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    REF4 reduced epidermal fluorescence 4 [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 819423, updated on 18-Sep-2024

    Summary

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    Official Symbol
    REF4
    Official Full Name
    reduced epidermal fluorescence 4
    Primary source
    TAIR:AT2G48110
    Locus tag
    AT2G48110
    See related
    Araport:AT2G48110
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    F11L15.1; MED33B; REDUCED EPIDERMAL FLUORESCENCE 4; T9J23.25
    Summary
    Encodes a novel protein of unknown function with homologs in non-seed plants. Sequence analysis predicts membrane spanning domains and a putative protein-protein interaction domain. Semi-dominant mutations display defects in phenylpropanoid accumulation suggesting a role in phenylpropanoid metabolism. It has been shown to physically associate with the conserved transcriptional coregulatory complex, Mediator, and is involved in the regulation of phenylpropanoid homeostasis. Acts redundantly with REF4/MED5b (At3g23590). Required for expression of some dark-upregulated genes.
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    Genomic context

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    See REF4 in Genome Data Viewer
    Location:
    chromosome: 2
    Exon count:
    14
    Sequence:
    Chromosome: 2; NC_003071.7 (19673293..19679711)

    Chromosome 2 - NC_003071.7Genomic Context describing neighboring genes Neighboring gene uncharacterized protein Neighboring gene Exonuclease family protein Neighboring gene pale cress protein (PAC) Neighboring gene ribonuclease III family protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003071.7

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Semidominant mutations in reduced epidermal fluorescence 4 reduce phenylpropanoid content in Arabidopsis. Stout J, et al. Genetics, 2008 Apr. PMID 18430946, Free PMC Article
    2. Mutation of Mediator subunit CDK8 counteracts the stunted growth and salicylic acid hyperaccumulation phenotypes of an Arabidopsis MED5 mutant. Mao X, et al. New Phytol, 2019 Jul. PMID 30756399
    3. REF4 and RFR1, subunits of the transcriptional coregulatory complex mediator, are required for phenylpropanoid homeostasis in Arabidopsis. Bonawitz ND, et al. J Biol Chem, 2012 Feb 17. PMID 22167189, Free PMC Article
    4. Purification of a plant mediator from Arabidopsis thaliana identifies PFT1 as the Med25 subunit. Bäckström S, et al. Mol Cell, 2007 Jun 8. PMID 17560376
    5. Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana. Lin X, et al. Nature, 1999 Dec 16. PMID 10617197

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Study reveals genetic interactions between Mediator tail and kinase module subunits and enhances our understanding of dwarfing in phenylpropanoid pathway mutants. [MED5b]
      Title: Mutation of Mediator subunit CDK8 counteracts the stunted growth and salicylic acid hyperaccumulation phenotypes of an Arabidopsis MED5 mutant.
    2. disruption of both REF4 and its paralog, REF4-RELATED 1 (RFR1), results in enhanced expression of multiple phenylpropanoid biosynthetic genes
      Title: REF4 and RFR1, subunits of the transcriptional coregulatory complex mediator, are required for phenylpropanoid homeostasis in Arabidopsis.
    3. The REF4 locus (At2g48110) was cloned by a combined map- and sequencing-based approach and was found to encode a large integral membrane protein that is unique to plants.
      Title: Semidominant mutations in reduced epidermal fluorescence 4 reduce phenylpropanoid content in Arabidopsis.
    Submit: New GeneRIF Correction

    General gene information

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    Markers

    NM_130378.5

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables molecular_function ND
    No biological Data available
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within phenylpropanoid metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within regulation of phenylpropanoid metabolic process IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of mediator complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane TAS
    Traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    reduced epidermal fluorescence 4
    NP_566125.4
    • REDUCED EPIDERMAL FLUORESCENCE 4 (REF4); BEST Arabidopsis thaliana protein match is: REF4-related 1 (TAIR:AT3G23590.1); Has 140 Blast hits to 138 proteins in 18 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 140; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003071.7 Reference assembly

      Range
      19673293..19679711
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_130378.5 → NP_566125.4  reduced epidermal fluorescence 4 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_566125.4

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      F4IN69, Q39169, Q9ZU78
      UniProtKB/TrEMBL
      A0A654F4C0
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    F4IN69.1

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