RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within miRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISM Inferred from Sequence Model more info

General protein information

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Preferred Names: dsRNA-binding protein 2

NP_565672.1

dsRNA-binding protein 2 (DRB2); CONTAINS InterPro DOMAIN/s: Double-stranded RNA-binding (InterPro:IPR001159), Double-stranded RNA-binding-like (InterPro:IPR014720); BEST Arabidopsis thaliana protein match is: dsRNA-binding protein 5 (TAIR:AT5G41070.1); Has 1035 Blast hits to 903 proteins in 271 species: Archae - 2; Bacteria - 346; Metazoa - 166; Fungi - 72; Plants - 365; Viruses - 9; Other Eukaryotes - 75 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003071.7 Reference assembly

Range

12133732..12136241 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_128398.4 → NP_565672.1 dsRNA-binding protein 2 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_565672.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9SKN2

UniProtKB/TrEMBL

A0A5S9X2A2

Conserved Domains (2) summary

cd00048
Location:2 → 68

DSRM; Double-stranded RNA binding motif. Binding is not sequence specific but is highly specific for double stranded RNA. Found in a variety of proteins including dsRNA dependent protein kinase PKR, RNA helicases, Drosophila staufen protein, E. coli RNase III, ...

pfam00035
Location:88 → 153

dsrm; Double-stranded RNA binding motif