Axn Axin [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Axnprovided by FlyBase
Official Full Name: Axinprovided by FlyBase
Primary source: FLYBASE:FBgn0026597
Locus tag: Dmel_CG7926
See related: AllianceGenome:FB:FBgn0026597
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: 0442/30; APC; axin; axn; CG7926; d-axin; D-Axin; dAxin; dAXIN; Daxin; DAxin; din; Dmel\CG7926; GS; GSK3beta; l(3)S044230
Summary: Enables several functions, including beta-catenin binding activity; molecular adaptor activity; and protein kinase binding activity. Involved in several processes, including instar larval or pupal development; negative regulation of canonical Wnt signaling pathway; and regionalization. Located in cytoplasm; nucleus; and plasma membrane. Part of Wnt signalosome and beta-catenin destruction complex. Is active in cytosol. Used to study cancer. Human ortholog(s) of this gene implicated in endometrial adenocarcinoma; gastrointestinal system cancer (multiple); lung cancer (multiple); orofacial cleft; and urinary bladder cancer. Orthologous to human AXIN1 (axin 1) and AXIN2 (axin 2). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 99D2-99D3; 3-100 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (30022842..30035311)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (25848564..25861033)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Feedback control of Wnt signaling based on ultrastable histidine cluster co-aggregation between Naked/NKD and Axin.
Title: Feedback control of Wnt signaling based on ultrastable histidine cluster co-aggregation between Naked/NKD and Axin.
Wnt regulation: exploring Axin-Disheveled interactions and defining mechanisms by which the SCF E3 ubiquitin ligase is recruited to the destruction complex.
Title: Wnt regulation: exploring Axin-Disheveled interactions and defining mechanisms by which the SCF E3 ubiquitin ligase is recruited to the destruction complex.
Iduna-mediated regulation of Axin proteolysis is essential for tissue homeostasis in the Drosophila midgut
Title: Axin proteolysis by Iduna is required for the regulation of stem cell proliferation and intestinal homeostasis in Drosophila.
Novel requirement for APC in Axin regulation through phosphorylation both prevents signaling in the Wnt-off state and promotes signaling immediately following Wnt stimulation.
Title: Axin phosphorylation in both Wnt-off and Wnt-on states requires the tumor suppressor APC.
We conclude that even in the unstimulated state, a pool of Axin forms membrane-proximal puncta that are dependent on Apc, and that membrane association regulates both Axin levels and Axin's role in the rapid activation of signaling that follows Wnt exposure.
Title: Dual Roles for Membrane Association of Drosophila Axin in Wnt Signaling.
The pool of ADP-ribosylated Axin, which is degraded under basal conditions, increases immediately following Wnt stimulation in both Drosophila and human cells.
Title: Wnt pathway activation by ADP-ribosylation.
Daxin physically interacts with DTNKS, and its protein levels are elevated in the absence of DTNKS in the eye discs.
Title: The Drosophila tankyrase regulates Wg signaling depending on the concentration of Daxin.
co-expression experiments reveal that APC displaces Dishevelled from Axin assemblies, promoting degradasome over signalosome formation in the absence of Wnts
Title: The Adenomatous polyposis coli tumour suppressor is essential for Axin complex assembly and function and opposes Axin's interaction with Dishevelled.
Data show that Axin and Apc, but not GSK3, modulate the ligand-independent traffic of Notch.
Title: Modulation of the ligand-independent traffic of Notch by Axin and Apc contributes to the activation of Armadillo in Drosophila.
This result implies that Dishevelled has the potential to act as a "natural" dominant-negative, binding to the polymerization interface of Axin's DIX domain to interfere with its self-assembly
Title: Dishevelled interacts with the DIX domain polymerization interface of Axin to interfere with its function in down-regulating β-catenin.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables beta-catenin binding	IBA Inferred from Biological aspect of Ancestor more info
enables beta-catenin binding	IDA Inferred from Direct Assay more info	PubMed
enables beta-catenin binding	IPI Inferred from Physical Interaction more info	PubMed
enables molecular adaptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables molecular adaptor activity	IGI Inferred from Genetic Interaction more info	PubMed
enables protein kinase binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables signaling receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin protein ligase binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in cell development	IBA Inferred from Biological aspect of Ancestor more info
involved_in chitin-based larval cuticle pattern formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dorsal/ventral pattern formation, imaginal disc	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in eye-antennal disc morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in follicle cell of egg chamber development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of glycogen metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heart development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in imaginal disc-derived wing margin morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of canonical Wnt signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of canonical Wnt signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of canonical Wnt signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of canonical Wnt signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of canonical Wnt signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of execution phase of apoptosis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in response to starvation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in segment polarity determination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in somatic stem cell population maintenance	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Wnt signalosome	IDA Inferred from Direct Assay more info	PubMed
part_of beta-catenin destruction complex	IBA Inferred from Biological aspect of Ancestor more info
part_of beta-catenin destruction complex	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: axin

Names: Axn-PA; Axn-PB; Axn-PC; Axn-PD; Axn-PE; Axn-PF; Axn-PG; CG7926-PA; CG7926-PB; CG7926-PC; CG7926-PD; CG7926-PE; CG7926-PF; CG7926-PG; D-axin; Dm axin; axn; dAxin; glycogen synthase kinase 3beta

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

30022842..30035311

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_170457.3 → NP_733336.1 axin, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_733336.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9V407, Q9XYC1

UniProtKB/TrEMBL

A4V3M5, C3KGN7

Related

FBpp0084919

Conserved Domains (3) summary

pfam00778
Location:665 → 739

DIX; DIX domain

pfam08833
Location:494 → 543

Axin_b-cat_bind; Axin beta-catenin binding domain

cl02565
Location:55 → 170

RGS; Regulator of G protein signaling (RGS) domain superfamily
NM_001144654.3 → NP_001138126.1 axin, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001138126.1

Status: REVIEWED

UniProtKB/TrEMBL

B7Z0S0, C3KGN7

Conserved Domains (3) summary

pfam00778
Location:619 → 693

DIX; DIX domain

pfam08833
Location:448 → 497

Axin_b-cat_bind; Axin beta-catenin binding domain

cl02565
Location:55 → 170

RGS; Regulator of G protein signaling (RGS) domain superfamily
NM_001276168.1 → NP_001263097.1 axin, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001263097.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KHW0, C3KGN7

Conserved Domains (3) summary

pfam00778
Location:613 → 687

DIX; DIX domain

pfam08833
Location:448 → 497

Axin_b-cat_bind; Axin beta-catenin binding domain

cl02565
Location:55 → 170

RGS; Regulator of G protein signaling (RGS) domain superfamily
NM_170459.3 → NP_733338.1 axin, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_733338.1

Status: REVIEWED

UniProtKB/TrEMBL

C3KGN7, Q8IMJ7

Related

FBpp0084920

Conserved Domains (3) summary

pfam00778
Location:659 → 733

DIX; DIX domain

pfam08833
Location:494 → 543

Axin_b-cat_bind; Axin beta-catenin binding domain

cl02565
Location:55 → 170

RGS; Regulator of G protein signaling (RGS) domain superfamily
NM_001170302.1 → NP_001163773.1 axin, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001163773.1

Status: REVIEWED

UniProtKB/TrEMBL

E1JJ18

Conserved Domains (2) summary

cl02565
Location:55 → 110

RGS; Regulator of G protein signaling (RGS) domain superfamily

cl16934
Location:19 → 57

Axin_TNKS_binding; Tankyrase binding N-terminal segment of axin
NM_170458.2 → NP_733337.1 axin, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_733337.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9V407, Q9XYC1

UniProtKB/TrEMBL

A4V3M5, C3KGN7

Related

FBpp0084921

Conserved Domains (3) summary

pfam00778
Location:665 → 739

DIX; DIX domain

pfam08833
Location:494 → 543

Axin_b-cat_bind; Axin beta-catenin binding domain

cl02565
Location:55 → 170

RGS; Regulator of G protein signaling (RGS) domain superfamily
NM_001276169.1 → NP_001263098.1 axin, isoform G [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001263098.1

Status: REVIEWED

UniProtKB/TrEMBL

C3KGN7, Q8IMJ7

Conserved Domains (3) summary

pfam00778
Location:659 → 733

DIX; DIX domain

pfam08833
Location:494 → 543

Axin_b-cat_bind; Axin beta-catenin binding domain

cl02565
Location:55 → 170

RGS; Regulator of G protein signaling (RGS) domain superfamily