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    Nos3 nitric oxide synthase 3 [ Rattus norvegicus (Norway rat) ]

    Gene ID: 24600, updated on 9-Dec-2024

    Summary

    Official Symbol
    Nos3provided by RGD
    Official Full Name
    nitric oxide synthase 3provided by RGD
    Primary source
    RGD:3186
    See related
    EnsemblRapid:ENSRNOG00000009348 AllianceGenome:RGD:3186
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Rattus norvegicus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
    Also known as
    eNos
    Summary
    Enables several functions, including Hsp90 protein binding activity; beta-catenin binding activity; and calmodulin binding activity. Involved in several processes, including cellular response to cAMP; cellular response to magnesium ion; and cellular response to mechanical stimulus. Located in several cellular components, including apical part of cell; caveola; and sarcolemma. Used to study artery disease (multiple); irritable bowel syndrome; ischemia (multiple); liver disease (multiple); and retinal disease (multiple). Biomarker of several diseases, including artery disease (multiple); kidney failure (multiple); liver disease (multiple); lung disease (multiple); and overnutrition (multiple). Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); eye disease (multiple); leukemia (multiple); reproductive organ cancer (multiple); and vasculitis (multiple). Orthologous to human NOS3 (nitric oxide synthase 3). [provided by Alliance of Genome Resources, Dec 2024]
    Expression
    Biased expression in Heart (RPKM 114.2), Spleen (RPKM 51.1) and 8 other tissues See more
    Orthologs
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    See Nos3 in Genome Data Viewer
    Location:
    4q11
    Exon count:
    26
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCr8 (GCF_036323735.1) 4 NC_086022.1 (11686088..11706604, complement)
    RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 4 NC_051339.1 (10793834..10814170, complement)
    106 previous assembly Rnor_6.0 (GCF_000001895.5) 4 NC_005103.4 (7321908..7342404, complement)

    Chromosome 4 - NC_086022.1Genomic Context describing neighboring genes Neighboring gene ATP binding cassette subfamily B member 8 Neighboring gene uncharacterized LOC120102208 Neighboring gene autophagy related 9B Neighboring gene potassium voltage-gated channel subfamily H member 2 Neighboring gene uncharacterized LOC108350647

    Genomic regions, transcripts, and products

    Expression

    • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
    • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
    • BioProject: PRJNA238328
    • Publication: PMID 24510058
    • Analysis date: Mon Jun 6 17:44:12 2016

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    Pathways from PubChem

    General gene information

    Markers

    Gene Ontology Provided by RGD

    Function Evidence Code Pubs
    enables FMN binding IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables FMN binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables Hsp90 protein binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables NADP binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables actin binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables actin monomer binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables actin monomer binding ISO
    Inferred from Sequence Orthology
    more info
     
    enables arginine binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables arginine binding ISO
    Inferred from Sequence Orthology
    more info
     
    enables beta-catenin binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables cadherin binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables calmodulin binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables calmodulin binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables flavin adenine dinucleotide binding IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables flavin adenine dinucleotide binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables heme binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables heme binding ISO
    Inferred from Sequence Orthology
    more info
     
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables nitric-oxide synthase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables nitric-oxide synthase activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables nitric-oxide synthase activity IEA
    Inferred from Electronic Annotation
    more info
     
    enables nitric-oxide synthase activity IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    enables nitric-oxide synthase activity ISO
    Inferred from Sequence Orthology
    more info
     
    enables nitric-oxide synthase binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables scaffold protein binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables scaffold protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables scaffold protein binding ISO
    Inferred from Sequence Orthology
    more info
     
    enables tetrahydrobiopterin binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables tetrahydrobiopterin binding ISO
    Inferred from Sequence Orthology
    more info
     
    Process Evidence Code Pubs
    involved_in angiogenesis IEA
    Inferred from Electronic Annotation
    more info
     
    acts_upstream_of_or_within angiogenesis ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in aortic valve morphogenesis IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in aortic valve morphogenesis ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in arginine catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in arginine catabolic process IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in arginine catabolic process ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in blood vessel diameter maintenance IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in blood vessel diameter maintenance ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in blood vessel remodeling IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in blood vessel remodeling ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in bone development IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in calcium ion transport IEA
    Inferred from Electronic Annotation
    more info
     
    acts_upstream_of calcium ion transport ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in cellular response to cAMP IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in cellular response to growth factor stimulus IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in cellular response to heat IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in cellular response to high density lipoprotein particle stimulus IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in cellular response to insulin stimulus IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in cellular response to low-density lipoprotein particle stimulus IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in cellular response to magnesium ion IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in cellular response to mechanical stimulus IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in cellular response to organic cyclic compound IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in cellular response to rotenone IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in cellular response to transforming growth factor beta stimulus IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in cellular response to tumor necrosis factor IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in endocardial cushion morphogenesis IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in endocardial cushion morphogenesis ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in endothelial cell migration IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in endothelial cell migration ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in establishment of localization in cell IEA
    Inferred from Electronic Annotation
    more info
     
    acts_upstream_of establishment of localization in cell ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in female pregnancy IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in homeostasis of number of cells within a tissue IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in homeostasis of number of cells within a tissue ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in in utero embryonic development IEA
    Inferred from Electronic Annotation
    more info
     
    acts_upstream_of_or_within in utero embryonic development ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in lipopolysaccharide-mediated signaling pathway IEA
    Inferred from Electronic Annotation
    more info
     
    acts_upstream_of_or_within lipopolysaccharide-mediated signaling pathway ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in lung development IEA
    Inferred from Electronic Annotation
    more info
     
    acts_upstream_of_or_within lung development ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in negative regulation of biomineral tissue development IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in negative regulation of biomineral tissue development ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in negative regulation of blood pressure IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in negative regulation of calcium ion transport IEA
    Inferred from Electronic Annotation
    more info
     
    acts_upstream_of_or_within negative regulation of calcium ion transport ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in negative regulation of cell population proliferation ISO
    Inferred from Sequence Orthology
    more info
     
    acts_upstream_of_or_within negative regulation of hydrolase activity ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in negative regulation of muscle hyperplasia IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in negative regulation of muscle hyperplasia ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in negative regulation of potassium ion transport IEA
    Inferred from Electronic Annotation
    more info
     
    acts_upstream_of_or_within negative regulation of potassium ion transport ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in negative regulation of smooth muscle cell proliferation IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in negative regulation of smooth muscle cell proliferation IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    acts_upstream_of_or_within negative regulation of smooth muscle cell proliferation ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in nitric oxide biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in nitric oxide biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in nitric oxide biosynthetic process IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in nitric oxide biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    acts_upstream_of_or_within nitric oxide biosynthetic process ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in nitric oxide biosynthetic process ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in nitric oxide mediated signal transduction IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in nitric oxide metabolic process IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in ovulation from ovarian follicle IEA
    Inferred from Electronic Annotation
    more info
     
    acts_upstream_of_or_within ovulation from ovarian follicle ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in positive regulation of angiogenesis IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in positive regulation of angiogenesis ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in positive regulation of apoptotic process IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in positive regulation of blood vessel endothelial cell migration IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in positive regulation of blood vessel endothelial cell migration ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in positive regulation of gene expression IEA
    Inferred from Electronic Annotation
    more info
     
    acts_upstream_of_positive_effect positive regulation of gene expression ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in positive regulation of guanylate cyclase activity ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in potassium ion transport IEA
    Inferred from Electronic Annotation
    more info
     
    acts_upstream_of potassium ion transport ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in pulmonary valve morphogenesis IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in pulmonary valve morphogenesis ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in regulation of nervous system process IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in regulation of nervous system process ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in regulation of sodium ion transport IEA
    Inferred from Electronic Annotation
    more info
     
    acts_upstream_of_or_within regulation of sodium ion transport ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in regulation of systemic arterial blood pressure by endothelin IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in regulation of systemic arterial blood pressure by endothelin ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in regulation of the force of heart contraction by chemical signal IEA
    Inferred from Electronic Annotation
    more info
     
    acts_upstream_of_or_within regulation of the force of heart contraction by chemical signal ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in removal of superoxide radicals IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in removal of superoxide radicals ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in response to L-arginine IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to activity IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to amino acid IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to axon injury IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to carbohydrate IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to corticosterone IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to cytokine IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to erythropoietin IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to estradiol IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in response to estradiol IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to ethanol IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to fluid shear stress IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in response to fluid shear stress ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in response to fructose IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to hormone IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in response to hyperoxia IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to hypoxia IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to ketone IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to lead ion IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to lipopolysaccharide IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in response to lipopolysaccharide IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to magnetism IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to mechanical stimulus IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to mercury ion IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to metal ion IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to nicotine IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to nutrient levels IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to organic cyclic compound IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to peptide IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to peptide hormone IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to salt IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to ultrasound IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in response to xenobiotic stimulus IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in retina development in camera-type eye IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in smooth muscle hyperplasia IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in smooth muscle hyperplasia ISO
    Inferred from Sequence Orthology
    more info
     
    acts_upstream_of_or_within vasodilation ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in ventricular septum morphogenesis IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in ventricular septum morphogenesis ISO
    Inferred from Sequence Orthology
    more info
     
    Component Evidence Code Pubs
    located_in Golgi apparatus IEA
    Inferred from Electronic Annotation
    more info
     
    located_in Golgi apparatus ISO
    Inferred from Sequence Orthology
    more info
     
    located_in apical part of cell IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in caveola IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in caveola IEA
    Inferred from Electronic Annotation
    more info
     
    located_in caveola ISO
    Inferred from Sequence Orthology
    more info
     
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in cytoplasm ISO
    Inferred from Sequence Orthology
    more info
     
    located_in cytoskeleton IEA
    Inferred from Electronic Annotation
    more info
     
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
     
    located_in nucleus ISO
    Inferred from Sequence Orthology
    more info
     
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in plasma membrane ISO
    Inferred from Sequence Orthology
    more info
     
    located_in sarcolemma IDA
    Inferred from Direct Assay
    more info
    PubMed 

    General protein information

    Preferred Names
    nitric oxide synthase 3
    Names
    EC-NOS
    NOS type III
    NOSIII
    cNOS
    constitutive NOS
    endothelial NOS
    endothelial nitric oxide synthase 3
    nitric oxide synthase 3, endothelial cell
    nitric oxide synthase, endothelial
    NP_068610.1
    XP_006235934.1

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_021838.2NP_068610.1  nitric oxide synthase 3

      See identical proteins and their annotated locations for NP_068610.1

      Status: PROVISIONAL

      Source sequence(s)
      AB176831
      UniProtKB/TrEMBL
      A0A8I6A2Y1
      Related
      ENSRNOP00000013058.4, ENSRNOT00000013058.6
      Conserved Domains (5) summary
      COG0369
      Location:5111158
      CysJ; Sulfite reductase, alpha subunit (flavoprotein) [Inorganic ion transport and metabolism]
      cd00795
      Location:67479
      NOS_oxygenase_euk; Nitric oxide synthase (NOS) eukaryotic oxygenase domain. NOS produces nitric oxide (NO) by catalyzing a five-electron heme-based oxidation of a guanidine nitrogen of L-arginine to L-citrulline via two successive monooxygenation reactions producing N ...
      pfam00258
      Location:521697
      Flavodoxin_1; Flavodoxin
      pfam01698
      Location:3061
      FLO_LFY; Floricaula / Leafy protein
      cl06868
      Location:7611163
      FNR_like; Ferredoxin reductase (FNR), an FAD and NAD(P) binding protein, was intially identified as a chloroplast reductase activity, catalyzing the electron transfer from reduced iron-sulfur protein ferredoxin to NADP+ as the final step in the electron transport ...

    RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCr8

    Genomic

    1. NC_086022.1 Reference GRCr8

      Range
      11686088..11706604 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006235872.3XP_006235934.1  nitric oxide synthase 3 isoform X1

      UniProtKB/TrEMBL
      A0A8I6A2Y1
      Related
      ENSRNOP00000086104.2, ENSRNOT00000118104.2
      Conserved Domains (4) summary
      COG0369
      Location:4611108
      CysJ; Sulfite reductase, alpha subunit (flavoprotein) [Inorganic ion transport and metabolism]
      cd00795
      Location:17429
      NOS_oxygenase_euk; Nitric oxide synthase (NOS) eukaryotic oxygenase domain. NOS produces nitric oxide (NO) by catalyzing a five-electron heme-based oxidation of a guanidine nitrogen of L-arginine to L-citrulline via two successive monooxygenation reactions producing N ...
      pfam00258
      Location:471647
      Flavodoxin_1; Flavodoxin
      cl06868
      Location:7111113
      FNR_like; Ferredoxin reductase (FNR), an FAD and NAD(P) binding protein, was intially identified as a chloroplast reductase activity, catalyzing the electron transfer from reduced iron-sulfur protein ferredoxin to NADP+ as the final step in the electron transport ...