Mi-2 Mi-2 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Mi-2provided by FlyBase
Official Full Name: Mi-2provided by FlyBase
Primary source: FLYBASE:FBgn0262519
Locus tag: Dmel_CG8103
See related: AllianceGenome:FB:FBgn0262519
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: 0006/06; 0854/01; CG8103; Chd4; Dm-Mi-2; Dmel\CG8103; dMi; dMi-2; dMI-2; dMi2; hip76; l(3)01058; l(3)76BDe; l(3)A154.3M3; l(3)j3D4; l(3)L1243; l(3)S000606; l(3)S085401; M-i2; Mi 2; MI-2; Mi2; MI2; pha; Pha
Summary: Enables ATP hydrolysis activity; chromatin binding activity; and nucleosome array spacer activity. Involved in several processes, including chromatin remodeling; chromosome organization; and negative regulation of transcription by RNA polymerase II. Located in euchromatin; nucleus; and polytene chromosome. Is expressed in several structures, including adult brain cell body rind; adult head; germline cell; gut section; and synaptic neuropil domain. Human ortholog(s) of this gene implicated in Sifrim-Hitz-Weiss syndrome; colorectal cancer (multiple); lung cancer (multiple); and lymphoma. Orthologous to several human genes including CHD3 (chromodomain helicase DNA binding protein 3). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 76D3-76D4; 3-46 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3L	NT_037436.4 (19882051..19910215, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3L	NT_037436.3 (19875151..19903315, complement)

Chromosome 3L - NT_037436.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The Mi-2 nucleosome remodeler and the Rpd3 histone deacetylase are involved in piRNA-guided heterochromatin formation.
Title: The Mi-2 nucleosome remodeler and the Rpd3 histone deacetylase are involved in piRNA-guided heterochromatin formation.
Mi-2/NuRD complex protects stem cell progeny from mitogenic Notch signaling.
Title: Mi-2/NuRD complex protects stem cell progeny from mitogenic Notch signaling.
Results of genetic interaction analyses revealed that Mi-2 and Caf1/p55, components of the Nucleosome Remodeling and Deacetylase (NuRD) complex, genetically antagonize the role of DREF in germline stem cell maintenance. Taken together, these data suggest that DREF contributes to intrinsic components of the germline stem cell regulatory network that maintains competence to self-renew.
Title: DREF Genetically Counteracts Mi-2 and Caf1 to Regulate Adult Stem Cell Maintenance.
Genetic experiments in Drosophila demonstrate that expression of cancer-derived dMi-2 mutants misregulates differentiation of epithelial wing structures and produces phenotypes that correlate with their nucleosome remodelling properties.
Title: Tumour-associated missense mutations in the dMi-2 ATPase alters nucleosome remodelling properties in a mutation-specific manner.
EcR and dMi-2 form a complex that is devoid of Ultraspiracle. Unexpectedly, EcR contacts the dMi-2 ATPase domain and increases the efficiency of dMi-2-mediated nucleosome remodelling
Title: EcR recruits dMi-2 and increases efficiency of dMi-2-mediated remodelling to constrain transcription of hormone-regulated genes.
dMi-2 is an important regulator of both chromosome condensation and cohesin binding in interphase cells.
Title: The Drosophila MI-2 chromatin-remodeling factor regulates higher-order chromatin structure and cohesin dynamics in vivo.
The results suggest that dMi-2 distribution over active heat shock genes are determined by transcriptional activity.
Title: Recruitment of the ATP-dependent chromatin remodeler dMi-2 to the transcribed region of active heat shock genes.
Results suggest that Drosophila Mi-2/NuRD complex functions in neuronal differentiation through the repression of proneural gene expression by chromatin remodeling and histone deacetylation.
Title: Mi-2 chromatin remodeling factor functions in sensory organ development through proneural gene repression in Drosophila.
chromodomains are DNA binding modules important for ATP-dependent nucleosome mobilization
Title: The dMi-2 chromodomains are DNA binding modules important for ATP-dependent nucleosome mobilization.
results reveal a potential mechanism for regulation of the dMi-2 enzyme and point toward CK2 phosphorylation as a common feature of CHD family ATPases
Title: dMi-2 chromatin binding and remodeling activities are regulated by dCK2 phosphorylation.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Dm0951 (e-PCR)
- UniSTS:66795

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATP hydrolysis activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP-dependent chromatin remodeler activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATP-dependent chromatin remodeler activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP-dependent chromatin remodeler activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables chromatin binding	IBA Inferred from Biological aspect of Ancestor more info
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables histone binding	IBA Inferred from Biological aspect of Ancestor more info
enables nucleosome array spacer activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables zinc ion binding	ISM Inferred from Sequence Model more info	PubMed

Process	Evidence Code	Pubs
involved_in chromatin remodeling	IBA Inferred from Biological aspect of Ancestor more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in chromosome condensation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromosome organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic sister chromatid cohesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA-templated transcription	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in nucleosome organization	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transposable element silencing by piRNA-mediated heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of NuRD complex	ISS Inferred from Sequence or Structural Similarity more info	PubMed
part_of NuRD complex	TAS Traceable Author Statement more info	PubMed
located_in chromatin	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	HDA more info	PubMed
located_in euchromatin	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in polytene chromosome	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Mi-2

Names: CG8103-PA; CG8103-PB; CG8103-PC; CG8103-PD; Mi-2 ortholog; Mi-2-PA; Mi-2-PB; Mi-2-PC; Mi-2-PD; PHD-containing ATPase; dMi-2; hb-interacting protein 76; lethal (3) L1243

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_037436.4 Reference assembly

Range

19882051..19910215 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_140897.4 → NP_649154.2 Mi-2, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_649154.2

Status: REVIEWED

UniProtKB/Swiss-Prot

O97159, Q8MZ43, Q9VW50

UniProtKB/TrEMBL

E1JI46

Related

FBpp0074688

Conserved Domains (11) summary

smart00298
Location:614 → 666

CHROMO; Chromatin organization modifier domain

cd00024
Location:489 → 557

CHROMO; Chromatin organization modifier (chromo) domain is a conserved region of around 50 amino acids found in a variety of chromosomal proteins, which appear to play a role in the functional organization of the eukaryotic nucleus. Experimental evidence ...

cd00046
Location:750 → 906

DEXDc; DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.

cd15531
Location:379 → 421

PHD1_CHD_II; PHD finger 1 found in class II Chromodomain-Helicase-DNA binding (CHD) proteins

cd15532
Location:439 → 481

PHD2_CHD_II; PHD finger 2 found in class II Chromodomain-Helicase-DNA binding (CHD) proteins

pfam00176
Location:733 → 1027

SNF2_N; SNF2 family N-terminal domain

pfam00271
Location:1052 → 1166

Helicase_C; Helicase conserved C-terminal domain

pfam06461
Location:1379 → 1516

DUF1086; Domain of Unknown Function (DUF1086)

pfam06465
Location:1291 → 1336

DUF1087; Domain of Unknown Function (DUF1087)

pfam08073
Location:137 → 189

CHDNT; CHDNT (NUC034) domain

pfam08074
Location:1755 → 1926

CHDCT2; CHDCT2 (NUC038) domain
NM_001170005.2 → NP_001163476.1 Mi-2, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001163476.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O97159, Q8MZ43, Q9VW50

UniProtKB/TrEMBL

E1JI46

Conserved Domains (11) summary

smart00298
Location:614 → 666

CHROMO; Chromatin organization modifier domain

cd00024
Location:489 → 557

CHROMO; Chromatin organization modifier (chromo) domain is a conserved region of around 50 amino acids found in a variety of chromosomal proteins, which appear to play a role in the functional organization of the eukaryotic nucleus. Experimental evidence ...

cd00046
Location:750 → 906

DEXDc; DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.

cd15531
Location:379 → 421

PHD1_CHD_II; PHD finger 1 found in class II Chromodomain-Helicase-DNA binding (CHD) proteins

cd15532
Location:439 → 481

PHD2_CHD_II; PHD finger 2 found in class II Chromodomain-Helicase-DNA binding (CHD) proteins

pfam00176
Location:733 → 1027

SNF2_N; SNF2 family N-terminal domain

pfam00271
Location:1052 → 1166

Helicase_C; Helicase conserved C-terminal domain

pfam06461
Location:1379 → 1516

DUF1086; Domain of Unknown Function (DUF1086)

pfam06465
Location:1291 → 1336

DUF1087; Domain of Unknown Function (DUF1087)

pfam08073
Location:137 → 189

CHDNT; CHDNT (NUC034) domain

pfam08074
Location:1755 → 1926

CHDCT2; CHDCT2 (NUC038) domain
NM_001014591.3 → NP_001014591.1 Mi-2, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001014591.1

Status: REVIEWED

UniProtKB/TrEMBL

Q59E34

Conserved Domains (11) summary

smart00298
Location:615 → 667

CHROMO; Chromatin organization modifier domain

cd00024
Location:490 → 558

CHROMO; Chromatin organization modifier (chromo) domain is a conserved region of around 50 amino acids found in a variety of chromosomal proteins, which appear to play a role in the functional organization of the eukaryotic nucleus. Experimental evidence ...

cd00046
Location:751 → 907

DEXDc; DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.

cd15531
Location:380 → 422

PHD1_CHD_II; PHD finger 1 found in class II Chromodomain-Helicase-DNA binding (CHD) proteins

cd15532
Location:440 → 482

PHD2_CHD_II; PHD finger 2 found in class II Chromodomain-Helicase-DNA binding (CHD) proteins

pfam00176
Location:734 → 1028

SNF2_N; SNF2 family N-terminal domain

pfam00271
Location:1053 → 1167

Helicase_C; Helicase conserved C-terminal domain

pfam06461
Location:1380 → 1517

DUF1086; Domain of Unknown Function (DUF1086)

pfam06465
Location:1292 → 1337

DUF1087; Domain of Unknown Function (DUF1087)

pfam08073
Location:138 → 190

CHDNT; CHDNT (NUC034) domain

pfam08074
Location:1756 → 1927

CHDCT2; CHDCT2 (NUC038) domain
NM_001275149.1 → NP_001262078.1 Mi-2, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001262078.1

Status: REVIEWED

UniProtKB/TrEMBL

M9PIA6

Conserved Domains (11) summary

smart00298
Location:605 → 657

CHROMO; Chromatin organization modifier domain

cd00024
Location:480 → 548

CHROMO; Chromatin organization modifier (chromo) domain is a conserved region of around 50 amino acids found in a variety of chromosomal proteins, which appear to play a role in the functional organization of the eukaryotic nucleus. Experimental evidence ...

cd00046
Location:741 → 897

DEXDc; DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.

cd15531
Location:370 → 412

PHD1_CHD_II; PHD finger 1 found in class II Chromodomain-Helicase-DNA binding (CHD) proteins

cd15532
Location:430 → 472

PHD2_CHD_II; PHD finger 2 found in class II Chromodomain-Helicase-DNA binding (CHD) proteins

pfam00176
Location:724 → 1018

SNF2_N; SNF2 family N-terminal domain

pfam00271
Location:1043 → 1157

Helicase_C; Helicase conserved C-terminal domain

pfam06461
Location:1370 → 1507

DUF1086; Domain of Unknown Function (DUF1086)

pfam06465
Location:1282 → 1327

DUF1087; Domain of Unknown Function (DUF1087)

pfam08073
Location:128 → 180

CHDNT; CHDNT (NUC034) domain

pfam08074
Location:1746 → 1917

CHDCT2; CHDCT2 (NUC038) domain