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    Dhfr dihydrofolate reductase [ Mus musculus (house mouse) ]

    Gene ID: 13361, updated on 4-Jan-2025

    Summary

    Official Symbol
    Dhfrprovided by MGI
    Official Full Name
    dihydrofolate reductaseprovided by MGI
    Primary source
    MGI:MGI:94890
    See related
    Ensembl:ENSMUSG00000021707 AllianceGenome:MGI:94890
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    8430436I03Rik
    Summary
    Enables dihydrofolate reductase activity. Involved in several processes, including positive regulation of nitric-oxide synthase activity; tetrahydrobiopterin biosynthetic process; and tetrahydrofolate interconversion. Located in mitochondrion. Is active in nucleus. Is expressed in several structures, including genitourinary system; hemolymphoid system; integumental system; limb; and nervous system. Human ortholog(s) of this gene implicated in acute lymphoblastic leukemia. Orthologous to several human genes including DHFR (dihydrofolate reductase). [provided by Alliance of Genome Resources, Jan 2025]
    Expression
    Broad expression in liver E14 (RPKM 9.4), liver E14.5 (RPKM 7.0) and 22 other tissues See more
    Orthologs
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    Genomic context

    See Dhfr in Genome Data Viewer
    Location:
    13 C3; 13 47.64 cM
    Exon count:
    6
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 13 NC_000079.7 (92491291..92525561)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 13 NC_000079.6 (92354783..92389053)

    Chromosome 13 - NC_000079.7Genomic Context describing neighboring genes Neighboring gene RAS protein-specific guanine nucleotide-releasing factor 2 Neighboring gene predicted gene, 46435 Neighboring gene STARR-seq mESC enhancer starr_35260 Neighboring gene STARR-seq mESC enhancer starr_35261 Neighboring gene STARR-seq mESC enhancer starr_35262 Neighboring gene mutS homolog 3 Neighboring gene STARR-positive B cell enhancer mm9_chr13:93090941-93091242 Neighboring gene predicted gene, 20379 Neighboring gene STARR-positive B cell enhancer ABC_E8539 Neighboring gene STARR-positive B cell enhancer ABC_E2416 Neighboring gene 60S ribosomal protein L32 pseudogene Neighboring gene ankyrin repeat domain 34B Neighboring gene STARR-seq mESC enhancer starr_35266 Neighboring gene family with sequence similarity 151, member B

    Genomic regions, transcripts, and products

    Expression

    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    Variation

    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Chemically induced (ENU) (1) 
    • Endonuclease-mediated (2)  1 citation
    • Targeted (4) 

    Pathways from PubChem

    Interactions

    General gene information

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables NADP binding  
    enables NADP binding  
    enables NADP binding  
    enables NADPH binding  
    enables dihydrofolate reductase activity  
    enables dihydrofolate reductase activity PubMed 
    enables dihydrofolate reductase activity  
    enables dihydrofolate reductase activity  
    enables dihydrofolic acid binding  
    enables dihydrofolic acid binding  
    enables folic acid binding  
    enables mRNA binding  
    enables mRNA binding  
    enables mRNA regulatory element binding translation repressor activity  
    enables sequence-specific mRNA binding  
    Process Evidence Code Pubs
    involved_in axon regeneration  
    involved_in axon regeneration  
    involved_in dihydrofolate metabolic process  
    involved_in dihydrofolate metabolic process  
    involved_in dihydrofolate metabolic process  
    involved_in folic acid metabolic process  
    involved_in folic acid metabolic process  
    involved_in folic acid metabolic process  
    involved_in positive regulation of nitric-oxide synthase activity PubMed 
    involved_in regulation of removal of superoxide radicals PubMed 
    involved_in response to methotrexate  
    involved_in response to nicotine  
    involved_in tetrahydrobiopterin biosynthetic process PubMed 
    involved_in tetrahydrofolate biosynthetic process  
    involved_in tetrahydrofolate biosynthetic process  
    involved_in tetrahydrofolate biosynthetic process  
    involved_in tetrahydrofolate interconversion PubMed 
    involved_in tetrahydrofolate metabolic process  
    Component Evidence Code Pubs
    located_in cytoplasm PubMed 
    located_in cytoplasm  
    located_in cytosol  
    located_in mitochondrial inner membrane  
    located_in mitochondrial matrix  
    is_active_in mitochondrion  
    located_in mitochondrion PubMed 
    located_in mitochondrion  
    is_active_in nucleus PubMed 

    General protein information

    Preferred Names
    dihydrofolate reductase
    NP_034179.1

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_010049.3NP_034179.1  dihydrofolate reductase

      See identical proteins and their annotated locations for NP_034179.1

      Status: VALIDATED

      Source sequence(s)
      AI854085, AK031241, BU058996, CAAA01146114, CB247191
      Consensus CDS
      CCDS26680.1
      UniProtKB/Swiss-Prot
      P00375, P70693, Q61485, Q61487, Q61579
      UniProtKB/TrEMBL
      Q544T5
      Related
      ENSMUSP00000022218.5, ENSMUST00000022218.6
      Conserved Domains (1) summary
      cd00209
      Location:5184
      DHFR; Dihydrofolate reductase (DHFR). Reduces 7,8-dihydrofolate to 5,6,7,8-tetrahydrofolate with NADPH as a cofactor. This is an essential step in the biosynthesis of deoxythymidine phosphate since 5,6,7,8-tetrahydrofolate is required to regenerate 5, ...

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000079.7 Reference GRCm39 C57BL/6J

      Range
      92491291..92525561
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
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