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    Top1 Topoisomerase 1 [ Drosophila melanogaster (fruit fly) ]

    Gene ID: 32458, updated on 9-Dec-2024

    Summary

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    Official Symbol
    Top1provided by FlyBase
    Official Full Name
    Topoisomerase 1provided by FlyBase
    Primary source
    FLYBASE:FBgn0004924
    Locus tag
    Dmel_CG6146
    See related
    AllianceGenome:FB:FBgn0004924
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Drosophila melanogaster
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
    Also known as
    CG6146; Dmel\CG6146; dTopoI; l(1)G0134; l(1)G0201; l(1)G0229; l(1)G0278; l(1)top1; ND432; top1; TOP1; TopI; topo I; Topo I; Topo1; topoI; TopoI; topoisomerase I
    Summary
    Enables DNA topoisomerase type I (single strand cut, ATP-independent) activity. Involved in several processes, including chromosome organization; instar larval development; and regulation of embryonic development. Located in cytoplasm; euchromatin; and nucleolar chromatin. Is expressed in adult brain cell body rind; organism; and ovary. Orthologous to several human genes including TOP1 (DNA topoisomerase I). [provided by Alliance of Genome Resources, Dec 2024]
    Orthologs
    all
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    Genomic context

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    See Top1 in Genome Data Viewer
    Location:
    13B6-13B6; 1-50 cM
    Exon count:
    10
    Annotation release Status Assembly Chr Location
    Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) X NC_004354.4 (15319479..15328029)
    Release 5.57 previous assembly Release 5 (GCF_000001215.2) X NC_004354.3 (15213512..15222062)

    Chromosome X - NC_004354.4Genomic Context describing neighboring genes Neighboring gene antisense RNA:CR44429 Neighboring gene germ cell-expressed bHLH-PAS Neighboring gene antisense RNA:CR44961 Neighboring gene mir-4957 stem loop Neighboring gene discontinuous actin hexagon Neighboring gene antisense RNA:CR44998 Neighboring gene uncharacterized protein Neighboring gene Histone deacetylase 6

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Dynamic localization of DNA topoisomerase I and its functional relevance during Drosophila development. Huang W, et al. G3 (Bethesda), 2021 Sep 6. PMID 34544118, Free PMC Article
    2. Polyisoprenylated benzophenone, garcinol, a natural histone acetyltransferase inhibitor, represses chromatin transcription and alters global gene expression. Balasubramanyam K, et al. J Biol Chem, 2004 Aug 6. PMID 15155757
    3. Reverse gyrase functions as a DNA renaturase: annealing of complementary single-stranded circles and positive supercoiling of a bubble substrate. Hsieh TS, et al. J Biol Chem, 2006 Mar 3. PMID 16407212
    4. The phosphoCTD-interacting domain of Topoisomerase I. Wu J, et al. Biochem Biophys Res Commun, 2010 Jun 18. PMID 20493173, Free PMC Article
    5. Chromatin structural transitions in Drosophila embryo cell-free extract result in a high conformational flexibility of nucleosomal DNA. Krajewski WA. FEBS Lett, 1999 Jun 11. PMID 10386593

    See all (109) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Dynamic localization of DNA topoisomerase I and its functional relevance during Drosophila development.
      Title: Dynamic localization of DNA topoisomerase I and its functional relevance during Drosophila development.
    2. B52 delivers Topo I to RNA polymerase II-active chromatin loci and DNA topology and mRNA release can be coordinated to control gene expression.
      Title: The SR protein B52/SRp55 is required for DNA topoisomerase I recruitment to chromatin, mRNA release and transcription shutdown.
    3. In a model of human epilepsy, topo I shows a novel function in construction and/or maintenance of circuits required for seizure propagation in vivo.
      Title: Seizure suppression by top1 mutations in Drosophila.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    mobile_element

    • Loc: 15326740-15326927 mobile_element_type = transposon:I{}137

    Gene Ontology Provided by FlyBase

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA topoisomerase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA topoisomerase type I (single strand cut, ATP-independent) activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA topoisomerase type I (single strand cut, ATP-independent) activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA topoisomerase type I (single strand cut, ATP-independent) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA replication IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA topological change IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA topological change IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA topological change IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chromosome condensation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in chromosome segregation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in chromosome segregation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in instar larval development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in oogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of embryonic development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in chromatin IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in chromosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol HDA PubMed 
    located_in euchromatin IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleolar chromatin IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in nucleolus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleolus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    topoisomerase 1
    Names
    CG6146-PA
    CG6146-PB
    CG6146-PC
    CG6146-PD
    CG6146-PE
    Top 1
    Top1-PA
    Top1-PB
    Top1-PC
    Top1-PD
    Top1-PE
    topoisomerase I
    topoisomerase1
    type I topoisomerase
    NP_001014742.1
    NP_001245679.1
    NP_001285267.1
    NP_511161.2
    NP_727841.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_004354.4 Reference assembly

      Range
      15319479..15328029
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_078606.4 → NP_511161.2  topoisomerase 1, isoform A [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_511161.2

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      P30189, Q9VXW6
      UniProtKB/TrEMBL
      Q494M1
      Related
      FBpp0073822
      Conserved Domains (4) summary
      smart00435
      Location:585 → 946
      TOPEUc; DNA Topoisomerase I (eukaryota)
      cd03488
      Location:441 → 655
      Topoisomer_IB_N_htopoI_like; Topoisomer_IB_N_htopoI_like : N-terminal DNA binding fragment found in eukaryotic DNA topoisomerase (topo) IB proteins similar to the monomeric yeast and human topo I. Topo I enzymes are divided into: topo type IA (bacterial) and type IB (eukaryotic). ...
      pfam01028
      Location:657 → 859
      Topoisom_I; Eukaryotic DNA topoisomerase I, catalytic core
      pfam14370
      Location:905 → 972
      Topo_C_assoc; C-terminal topoisomerase domain

    2. NM_001014742.2 → NP_001014742.1  topoisomerase 1, isoform C [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001014742.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      P30189, Q9VXW6
      UniProtKB/TrEMBL
      Q494M1
      Conserved Domains (4) summary
      smart00435
      Location:585 → 946
      TOPEUc; DNA Topoisomerase I (eukaryota)
      cd03488
      Location:441 → 655
      Topoisomer_IB_N_htopoI_like; Topoisomer_IB_N_htopoI_like : N-terminal DNA binding fragment found in eukaryotic DNA topoisomerase (topo) IB proteins similar to the monomeric yeast and human topo I. Topo I enzymes are divided into: topo type IA (bacterial) and type IB (eukaryotic). ...
      pfam01028
      Location:657 → 859
      Topoisom_I; Eukaryotic DNA topoisomerase I, catalytic core
      pfam14370
      Location:905 → 972
      Topo_C_assoc; C-terminal topoisomerase domain

    3. NM_001258750.2 → NP_001245679.1  topoisomerase 1, isoform D [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001245679.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      P30189, Q9VXW6
      UniProtKB/TrEMBL
      M9NDX3
      Conserved Domains (4) summary
      smart00435
      Location:581 → 942
      TOPEUc; DNA Topoisomerase I (eukaryota)
      cd03488
      Location:437 → 651
      Topoisomer_IB_N_htopoI_like; Topoisomer_IB_N_htopoI_like : N-terminal DNA binding fragment found in eukaryotic DNA topoisomerase (topo) IB proteins similar to the monomeric yeast and human topo I. Topo I enzymes are divided into: topo type IA (bacterial) and type IB (eukaryotic). ...
      pfam01028
      Location:653 → 855
      Topoisom_I; Eukaryotic DNA topoisomerase I, catalytic core
      pfam14370
      Location:901 → 968
      Topo_C_assoc; C-terminal topoisomerase domain

    4. NM_167435.3 → NP_727841.1  topoisomerase 1, isoform B [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_727841.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      C6TPA7, Q8IR39
      Related
      FBpp0073823
      Conserved Domains (4) summary
      smart00435
      Location:192 → 553
      TOPEUc; DNA Topoisomerase I (eukaryota)
      cd03488
      Location:48 → 262
      Topoisomer_IB_N_htopoI_like; Topoisomer_IB_N_htopoI_like : N-terminal DNA binding fragment found in eukaryotic DNA topoisomerase (topo) IB proteins similar to the monomeric yeast and human topo I. Topo I enzymes are divided into: topo type IA (bacterial) and type IB (eukaryotic). ...
      pfam01028
      Location:264 → 466
      Topoisom_I; Eukaryotic DNA topoisomerase I, catalytic core
      pfam14370
      Location:512 → 579
      Topo_C_assoc; C-terminal topoisomerase domain

    5. NM_001298338.1 → NP_001285267.1  topoisomerase 1, isoform E [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001285267.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      C6TPA7, X2JKD2
      Conserved Domains (4) summary
      smart00435
      Location:204 → 565
      TOPEUc; DNA Topoisomerase I (eukaryota)
      cd03488
      Location:60 → 274
      Topoisomer_IB_N_htopoI_like; Topoisomer_IB_N_htopoI_like : N-terminal DNA binding fragment found in eukaryotic DNA topoisomerase (topo) IB proteins similar to the monomeric yeast and human topo I. Topo I enzymes are divided into: topo type IA (bacterial) and type IB (eukaryotic). ...
      pfam01028
      Location:276 → 478
      Topoisom_I; Eukaryotic DNA topoisomerase I, catalytic core
      pfam14370
      Location:524 → 591
      Topo_C_assoc; C-terminal topoisomerase domain
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    M9NDX3 M9NDX3
    P30189.1 P30189
    Q494M1 Q494M1
    Q8IR39 Q8IR39
    X2JKD2 X2JKD2

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