Duox Dual oxidase [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Duoxprovided by FlyBase
Official Full Name: Dual oxidaseprovided by FlyBase
Primary source: FLYBASE:FBgn0283531
Locus tag: Dmel_CG3131
See related: AllianceGenome:FB:FBgn0283531
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG3131; Cy; dduox; dDuox; Dmel\CG3131; duox; duOx; DuoX; DUOX; l(2)23Bb
Summary: Enables NAD(P)H oxidase H2O2-forming activity; peroxidase activity; and superoxide-generating NADPH oxidase activity. Involved in several processes, including adult chitin-based cuticle development; adult chitin-containing cuticle pigmentation; and imaginal disc-derived wing morphogenesis. Located in endomembrane system. Is expressed in adult head and embryonic/larval fat body. Used to study primary bacterial infectious disease. Human ortholog(s) of this gene implicated in congenital hypothyroidism and thyroid dyshormonogenesis 6. Orthologous to several human genes including DUOX2 (dual oxidase 2). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 23B2-23B3; 2-7 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (2815970..2830248, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (2815970..2830248, complement)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Duox and Jak/Stat signalling influence disease tolerance in Drosophila during Pseudomonas entomophila infection.
Title: Duox and Jak/Stat signalling influence disease tolerance in Drosophila during Pseudomonas entomophila infection.
Duox-generated reactive oxygen species activate ATR/Chk1 to induce G2 arrest in Drosophila tracheoblasts.
Title: Duox-generated reactive oxygen species activate ATR/Chk1 to induce G2 arrest in Drosophila tracheoblasts.
our results propose that the uracil/Duox pathway promotes bacteria expulsion from the gut through the HOCl-sensitive receptor, TRPA1(A)10b, thereby minimizing the chances that bacteria adapt to survive host defense systems.
Title: TrpA1 Regulates Defecation of Food-Borne Pathogens under the Control of the Duox Pathway.
wound-induced calcium flash activates DUOX
Title: Calcium flashes orchestrate the wound inflammatory response through DUOX activation and hydrogen peroxide release.
One gene required to activate epidermal wound reporters encodes Dual oxidase, an enzyme that produces hydrogen peroxide.
Title: Duox, Flotillin-2, and Src42A are required to activate or delimit the spread of the transcriptional response to epidermal wounds in Drosophila.
dDuox plays important roles in the stabilization of the cuticle structure of the wings.
Title: Essential role of Duox in stabilization of Drosophila wing.
the Galphaq-PLCbeta-Ca(2+)-DUOX-ROS signaling pathway acts as a bona fide first line of defense that enables gut epithelia to dynamically control yeast during the Drosophila life cycle
Title: Regulation of DUOX by the Galphaq-phospholipase Cbeta-Ca2+ pathway in Drosophila gut immunity.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables NAD(P)H oxidase H2O2-forming activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables heme binding	IEA Inferred from Electronic Annotation more info
enables peroxidase activity	IDA Inferred from Direct Assay more info	PubMed
enables peroxidase activity	IEA Inferred from Electronic Annotation more info
enables superoxide-generating NAD(P)H oxidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables superoxide-generating NADPH oxidase activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in adult chitin-based cuticle development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in adult chitin-containing cuticle pigmentation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cuticle development	ISS Inferred from Sequence or Structural Similarity more info
involved_in cytokine-mediated signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in defense response	IBA Inferred from Biological aspect of Ancestor more info
involved_in defense response to bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in imaginal disc-derived wing morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mucosal immune response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in reactive oxygen species metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to cAMP	ISS Inferred from Sequence or Structural Similarity more info
involved_in response to oxidative stress	IEA Inferred from Electronic Annotation more info
involved_in response to wounding	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in superoxide anion generation	IBA Inferred from Biological aspect of Ancestor more info
involved_in wing disc development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of NADPH oxidase complex	IBA Inferred from Biological aspect of Ancestor more info
located_in endomembrane system	HDA more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: dual oxidase

Names: CG3131-PB; CG3131-PC; Duox-PB; Duox-PC; NADPH dual oxidase; curly

NP_001259968.1

EC 1.-.-.-

EC 1.11.1.-

EC 1.6.3.1

NP_608715.2

EC 1.-.-.-

EC 1.11.1.-

EC 1.6.3.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

2815970..2830248 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001273039.1 → NP_001259968.1 dual oxidase, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001259968.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q6AWK1, Q9VQH2

UniProtKB/TrEMBL

D0Z752, M9PCF1

Conserved Domains (6) summary

cd06186
Location:1265 → 1536

NOX_Duox_like_FAD_NADP; NADPH oxidase (NOX) catalyzes the generation of reactive oxygen species (ROS) such as superoxide and hydrogen peroxide. ROS were originally identified as bactericidal agents in phagocytes, but are now also implicated in cell signaling and metabolism. NOX ...

cd09820
Location:72 → 625

dual_peroxidase_like; Dual oxidase and related animal heme peroxidases

cd00051
Location:860 → 921

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam01794
Location:1084 → 1217

Ferric_reduct; Ferric reductase like transmembrane component

pfam03098
Location:70 → 580

An_peroxidase; Animal haem peroxidase

pfam13499
Location:899 → 965

EF-hand_7; EF-hand domain pair
NM_134871.3 → NP_608715.2 dual oxidase, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_608715.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q6AWK1, Q9VQH2

UniProtKB/TrEMBL

D0Z752, M9PCF1

Conserved Domains (6) summary

cd06186
Location:1265 → 1536

NOX_Duox_like_FAD_NADP; NADPH oxidase (NOX) catalyzes the generation of reactive oxygen species (ROS) such as superoxide and hydrogen peroxide. ROS were originally identified as bactericidal agents in phagocytes, but are now also implicated in cell signaling and metabolism. NOX ...

cd09820
Location:72 → 625

dual_peroxidase_like; Dual oxidase and related animal heme peroxidases

cd00051
Location:860 → 921

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam01794
Location:1084 → 1217

Ferric_reduct; Ferric reductase like transmembrane component

pfam03098
Location:70 → 580

An_peroxidase; Animal haem peroxidase

pfam13499
Location:899 → 965

EF-hand_7; EF-hand domain pair