Itsn1 intersectin 1 (SH3 domain protein 1A) [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Itsn1provided by MGI
Official Full Name: intersectin 1 (SH3 domain protein 1A)provided by MGI
Primary source: MGI:MGI:1338069
See related: Ensembl:ENSMUSG00000022957 AllianceGenome:MGI:1338069
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Ese1; Itsn; Ehsh1; Sh3p17
Summary: Enables kinase activator activity. Involved in synaptic vesicle endocytosis. Acts upstream of or within negative regulation of neuron apoptotic process; positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction; and small GTPase-mediated signal transduction. Located in clathrin-coated pit; endocytic vesicle; and lamellipodium. Is active in calyx of Held and presynaptic endocytic zone. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; sensory organ; and skeleton. Orthologous to human ITSN1 (intersectin 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in CNS E18 (RPKM 7.3), CNS E14 (RPKM 6.7) and 28 other tissues See more
Orthologs: human all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: 16 C4; 16 53.28 cM

Exon count:: 45

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	16	NC_000082.7 (91526198..91717479)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	16	NC_000082.6 (91729310..91920591)

Chromosome 16 - NC_000082.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help

See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data show that the ubiquitously expressed short SH3A variant of intersectin 1 interacts with an N-terminal intramolecular sequence that operates synergistically with the C-terminal motif.
Title: Exon Inclusion Modulates Conformational Plasticity and Autoinhibition of the Intersectin 1 SH3A Domain.
SH3 fusion protein pull downs using ITSN1SH3 A reveal diurnally varying binding of VGLUT1 with slightly reduced VGLUT1/dynamin ratios at the beginning of the light (ZT 0) or the dark (ZT 12) period. Phosphorylation increases binding of VGLUT1 but not of dynamin to EnphSH3. In contrast binding of dynamin to ITSN1SH3 A decreases under phosphorylating conditions with no changes in VGLUT1 binding.
Title: VGLUT1 Binding to Endophilin or Intersectin1 and Dynamin Phosphorylation in a Diurnal Context.
component of Reelin signaling that acts predominantly by facilitating the VLDLR-Dab1 axis to direct neuronal migration in the cortex and hippocampus and to augment synaptic plasticity
Title: Intersectin 1 is a component of the Reelin pathway to regulate neuronal migration and synaptic plasticity in the hippocampus.
Immunohistochemistry and histological and morphometric analyses revealed a twofold increase in proliferative endothelial cells and a 1.35-fold increase in proliferative alpha-smooth muscle actin-positive cells in the lungs of ITSN-deficient mice.
Title: Modulation of Intersectin-1s Lung Expression Induces Obliterative Remodeling and Severe Plexiform Arteriopathy in the Murine Pulmonary Vascular Bed.
PRRT2 interacts with the Src homology 3 domain-bearing protein Intersectin 1, an intracellular protein involved in synaptic vesicle cycling.
Title: A Novel Topology of Proline-rich Transmembrane Protein 2 (PRRT2): HINTS FOR AN INTRACELLULAR FUNCTION AT THE SYNAPSE.
Endocytic deficiency caused by ITSN knock-down modified Alk5 endocytic trafficking and enhances its degradation
Title: Endocytic deficiency induced by ITSN-1s knockdown alters the Smad2/3-Erk1/2 signaling balance downstream of Alk5.
It was concluded that miR-194 regulates ROMK channel activity by modulating ITSN1 expression thereby enhancing ITSN1/WNK-dependent endocytosis.
Title: MicroRNA-194 (miR-194) regulates ROMK channel activity by targeting intersectin 1.
ITSN1 enhances Cbl activity, in part, by modulating the interaction of Cbl with Spry2 through recruitment of Shp2 phosphatase to the Cbl-Spry2 complex.
Title: Receptor tyrosine kinase ubiquitylation involves the dynamic regulation of Cbl-Spry2 by intersectin 1 and the Shp2 tyrosine phosphatase.
intersectin is an endocytic scaffold with a role in fast neurotransmitter release, which may be of prime importance for information processing in the brain
Title: Fast neurotransmitter release regulated by the endocytic scaffold intersectin.
This study demonistrated that Itsn1 mutant mice showed severe deficits in Morris water maze and contextual fear memory tasks.
Title: Vertebrate intersectin1 is repurposed to facilitate cortical midline connectivity and higher order cognition.

Submit: New GeneRIF Correction See all GeneRIFs (16)

Variation

Go to the top of the page Help

Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Gene trapped (3) 1 citation
Targeted (2) 1 citation

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Markers

AI839402 (e-PCR)
- UniSTS:164614

Itsn (e-PCR), detects polymorphism
- UniSTS:259096

D16Mit155 (e-PCR), detects polymorphism
- UniSTS:127788

RH124282 (e-PCR)
- UniSTS:161104

RH136123 (e-PCR)
- UniSTS:210118

AA545208 (e-PCR)
- UniSTS:180946

AI848451 (e-PCR)
- UniSTS:180947

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables guanyl-nucleotide exchange factor activity	IEA Inferred from Electronic Annotation more info
enables kinase activator activity	IDA Inferred from Direct Assay more info	PubMed
enables molecular adaptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables molecular adaptor activity	ISO Inferred from Sequence Orthology more info
enables proline-rich region binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within cell-cell signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in clathrin-dependent synaptic vesicle endocytosis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endosomal transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in exocytosis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within negative regulation of neuron apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of neuron apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of caveolin-mediated endocytosis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of dendritic spine development	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of growth hormone secretion	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction	IDA Inferred from Direct Assay more info	PubMed
involved_in protein localization	ISO Inferred from Sequence Orthology more info
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in regulation of modification of postsynaptic actin cytoskeleton	ISO Inferred from Sequence Orthology more info
involved_in regulation of postsynapse organization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within small GTPase-mediated signal transduction	IDA Inferred from Direct Assay more info	PubMed
involved_in synaptic vesicle endocytosis	IDA Inferred from Direct Assay more info	PubMed
involved_in synaptic vesicle endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in apical dendrite	ISO Inferred from Sequence Orthology more info
is_active_in calyx of Held	IDA Inferred from Direct Assay more info	PubMed
located_in clathrin-coated pit	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with clathrin-coated pit	ISO Inferred from Sequence Orthology more info
located_in clathrin-coated pit	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
is_active_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	TAS Traceable Author Statement more info
located_in dendritic spine	ISO Inferred from Sequence Orthology more info
located_in endocytic vesicle	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
is_active_in intracellular vesicle	IBA Inferred from Biological aspect of Ancestor more info
located_in intracellular vesicle	ISO Inferred from Sequence Orthology more info
located_in lamellipodium	IDA Inferred from Direct Assay more info	PubMed
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
located_in nuclear envelope	IEA Inferred from Electronic Annotation more info
located_in nuclear envelope	ISO Inferred from Sequence Orthology more info
located_in nuclear envelope	ISS Inferred from Sequence or Structural Similarity more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in postsynaptic actin cytoskeleton	ISO Inferred from Sequence Orthology more info
is_active_in presynaptic endocytic zone	IDA Inferred from Direct Assay more info	PubMed
is_active_in presynaptic membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in recycling endosome	IEA Inferred from Electronic Annotation more info

General protein information

Go to the top of the page Help

Preferred Names: intersectin-1

Names: EH and SH3 domains protein 1; Eh domain, SH3 domain regulator of endocytosis 1; intersectin (SH3 domain protein 1A); intersectin-L

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001110275.1 → NP_001103745.1 intersectin-1 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) has multiple differences in the presence and absence of exons at its 3' end, compared to variant 1. These differences produce a unique 3' UTR and result in a protein (isoform 2) that is shorter at the C-terminus, compared to isoform 1.

Source sequence(s)

AF132478, BC062938

Consensus CDS

CCDS49913.1

UniProtKB/TrEMBL

E9Q3I5

Related

ENSMUSP00000056011.8, ENSMUST00000056482.14

Conserved Domains (11) summary

smart00027
Location:214 → 309

EH; Eps15 homology domain

cd11987
Location:742 → 796

SH3_Intersectin1_1; First Src homology 3 domain (or SH3A) of Intersectin-1

cd11991
Location:999 → 1050

SH3_Intersectin1_3; Third Src homology 3 domain (or SH3C) of Intersectin-1

cd11995
Location:1151 → 1204

SH3_Intersectin1_5; Fifth Src homology 3 domain (or SH3E) of Intersectin-1

COG1340
Location:350 → 646

COG1340; Uncharacterized coiled-coil protein, contains DUF342 domain [Function unknown]

cd00052
Location:225 → 291

EH; Eps15 homology domain; found in proteins implicated in endocytosis, vesicle transport, and signal transduction. The alignment contains a pair of EF-hand motifs, typically one of them is canonical and binds to Ca2+, while the other may not bind to Ca2+. A ...

pfam16617
Location:796 → 910

INTAP; Intersectin and clathrin adaptor AP2 binding region

cd16269
Location:677 → 686

GBP_C; coiled coil [structural motif]

cl17036
Location:1067 → 1131

SH3; Src Homology 3 domain superfamily

cl20817
Location:615 → 706

GBP_C; Guanylate-binding protein, C-terminal domain

cl23720
Location:546 → 646

RILP-like; Rab interacting lysosomal protein-like 1 and 2 (Rilpl1 and Rilpl2)
NM_001110276.1 → NP_001103746.1 intersectin-1 isoform 3

See identical proteins and their annotated locations for NP_001103746.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) has multiple differences in the presence and absence of exons at its 3' end, compared to variant 1. These differences produce a unique 3' UTR and result in a shorter protein (isoform 3) with a distinct C-terminus, compared to isoform 1.

Source sequence(s)

BC066105, CB519152

Consensus CDS

CCDS49914.1

UniProtKB/TrEMBL

Q3U2A1, Q6NZJ5

Related

ENSMUSP00000093598.4, ENSMUST00000095909.10

Conserved Domains (4) summary

smart00027
Location:214 → 309

EH; Eps15 homology domain

cd00052
Location:225 → 291

EH; Eps15 homology domain; found in proteins implicated in endocytosis, vesicle transport, and signal transduction. The alignment contains a pair of EF-hand motifs, typically one of them is canonical and binds to Ca2+, while the other may not bind to Ca2+. A ...

pfam03957
Location:93 → 190

Jun; Jun-like transcription factor

cl23765
Location:351 → 465

iSH2_PI3K_IA_R; Inter-Src homology 2 (iSH2) helical domain of Class IA Phosphoinositide 3-kinase Regulatory subunits
NM_010587.2 → NP_034717.2 intersectin-1 isoform 1

See identical proteins and their annotated locations for NP_034717.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

Source sequence(s)

AC126053, AF132481, AK082606, BC062938

Consensus CDS

CCDS37402.1

UniProtKB/Swiss-Prot

F8VQE5, Q9R143, Q9Z0R4

Related

ENSMUSP00000109635.3, ENSMUST00000114002.9

Conserved Domains (17) summary

cd08375
Location:1575 → 1709

C2_Intersectin; C2 domain present in Intersectin

smart00027
Location:214 → 309

EH; Eps15 homology domain

cd11987
Location:742 → 796

SH3_Intersectin1_1; First Src homology 3 domain (or SH3A) of Intersectin-1

cd11989
Location:910 → 961

SH3_Intersectin1_2; Second Src homology 3 domain (or SH3B) of Intersectin-1

cd11991
Location:999 → 1050

SH3_Intersectin1_3; Third Src homology 3 domain (or SH3C) of Intersectin-1

cd11993
Location:1067 → 1131

SH3_Intersectin1_4; Fourth Src homology 3 domain (or SH3D) of Intersectin-1

cd11995
Location:1151 → 1204

SH3_Intersectin1_5; Fifth Src homology 3 domain (or SH3E) of Intersectin-1

COG1340
Location:350 → 646

COG1340; Uncharacterized coiled-coil protein, contains DUF342 domain [Function unknown]

cd00052
Location:225 → 291

EH; Eps15 homology domain; found in proteins implicated in endocytosis, vesicle transport, and signal transduction. The alignment contains a pair of EF-hand motifs, typically one of them is canonical and binds to Ca2+, while the other may not bind to Ca2+. A ...

cd13264
Location:1441 → 1572

PH_ITSN; Intersectin Pleckstrin homology (PH) domain

pfam00169
Location:1458 → 1564

PH; PH domain

pfam00621
Location:1235 → 1415

RhoGEF; RhoGEF domain

pfam03957
Location:93 → 190

Jun; Jun-like transcription factor

pfam16617
Location:796 → 910

INTAP; Intersectin and clathrin adaptor AP2 binding region

cd16269
Location:677 → 686

GBP_C; coiled coil [structural motif]

cl20817
Location:615 → 706

GBP_C; Guanylate-binding protein, C-terminal domain

cl23720
Location:546 → 646

RILP-like; Rab interacting lysosomal protein-like 1 and 2 (Rilpl1 and Rilpl2)

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000082.7 Reference GRCm39 C57BL/6J

Range

91526198..91717479

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006522934.4 → XP_006522997.1 intersectin-1 isoform X4

UniProtKB/TrEMBL

E9Q0N0

Related

ENSMUSP00000066361.6, ENSMUST00000064797.12

Conserved Domains (12) summary

PTZ00121
Location:331 → 715

PTZ00121; MAEBL; Provisional

cd08375
Location:1580 → 1714

C2_Intersectin; C2 domain present in Intersectin

PRK14959
Location:42 → 184

PRK14959; DNA polymerase III subunits gamma and tau; Provisional

smart00027
Location:214 → 309

EH; Eps15 homology domain

cd11989
Location:915 → 966

SH3_Intersectin1_2; Second Src homology 3 domain (or SH3B) of Intersectin-1

cd11991
Location:1004 → 1055

SH3_Intersectin1_3; Third Src homology 3 domain (or SH3C) of Intersectin-1

cd11993
Location:1072 → 1136

SH3_Intersectin1_4; Fourth Src homology 3 domain (or SH3D) of Intersectin-1

cd11995
Location:1156 → 1209

SH3_Intersectin1_5; Fifth Src homology 3 domain (or SH3E) of Intersectin-1

cd00160
Location:1236 → 1419

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

pfam16617
Location:801 → 915

INTAP; Intersectin and clathrin adaptor AP2 binding region

pfam16652
Location:1438 → 1591

PH_13; Pleckstrin homology domain

cl17036
Location:742 → 801

SH3; Src Homology 3 domain superfamily
XM_036159794.1 → XP_036015687.1 intersectin-1 isoform X11

UniProtKB/TrEMBL

E9Q3I8, E9Q3I9

Related

ENSMUSP00000109629.2, ENSMUST00000113996.8

Conserved Domains (7) summary

PTZ00121
Location:331 → 715

PTZ00121; MAEBL; Provisional

PRK14959
Location:42 → 184

PRK14959; DNA polymerase III subunits gamma and tau; Provisional

smart00027
Location:214 → 309

EH; Eps15 homology domain

cd11987
Location:742 → 796

SH3_Intersectin1_1; First Src homology 3 domain (or SH3A) of Intersectin-1

cd11995
Location:1080 → 1133

SH3_Intersectin1_5; Fifth Src homology 3 domain (or SH3E) of Intersectin-1

pfam16617
Location:796 → 910

INTAP; Intersectin and clathrin adaptor AP2 binding region

cl17036
Location:999 → 1060

SH3; Src Homology 3 domain superfamily
XM_006522931.3 → XP_006522994.1 intersectin-1 isoform X1

Conserved Domains (12) summary

PTZ00121
Location:355 → 739

PTZ00121; MAEBL; Provisional

cd08375
Location:1604 → 1738

C2_Intersectin; C2 domain present in Intersectin

smart00027
Location:238 → 333

EH; Eps15 homology domain

cd11989
Location:939 → 990

SH3_Intersectin1_2; Second Src homology 3 domain (or SH3B) of Intersectin-1

cd11991
Location:1028 → 1079

SH3_Intersectin1_3; Third Src homology 3 domain (or SH3C) of Intersectin-1

cd11993
Location:1096 → 1160

SH3_Intersectin1_4; Fourth Src homology 3 domain (or SH3D) of Intersectin-1

cd11995
Location:1180 → 1233

SH3_Intersectin1_5; Fifth Src homology 3 domain (or SH3E) of Intersectin-1

PLN02983
Location:155 → 226

PLN02983; biotin carboxyl carrier protein of acetyl-CoA carboxylase

cd00160
Location:1260 → 1443

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

pfam16617
Location:825 → 939

INTAP; Intersectin and clathrin adaptor AP2 binding region

pfam16652
Location:1462 → 1615

PH_13; Pleckstrin homology domain

cl17036
Location:766 → 825

SH3; Src Homology 3 domain superfamily
XM_006522935.2 → XP_006522998.1 intersectin-1 isoform X5

Conserved Domains (11) summary

PTZ00121
Location:355 → 739

PTZ00121; MAEBL; Provisional

cd08375
Location:1533 → 1667

C2_Intersectin; C2 domain present in Intersectin

PRK14959
Location:66 → 208

PRK14959; DNA polymerase III subunits gamma and tau; Provisional

smart00027
Location:238 → 333

EH; Eps15 homology domain

cd11987
Location:766 → 825

SH3_Intersectin1_1; First Src homology 3 domain (or SH3A) of Intersectin-1

cd11989
Location:939 → 990

SH3_Intersectin1_2; Second Src homology 3 domain (or SH3B) of Intersectin-1

cd11995
Location:1109 → 1162

SH3_Intersectin1_5; Fifth Src homology 3 domain (or SH3E) of Intersectin-1

cd00160
Location:1189 → 1372

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

pfam16617
Location:825 → 939

INTAP; Intersectin and clathrin adaptor AP2 binding region

pfam16652
Location:1391 → 1544

PH_13; Pleckstrin homology domain

cl17036
Location:1028 → 1089

SH3; Src Homology 3 domain superfamily
XM_006522933.2 → XP_006522996.1 intersectin-1 isoform X3

Conserved Domains (12) summary

PTZ00121
Location:355 → 739

PTZ00121; MAEBL; Provisional

cd08375
Location:1599 → 1733

C2_Intersectin; C2 domain present in Intersectin

smart00027
Location:238 → 333

EH; Eps15 homology domain

cd11987
Location:766 → 820

SH3_Intersectin1_1; First Src homology 3 domain (or SH3A) of Intersectin-1

cd11989
Location:934 → 985

SH3_Intersectin1_2; Second Src homology 3 domain (or SH3B) of Intersectin-1

cd11991
Location:1023 → 1074

SH3_Intersectin1_3; Third Src homology 3 domain (or SH3C) of Intersectin-1

cd11993
Location:1091 → 1155

SH3_Intersectin1_4; Fourth Src homology 3 domain (or SH3D) of Intersectin-1

cd11995
Location:1175 → 1228

SH3_Intersectin1_5; Fifth Src homology 3 domain (or SH3E) of Intersectin-1

PLN02983
Location:155 → 226

PLN02983; biotin carboxyl carrier protein of acetyl-CoA carboxylase

cd00160
Location:1255 → 1438

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

pfam16617
Location:820 → 934

INTAP; Intersectin and clathrin adaptor AP2 binding region

pfam16652
Location:1457 → 1610

PH_13; Pleckstrin homology domain
XM_006522936.2 → XP_006522999.1 intersectin-1 isoform X6

Conserved Domains (11) summary

PTZ00121
Location:355 → 739

PTZ00121; MAEBL; Provisional

cd08375
Location:1528 → 1662

C2_Intersectin; C2 domain present in Intersectin

PRK14959
Location:66 → 208

PRK14959; DNA polymerase III subunits gamma and tau; Provisional

smart00027
Location:238 → 333

EH; Eps15 homology domain

cd11987
Location:766 → 820

SH3_Intersectin1_1; First Src homology 3 domain (or SH3A) of Intersectin-1

cd11989
Location:934 → 985

SH3_Intersectin1_2; Second Src homology 3 domain (or SH3B) of Intersectin-1

cd11995
Location:1104 → 1157

SH3_Intersectin1_5; Fifth Src homology 3 domain (or SH3E) of Intersectin-1

cd00160
Location:1184 → 1367

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

pfam16617
Location:820 → 934

INTAP; Intersectin and clathrin adaptor AP2 binding region

pfam16652
Location:1386 → 1539

PH_13; Pleckstrin homology domain

cl17036
Location:1023 → 1084

SH3; Src Homology 3 domain superfamily
XM_006522938.2 → XP_006523001.1 intersectin-1 isoform X8

UniProtKB/TrEMBL

E9Q3I5

Conserved Domains (7) summary

PTZ00121
Location:355 → 739

PTZ00121; MAEBL; Provisional

smart00027
Location:238 → 333

EH; Eps15 homology domain

cd11991
Location:1028 → 1079

SH3_Intersectin1_3; Third Src homology 3 domain (or SH3C) of Intersectin-1

cd11995
Location:1180 → 1233

SH3_Intersectin1_5; Fifth Src homology 3 domain (or SH3E) of Intersectin-1

PLN02983
Location:155 → 226

PLN02983; biotin carboxyl carrier protein of acetyl-CoA carboxylase

pfam16617
Location:825 → 939

INTAP; Intersectin and clathrin adaptor AP2 binding region

cl17036
Location:1096 → 1160

SH3; Src Homology 3 domain superfamily
XM_036159793.1 → XP_036015686.1 intersectin-1 isoform X10

UniProtKB/TrEMBL

E9Q3I9

Conserved Domains (6) summary

PTZ00121
Location:355 → 739

PTZ00121; MAEBL; Provisional

smart00027
Location:238 → 333

EH; Eps15 homology domain

cd11995
Location:1109 → 1162

SH3_Intersectin1_5; Fifth Src homology 3 domain (or SH3E) of Intersectin-1

PLN02983
Location:155 → 226

PLN02983; biotin carboxyl carrier protein of acetyl-CoA carboxylase

pfam16617
Location:825 → 939

INTAP; Intersectin and clathrin adaptor AP2 binding region

cl17036
Location:1028 → 1089

SH3; Src Homology 3 domain superfamily
XM_006522939.2 → XP_006523002.1 intersectin-1 isoform X9

UniProtKB/TrEMBL

E9Q3I5

Conserved Domains (8) summary

PTZ00121
Location:355 → 739

PTZ00121; MAEBL; Provisional

smart00027
Location:238 → 333

EH; Eps15 homology domain

cd11987
Location:766 → 820

SH3_Intersectin1_1; First Src homology 3 domain (or SH3A) of Intersectin-1

cd11991
Location:1023 → 1074

SH3_Intersectin1_3; Third Src homology 3 domain (or SH3C) of Intersectin-1

cd11995
Location:1175 → 1228

SH3_Intersectin1_5; Fifth Src homology 3 domain (or SH3E) of Intersectin-1

PLN02983
Location:155 → 226

PLN02983; biotin carboxyl carrier protein of acetyl-CoA carboxylase

pfam16617
Location:820 → 934

INTAP; Intersectin and clathrin adaptor AP2 binding region

cl17036
Location:1091 → 1155

SH3; Src Homology 3 domain superfamily
XM_006522940.2 → XP_006523003.1 intersectin-1 isoform X12

Conserved Domains (5) summary

PTZ00121
Location:355 → 739

PTZ00121; MAEBL; Provisional

smart00027
Location:238 → 333

EH; Eps15 homology domain

PLN02983
Location:155 → 226

PLN02983; biotin carboxyl carrier protein of acetyl-CoA carboxylase

pfam16617
Location:825 → 931

INTAP; Intersectin and clathrin adaptor AP2 binding region

cl17036
Location:766 → 825

SH3; Src Homology 3 domain superfamily
XM_036159795.1 → XP_036015688.1 intersectin-1 isoform X13

Conserved Domains (5) summary

PTZ00121
Location:355 → 739

PTZ00121; MAEBL; Provisional

smart00027
Location:238 → 333

EH; Eps15 homology domain

cd11987
Location:766 → 820

SH3_Intersectin1_1; First Src homology 3 domain (or SH3A) of Intersectin-1

pfam16617
Location:820 → 926

INTAP; Intersectin and clathrin adaptor AP2 binding region

NF033761
Location:117 → 245

gliding_GltJ; adventurous gliding motility protein GltJ
XM_006522932.5 → XP_006522995.1 intersectin-1 isoform X2

Conserved Domains (12) summary

PTZ00121
Location:353 → 737

PTZ00121; MAEBL; Provisional

cd08375
Location:1602 → 1736

C2_Intersectin; C2 domain present in Intersectin

PRK14959
Location:64 → 206

PRK14959; DNA polymerase III subunits gamma and tau; Provisional

smart00027
Location:236 → 331

EH; Eps15 homology domain

cd11989
Location:937 → 988

SH3_Intersectin1_2; Second Src homology 3 domain (or SH3B) of Intersectin-1

cd11991
Location:1026 → 1077

SH3_Intersectin1_3; Third Src homology 3 domain (or SH3C) of Intersectin-1

cd11993
Location:1094 → 1158

SH3_Intersectin1_4; Fourth Src homology 3 domain (or SH3D) of Intersectin-1

cd11995
Location:1178 → 1231

SH3_Intersectin1_5; Fifth Src homology 3 domain (or SH3E) of Intersectin-1

cd00160
Location:1258 → 1441

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

pfam16617
Location:823 → 937

INTAP; Intersectin and clathrin adaptor AP2 binding region

pfam16652
Location:1460 → 1613

PH_13; Pleckstrin homology domain

cl17036
Location:764 → 823

SH3; Src Homology 3 domain superfamily
XM_006522937.4 → XP_006523000.1 intersectin-1 isoform X7

Conserved Domains (10) summary

PTZ00121
Location:138 → 522

PTZ00121; MAEBL; Provisional

cd08375
Location:1387 → 1521

C2_Intersectin; C2 domain present in Intersectin

smart00027
Location:21 → 116

EH; Eps15 homology domain

cd11991
Location:811 → 862

SH3_Intersectin1_3; Third Src homology 3 domain (or SH3C) of Intersectin-1

cd11993
Location:879 → 943

SH3_Intersectin1_4; Fourth Src homology 3 domain (or SH3D) of Intersectin-1

cd11995
Location:963 → 1016

SH3_Intersectin1_5; Fifth Src homology 3 domain (or SH3E) of Intersectin-1

cd00160
Location:1043 → 1226

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

pfam16617
Location:608 → 722

INTAP; Intersectin and clathrin adaptor AP2 binding region

pfam16652
Location:1245 → 1398

PH_13; Pleckstrin homology domain

cl17036
Location:549 → 608

SH3; Src Homology 3 domain superfamily