Prss8 serine protease 8 (prostasin) [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Prss8provided by MGI
Official Full Name: serine protease 8 (prostasin)provided by MGI
Primary source: MGI:MGI:1923810
See related: Ensembl:ENSMUSG00000030800 AllianceGenome:MGI:1923810
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: fr; CAP1; mCAP1; 2410039E18Rik
Summary: Enables sodium channel regulator activity. Involved in transepithelial transport. Acts upstream of or within hair follicle development and positive regulation of sodium ion transport. Located in external side of plasma membrane and membrane raft. Is expressed in several structures, including alimentary system; brain; extraembryonic component; genitourinary system; and sensory organ. Used to study autosomal recessive congenital ichthyosis 4B and congenital secretory sodium diarrhea 3. Orthologous to human PRSS8 (serine protease 8). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in kidney adult (RPKM 285.2), placenta adult (RPKM 115.0) and 10 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 F3; 7 69.84 cM

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (127524889..127529266, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (127925717..127930149, complement)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Kidney-Specific Membrane-Bound Serine Proteases CAP1/Prss8 and CAP3/St14 Affect ENaC Subunit Abundances but Not Its Activity.
Title: Kidney-Specific Membrane-Bound Serine Proteases CAP1/Prss8 and CAP3/St14 Affect ENaC Subunit Abundances but Not Its Activity.
Membrane protease prostasin promotes insulin secretion by regulating the epidermal growth factor receptor pathway.
Title: Membrane protease prostasin promotes insulin secretion by regulating the epidermal growth factor receptor pathway.
Sodium retention in nephrotic syndrome is independent of the activation of the membrane-anchored serine protease prostasin (CAP1/PRSS8) and its enzymatic activity.
Title: Sodium retention in nephrotic syndrome is independent of the activation of the membrane-anchored serine protease prostasin (CAP1/PRSS8) and its enzymatic activity.
Sodium absorption stimulator prostasin (PRSS8) has an anti-inflammatory effect via downregulation of TLR4 signaling in inflammatory bowel disease.
Title: Sodium absorption stimulator prostasin (PRSS8) has an anti-inflammatory effect via downregulation of TLR4 signaling in inflammatory bowel disease.
Liver-Specific Overexpression of Prostasin Attenuates High-Fat Diet-Induced Metabolic Dysregulation in Mice.
Title: Liver-Specific Overexpression of Prostasin Attenuates High-Fat Diet-Induced Metabolic Dysregulation in Mice.
Genetic Prss8 deficiency caused colitis and an inflamed rectum at an early age and intestinal tumors at a late age, with increased intestinal cell proliferation and migration but decreased cell differentiation. Increased PRSS8 inhibited cancer growth and metastasis in nude mice and inhibited cancer cell migration, invasion, colony and tumor sphere formation.PRSS8 targeted Wnt/beta-catenin, stem-cell, and EMT signaling.
Title: PRSS8 suppresses colorectal carcinogenesis and metastasis.
Studies in colonic T84 cell monolayers revealed that barrier disruption by the colitis-associated Th2-type cytokines, IL-4 and IL-13, down-regulates matriptase as well as prostasin through phosphorylation of the transcriptional regulator STAT6
Title: Inflammatory cytokines down-regulate the barrier-protective prostasin-matriptase proteolytic cascade early in experimental colitis.
a proteolysis-dependent function of activated prostasin in hair follicles, dependent on zymogen conversion by matriptase.
Title: Distinct Developmental Functions of Prostasin (CAP1/PRSS8) Zymogen and Activated Prostasin.
The claudin-4-mediated chloride conductance can be regulated endogenously by a protease-channel-activating protease 1 (cap1).
Title: The Cap1-claudin-4 regulatory pathway is important for renal chloride reabsorption and blood pressure regulation.
HAI-1 regulates the activity of activated matriptase, whereas HAI-2 has an essential role in regulating prostasin-dependent matriptase zymogen activation.
Title: The protease inhibitor HAI-2, but not HAI-1, regulates matriptase activation and shedding through prostasin.

Submit: New GeneRIF Correction See all GeneRIFs (29)

Phenotypes

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Associated conditions

Description	Tests
frizzy GeneReviews: Not available

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2) 1 citation
Gene trapped (2)
Spontaneous (1)
Targeted (9) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH126736 (e-PCR)
- UniSTS:208208

Prss8 (e-PCR), detects polymorphism
- UniSTS:531092

AI313909 (e-PCR)
- UniSTS:159695

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables serine-type endopeptidase activity	IEA Inferred from Electronic Annotation more info
enables sodium channel regulator activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within hair follicle development	IMP Inferred from Mutant Phenotype more info
involved_in positive regulation of sodium ion transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of sodium ion transport	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within positive regulation of sodium ion transport	IGI Inferred from Genetic Interaction more info	PubMed
involved_in proteolysis	IEA Inferred from Electronic Annotation more info
involved_in transepithelial transport	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in external side of plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular region	IEA Inferred from Electronic Annotation more info
located_in extracellular space	ISO Inferred from Sequence Orthology more info
located_in membrane raft	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: prostasin

Names: channel activating protease 1; protease, serine 8 (prostasin); serine protease 8

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001407961.1 → NP_001394890.1 prostasin precursor

Status: VALIDATED

Source sequence(s)

AC149222

UniProtKB/TrEMBL

Q99L44

Related

ENSMUSP00000145904.2, ENSMUST00000206124.2
NM_133351.4 → NP_579929.1 prostasin precursor

See identical proteins and their annotated locations for NP_579929.1

Status: VALIDATED

Source sequence(s)

AC149222

Consensus CDS

CCDS40147.1

UniProtKB/TrEMBL

Q8BJV6, Q99L44

Related

ENSMUSP00000032988.9, ENSMUST00000032988.10

Conserved Domains (1) summary

cd00190
Location:45 → 284

Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...