Spib Spi-B transcription factor (Spi-1/PU.1 related) [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Spibprovided by MGI
Official Full Name: Spi-B transcription factor (Spi-1/PU.1 related)provided by MGI
Primary source: MGI:MGI:892986
See related: Ensembl:ENSMUSG00000008193 AllianceGenome:MGI:892986
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Spi-B
Summary: Enables DNA-binding transcription factor activity. Involved in immature B cell differentiation. Acts upstream of or within macrophage differentiation and regulation of DNA-templated transcription. Predicted to be part of transcription regulator complex. Predicted to be active in nucleus. Is expressed in brain and liver. Orthologous to human SPIB (Spi-B transcription factor). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in spleen adult (RPKM 101.9), mammary gland adult (RPKM 21.1) and 2 other tissues See more
Orthologs: human all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: 7 B3; 7 28.83 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (44175419..44181511, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (44525995..44532087, complement)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help

See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

SpiB regulates the expression of B-cell-related genes and increases the longevity of memory B cells.
Title: SpiB regulates the expression of B-cell-related genes and increases the longevity of memory B cells.
Opposing Roles for the Related ETS-Family Transcription Factors Spi-B and Spi-C in Regulating B Cell Differentiation and Function.
Title: Opposing Roles for the Related ETS-Family Transcription Factors Spi-B and Spi-C in Regulating B Cell Differentiation and Function.
The mechanism of action of Spi-B in the transcriptional activation of the interferon-alpha4 gene.
Title: The mechanism of action of Spi-B in the transcriptional activation of the interferon-α4 gene.
Mice lacking both PU.1 and SpiB in mature B cells do not generate germinal centers and high-affinity antibody after protein immunization. PU.1 and SpiB double-deficient B cells have a survival defect after engagement of CD40 or Toll-like receptors (TLR), despite paradoxically enhanced plasma cell differentiation.
Title: Environmental sensing by mature B cells is controlled by the transcription factors PU.1 and SpiB.
Mechanistic analysis indicated that E47 activated expression of the transcription factor Spi-B and the suppressor of cytokine signaling 3 (SOCS3), which both downregulated Foxp3 expression. These findings demonstrate that the balance of Id3 and E47 controls the maintenance of Foxp3 expression in Treg cells and, thus, contributes to Treg cell plasticity.
Title: Id3 Maintains Foxp3 Expression in Regulatory T Cells by Controlling a Transcriptional Network of E47, Spi-B, and SOCS3.
Our results suggest that complementary biological functions of PU.1 and Spi-B may be explained by their interaction with a similar set of regions in the genome of B cells.
Title: Genome-wide comparison of PU.1 and Spi-B binding sites in a mouse B lymphoma cell line.
Nfkb1 transcriptional activation by PU.1 and Spi-B promotes toll-like receptor-mediated B cell proliferation.
Title: Nfkb1 activation by the E26 transformation-specific transcription factors PU.1 and Spi-B promotes Toll-like receptor-mediated splenic B cell proliferation.
Data show that transcription factor Spi-B-mediated negative feedback regulation limited medullary thymic epithelial cells (mTECs) development.
Title: Limitation of immune tolerance-inducing thymic epithelial cell development by Spi-B-mediated negative feedback regulation.
there is a small M-cell population with developmental regulation that is Spi-B independent; however, Spi-B is probably a candidate master regulator of M-cell functional maturation and development by another pathway.
Title: Transcription factor Spi-B-dependent and -independent pathways for the development of Peyer's patch M cells.
Data indicate that the developmental defects of plasmacytoid dendritic cells (pDC) in Spi-B-deficient mice were more prominent in the bone marrow.
Title: Spi-B is critical for plasmacytoid dendritic cell function and development.

Submit: New GeneRIF Correction See all GeneRIFs (20)

Variation

Go to the top of the page Help

Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Targeted (4) 1 citation

General gene information

Go to the top of the page Help

Markers

D10Mit157.2 (e-PCR), detects polymorphism
- UniSTS:126061

RH118307 (e-PCR)
- UniSTS:160952

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA-binding transcription activator activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info
enables sequence-specific double-stranded DNA binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in cell differentiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in immature B cell differentiation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within macrophage differentiation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of transcription regulator complex	IC Inferred by Curator more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: transcription factor Spi-B

Names: Ets transcription factor Spi-B; Spi-1/PU.1 related

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_019866.1 → NP_063919.1 transcription factor Spi-B

Status: VALIDATED

Source sequence(s)

CAAA01070168

Consensus CDS

CCDS21209.1

UniProtKB/Swiss-Prot

O35906, O35907, O35909, O55199

UniProtKB/TrEMBL

B9EIW0

Related

ENSMUSP00000035539.5, ENSMUST00000035323.6

Conserved Domains (1) summary

smart00413
Location:173 → 261

ETS; erythroblast transformation specific domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

44175419..44181511 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030242628.1 → XP_030098488.1 transcription factor Spi-B isoform X7

UniProtKB/TrEMBL

B9EIW0

Conserved Domains (1) summary

smart00413
Location:155 → 243

ETS; erythroblast transformation specific domain
XM_006540939.3 → XP_006541002.1 transcription factor Spi-B isoform X9
XM_006540933.3 → XP_006540996.1 transcription factor Spi-B isoform X2

UniProtKB/TrEMBL

A0A0X1KG64

Related

ENSMUSP00000096084.4, ENSMUST00000098483.9

Conserved Domains (1) summary

smart00413
Location:191 → 279

ETS; erythroblast transformation specific domain
XM_006540938.4 → XP_006541001.1 transcription factor Spi-B isoform X8
XM_006540934.4 → XP_006540997.1 transcription factor Spi-B isoform X4

UniProtKB/TrEMBL

A0A0X1KG64

Conserved Domains (1) summary

smart00413
Location:174 → 262

ETS; erythroblast transformation specific domain
XM_006540932.5 → XP_006540995.1 transcription factor Spi-B isoform X1

UniProtKB/TrEMBL

A0A0X1KG64

Conserved Domains (1) summary

smart00413
Location:192 → 280

ETS; erythroblast transformation specific domain
XM_030242627.2 → XP_030098487.1 transcription factor Spi-B isoform X6

UniProtKB/TrEMBL

A0A0U1RPG0

Conserved Domains (1) summary

smart00413
Location:156 → 244

ETS; erythroblast transformation specific domain
XM_006540935.5 → XP_006540998.1 transcription factor Spi-B isoform X3

UniProtKB/TrEMBL

A0A0U1RPG0

Related

ENSMUSP00000145968.2, ENSMUST00000205506.2

Conserved Domains (1) summary

smart00413
Location:174 → 262

ETS; erythroblast transformation specific domain
XM_006540936.4 → XP_006540999.1 transcription factor Spi-B isoform X5

UniProtKB/TrEMBL

B9EIW0

Conserved Domains (1) summary

smart00413
Location:164 → 252

ETS; erythroblast transformation specific domain