slmb supernumerary limbs [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: slmbprovided by FlyBase
Official Full Name: supernumerary limbsprovided by FlyBase
Primary source: FLYBASE:FBgn0283468
Locus tag: Dmel_CG3412
See related: AllianceGenome:FB:FBgn0283468
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: BcDNA:GM02031; beta-TrCP; betaTrCP; CG3412; crd; Dmel\CG3412; FBXW1; ica; l(3)00295; MENE (3R)-B; MENE(3R)-B; shv; slimb; Slimb; SLIMB; Slimb/beta-TrCP; Slimb/beta-TRCP; Slimb/Beta-TRCP1; Slimb/betaTrCP; Slmb; SLMB; wel
Summary: Enables beta-catenin destruction complex binding activity; phosphoprotein binding activity; and ubiquitin-like ligase-substrate adaptor activity. Involved in several processes, including negative regulation of signal transduction; oogenesis; and regulation of organelle organization. Part of SCF ubiquitin ligase complex. Is expressed in several structures, including antennal anlage; anterior endoderm anlage; follicle cell; ganglia; and organism subdivision. Used to study Menkes disease. Orthologous to several human genes including BTRC (beta-transducin repeat containing E3 ubiquitin protein ligase). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 93B13-93C1; 3-70 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (21119252..21127485, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (16944974..16953207, complement)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

SCF-Slimb is critical for Glycogen synthase kinase-3beta-mediated suppression of TAF15-induced neurotoxicity in Drosophila.
Title: SCF-Slimb is critical for Glycogen synthase kinase-3β-mediated suppression of TAF15-induced neurotoxicity in Drosophila.
Wnt regulation: exploring Axin-Disheveled interactions and defining mechanisms by which the SCF E3 ubiquitin ligase is recruited to the destruction complex.
Title: Wnt regulation: exploring Axin-Disheveled interactions and defining mechanisms by which the SCF E3 ubiquitin ligase is recruited to the destruction complex.
The E3 ubiquitin ligase Slimb/beta-TrCP is required for normal copper homeostasis in Drosophila.
Title: The E3 ubiquitin ligase Slimb/β-TrCP is required for normal copper homeostasis in Drosophila.
Here, the authors show that the Casein Kinase 1 (CKI) family is required for Expanded phosphorylation. CKI expression promotes Expanded phosphorylation and interaction with Slimb/beta-TrCP.
Title: Casein kinase 1 family proteins promote Slimb-dependent Expanded degradation.
Our data revealed a new link to understand the underlying mechanism for Nerfin-1 turnover in post-translational level, and provided useful insights in animal development and disease treatment by manipulating the activity of Slimb and Nerfin-1.
Title: The SCF ubiquitin ligase Slimb controls Nerfin-1 turnover in Drosophila.
Interaction with Slimb leads to the ubiquitination and degradation of dSmurf, resulting in negative regulation of dSmurf-mediated Yki phosphorylation and activity in the Hippo pathway. Thus our study revealed a new regulatory mechanism of the Hippo pathway which may provide implications for developing tumor treatment.
Title: The Drosophila F-box protein Slimb controls dSmurf protein turnover to regulate the Hippo pathway.
When Asl reduction is attenuated by Asl overexpression, plk4 mutations, Plk4 RNAi, or Slimb overexpression, Asl levels are higher in spermatozoa, resulting in embryos with reduced viability.
Title: Asterless Reduction during Spermiogenesis Is Regulated by Plk4 and Is Essential for Zygote Development in Drosophila.
Slimb targets Bax and Debcl to the proteasome.
Title: The drosophila Bcl-2 family protein Debcl is targeted to the proteasome by the β-TrCP homologue slimb.
Slmb has a role in control of oocyte and epithelial polarity by antagonizing the aPKC/Par-6 complex
Title: Slmb antagonises the aPKC/Par-6 complex to control oocyte and epithelial polarity.
Slmb has a role in restricting the activity of aPKC to polarize epithelial cells
Title: The F-box protein Slmb restricts the activity of aPKC to polarize epithelial cells.

Submit: New GeneRIF Correction See all GeneRIFs (20)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables beta-catenin destruction complex binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphoprotein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein dimerization activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin-like ligase-substrate adaptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin-like ligase-substrate adaptor activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin-like ligase-substrate adaptor activity	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process	ISM Inferred from Sequence Model more info
involved_in chorion-containing eggshell formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in circadian rhythm	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in circadian rhythm	IPI Inferred from Physical Interaction more info	PubMed
involved_in dorsal appendage formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of epithelial cell polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in follicle cell of egg chamber development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in follicle cell of egg chamber stalk formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germarium-derived egg chamber formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germarium-derived female germ-line cyst encapsulation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germarium-derived female germ-line cyst formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in locomotor rhythm	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of antimicrobial peptide production	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of canonical Wnt signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cell population proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of glial cell proliferation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of hippo signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of hippo signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of insulin receptor signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of nurse cell apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of peptidoglycan recognition protein signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction	IPI Inferred from Physical Interaction more info	PubMed
involved_in negative regulation of smoothened signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of smoothened signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of smoothened signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oocyte axis specification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oocyte localization involved in germarium-derived egg chamber formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of Toll signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of border follicle cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of neuron remodeling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein catabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of synaptonemal complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein K11-linked ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein polyubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein polyubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein polyubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteolysis	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteolysis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of centriole replication	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of chromosome condensation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of establishment of cell polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mitotic nuclear division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of proteasomal protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of SCF ubiquitin ligase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of SCF ubiquitin ligase complex	IDA Inferred from Direct Assay more info	PubMed
part_of SCF ubiquitin ligase complex	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: supernumerary limbs

Names: CG3412-PA; CG3412-PB; CG3412-PC; SCF-betaTrCP complex; SLIMB; centrosome replication defective; icare; pingiel; shiva; slmb-PA; slmb-PB; slmb-PC; wellman

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

21119252..21127485 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_079706.3 → NP_524430.1 supernumerary limbs, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_524430.1

Status: REVIEWED

UniProtKB/TrEMBL

O44382, Q9VDE3

Related

FBpp0083434

Conserved Domains (5) summary

COG2319
Location:192 → 495

WD40; WD40 repeat [General function prediction only]

cd00200
Location:210 → 488

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

pfam12125
Location:47 → 85

Beta-TrCP_D; D domain of beta-TrCP

pfam12937
Location:89 → 136

F-box-like; F-box-like

sd00039
Location:213 → 248

7WD40; WD40 repeat [structural motif]
NM_001275873.1 → NP_001262802.1 supernumerary limbs, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001262802.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KHK1, O44382

Conserved Domains (5) summary

COG2319
Location:192 → 495

WD40; WD40 repeat [General function prediction only]

cd00200
Location:210 → 488

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

pfam12125
Location:47 → 85

Beta-TrCP_D; D domain of beta-TrCP

pfam12937
Location:89 → 136

F-box-like; F-box-like

sd00039
Location:213 → 248

7WD40; WD40 repeat [structural motif]
NM_001275872.1 → NP_001262801.1 supernumerary limbs, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001262801.1

Status: REVIEWED

UniProtKB/TrEMBL

O44382, Q9VDE3

Conserved Domains (5) summary

COG2319
Location:192 → 495

WD40; WD40 repeat [General function prediction only]

cd00200
Location:210 → 488

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

pfam12125
Location:47 → 85

Beta-TrCP_D; D domain of beta-TrCP

pfam12937
Location:89 → 136

F-box-like; F-box-like

sd00039
Location:213 → 248

7WD40; WD40 repeat [structural motif]