FAD2 fatty acid desaturase 2 [Arabidopsis thaliana (thale cress)] - Gene

Summary

Go to the top of the page Help

Official Symbol: FAD2
Official Full Name: fatty acid desaturase 2
Primary source: TAIR:AT3G12120
Locus tag: AT3G12120
See related: Araport:AT3G12120
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: AtFAD2; DELTA-12 DESATURASE; fatty acid desaturase 2
Summary: Major enzyme responsible for the synthesis of 18:2 fatty acids in the endoplasmic reticulum. Contains His-rich motifs, which contribute to the interaction with the electron donor cytochrome b5. Mutations in this gene suppress the low temperature-induced phenotype of Arabidopsis tocopherol-deficient mutant vte2.
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See FAD2 in Genome Data Viewer

Location:: chromosome: 3

Exon count:: 3

Sequence:: Chromosome: 3; NC_003074.8 (3860251..3863250, complement)

Chromosome 3 - NC_003074.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Association of the Arabidopsis oleoyl Delta12-desaturase FAD2 with pre-cis-Golgi stacks at endoplasmic reticulum-Golgi-exit sites.
Title: Association of the Arabidopsis oleoyl Δ12-desaturase FAD2 with pre-cis-Golgi stacks at endoplasmic reticulum-Golgi-exit sites.
Results suggest that membrane lipid polyunsaturation mediated by fatty acid desaturase 2 (FAD2) is involved in endoplasmic reticulum (ER) stress tolerance in Arabidopsis
Title: Membrane lipid polyunsaturation mediated by FATTY ACID DESATURASE 2 (FAD2) is involved in endoplasmic reticulum stress tolerance in Arabidopsis thaliana.
Based on the authors' findings in flax, carinata, camelina, and Arabidopsis in this study, they suggest that co-suppression of FAD2 occurs widely in plants and therefore may present a common barrier to improving 18:2 content via bioengineering FAD2 expression.
Title: Strong co-suppression impedes an increase in polyunsaturated fatty acids in seeds overexpressing FAD2.
Data suggest that omega-6 desaturation is largely controlled by cis-acting sequence variants in the fatty acid desaturase 2 (FAD2) 5' untranslated region intron that determine the expression level of the gene.
Title: Genome Wide Analysis of Fatty Acid Desaturation and Its Response to Temperature.
ALA10 interacts with FATTY ACID DESATURASE2 (FAD2), and modification of ALA10 expression affects phosphatidylcholine (PC) fatty acyl desaturation by disturbing the balance between FAD2 and FAD3 activities.
Title: ALA10, a Phospholipid Flippase, Controls FAD2/FAD3 Desaturation of Phosphatidylcholine in the ER and Affects Chloroplast Lipid Composition in Arabidopsis thaliana.
FAD2 and FATB may have influenced the adaptive evolution of seed oil composition to optimize seed oil melting point relative to germination temperatures
Title: Genome-Wide Association Study in Arabidopsis thaliana of Natural Variation in Seed Oil Melting Point: A Widespread Adaptive Trait in Plants.
FAD2 and FAD3 desaturases form heterodimers that facilitate metabolic channel.
Title: FAD2 and FAD3 desaturases form heterodimers that facilitate metabolic channeling in vivo.
Results suggest that FAD2 mediated high-level vacuolar and plasma membrane fatty acid desaturation is essential for the proper function of membrane attached Na+/H+ exchangers.
Title: Arabidopsis fatty acid desaturase FAD2 is required for salt tolerance during seed germination and early seedling growth.
The fatty acid composition of membrane lipids influences cell growth and mitochondrial respiration, in particular the expression and capacity of alternative oxidase, under cold stress in fad2 knockout and FDAD3 overexpressing cell lines
Title: Alternative oxidase involvement in cold stress response of Arabidopsis thaliana fad2 and FAD3+ cell suspensions altered in membrane lipid composition.

Submit: New GeneRIF Correction

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

Orthologs from OrthoDB

NM_001337975.1

Supported by ESTs:

NM_112047.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables acyl-lipid Delta(12)-acetylenase	IGI Inferred from Genetic Interaction more info	PubMed
enables acyl-lipid omega-6 desaturase (cytochrome b5) activity	IEA Inferred from Electronic Annotation more info
enables delta12-fatty-acid desaturase activity	IEA Inferred from Electronic Annotation more info
enables omega-6 fatty acid desaturase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in unsaturated fatty acid biosynthetic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	NAS Non-traceable Author Statement more info
located_in nucleus	ISM Inferred from Sequence Model more info

General protein information

Go to the top of the page Help

Preferred Names: fatty acid desaturase 2

NP_001319529.1

fatty acid desaturase 2 (FAD2); FUNCTIONS IN: omega-6 fatty acid desaturase activity, delta12-fatty acid dehydrogenase activity; INVOLVED IN: oxidation reduction, lipid metabolic process; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF3474 (InterPro:IPR021863), Fatty acid desaturase, type 1 (InterPro:IPR005804); BEST Arabidopsis thaliana protein match is: fatty acid desaturase 3 (TAIR:AT2G29980.1); Has 2431 Blast hits to 2431 proteins in 654 species: Archae - 0; Bacteria - 821; Metazoa - 31; Fungi - 266; Plants - 965; Viruses - 0; Other Eukaryotes - 348 (source: NCBI BLink).

NP_187819.1

fatty acid desaturase 2 (FAD2); FUNCTIONS IN: omega-6 fatty acid desaturase activity, delta12-fatty acid dehydrogenase activity; INVOLVED IN: oxidation reduction, lipid metabolic process; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF3474 (InterPro:IPR021863), Fatty acid desaturase, type 1 (InterPro:IPR005804); BEST Arabidopsis thaliana protein match is: fatty acid desaturase 3 (TAIR:AT2G29980.1); Has 2431 Blast hits to 2431 proteins in 654 species: Archae - 0; Bacteria - 821; Metazoa - 31; Fungi - 266; Plants - 965; Viruses - 0; Other Eukaryotes - 348 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003074.8 Reference assembly

Range

3860251..3863250 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_112047.4 → NP_187819.1 fatty acid desaturase 2 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_187819.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P46313, Q19MZ0, Q8LFZ8

UniProtKB/TrEMBL

A0A178VIK7, A0A5S9XBB7, A0A654F696

Conserved Domains (1) summary

PLN02505
Location:1 → 383

PLN02505; omega-6 fatty acid desaturase
NM_001337975.1 → NP_001319529.1 fatty acid desaturase 2 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/Swiss-Prot

P46313, Q19MZ0, Q8LFZ8

UniProtKB/TrEMBL

A0A178VIK7, A0A5S9XBB7, A0A654F696

Conserved Domains (1) summary

PLN02505
Location:1 → 383

PLN02505; omega-6 fatty acid desaturase