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    TppII Tripeptidyl-peptidase II [ Drosophila melanogaster (fruit fly) ]

    Gene ID: 36444, updated on 9-Dec-2024

    Summary

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    Official Symbol
    TppIIprovided by FlyBase
    Official Full Name
    Tripeptidyl-peptidase IIprovided by FlyBase
    Primary source
    FLYBASE:FBgn0020370
    Locus tag
    Dmel_CG3991
    See related
    AllianceGenome:FB:FBgn0020370
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Drosophila melanogaster
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
    Also known as
    anon-WO0118547.127; CG3991; Dmel\CG3991; dTPP II; dTPPII; l(2)k14301; TPP II; TPP2; TPPII
    Summary
    Enables identical protein binding activity and tripeptidyl-peptidase activity. Involved in proteolysis. Located in cytosol. Part of protein-containing complex. Is expressed in several structures, including Malpighian tubule primordium; adult head; epithelium; gland; and gut section. Human ortholog(s) of this gene implicated in primary immunodeficiency disease. Orthologous to human TPP2 (tripeptidyl peptidase 2). [provided by Alliance of Genome Resources, Dec 2024]
    Orthologs
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    Genomic context

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    See TppII in Genome Data Viewer
    Location:
    49F4-49F4; 2-68 cM
    Exon count:
    15
    Annotation release Status Assembly Chr Location
    Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 2R NT_033778.4 (13152885..13159141, complement)
    Release 5.57 previous assembly Release 5 (GCF_000001215.2) 2R NT_033778.3 (9040390..9046646, complement)

    Chromosome 2R - NT_033778.4Genomic Context describing neighboring genes Neighboring gene Chromosome associated protein G Neighboring gene Activated Cdc42 kinase-like Neighboring gene NADH dehydrogenase (ubiquinone) MWFE subunit Neighboring gene Neurospecific receptor kinase Neighboring gene uncharacterized protein Neighboring gene Glial Lazarillo

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. A giant protease with a twist: the TPP II complex from Drosophila studied by electron microscopy. Rockel B, et al. EMBO J, 2002 Nov 15. PMID 12426370, Free PMC Article
    2. Molecular architecture and assembly mechanism of Drosophila tripeptidyl peptidase II. Rockel B, et al. Proc Natl Acad Sci U S A, 2005 Jul 19. PMID 16006508, Free PMC Article
    3. Size matters for the tripeptidylpeptidase II complex from Drosophila: The 6-MDa spindle form stabilizes the activated state. Seyit G, et al. J Biol Chem, 2006 Sep 1. PMID 16799156
    4. Hybrid molecular structure of the giant protease tripeptidyl peptidase II. Chuang CK, et al. Nat Struct Mol Biol, 2010 Aug. PMID 20676100, Free PMC Article
    5. Molecular ruler of tripeptidylpeptidase II: mechanistic principle of exopeptidase selectivity. Peters J, et al. Biochem Biophys Res Commun, 2011 Oct 14. PMID 21946061

    See all (50) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Previously unknown differences between TPP II orthologues and subtilisin as well as features that might be conserved within the entire family of subtilisin-like serine peptidases.
      Title: Exploring the active site of tripeptidyl-peptidase II through studies of pH dependence of reaction kinetics.
    2. a systematic kinetic analysis of wild type Drosophila melanogaster TPPII and five point mutants of the double-Glu-motif (E312/E343) involving natural substrates were performed.
      Title: Molecular ruler of tripeptidylpeptidase II: mechanistic principle of exopeptidase selectivity.
    3. The structure suggests a model for activation of TPP II involving the relocation of a flexible loop and a repositioning of the active-site serine, coupling it to holocomplex assembly and active-site sequestration.
      Title: Hybrid molecular structure of the giant protease tripeptidyl peptidase II.
    4. the unique spindle form of the holo-complex of TPP II represents an assembly motif stabilizing a highly active state
      Title: Size matters for the tripeptidylpeptidase II complex from Drosophila: The 6-MDa spindle form stabilizes the activated state.
    5. The 150 kDa subunits of TPPII form a superstructure composed of 2 segmented & twisted strands. Each strand is 12.5 nm in width & composed of 11 segments that enclose a central channel.
      Title: A giant protease with a twist: the TPP II complex from Drosophila studied by electron microscopy.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    mobile_element

    • Loc: c13156015-13155983 mobile_element_type = transposon:INE-1{}5010

    Gene Ontology Provided by FlyBase

    Function Evidence Code Pubs
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables serine hydrolase activity HDA PubMed 
    enables serine-type endopeptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables tripeptidyl-peptidase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables tripeptidyl-peptidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables tripeptidyl-peptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in proteolysis IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in proteolysis IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of protein-containing complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    Tripeptidyl-peptidase II
    Names
    CG3991-PA
    CG3991-PD
    CG3991-PE
    CG3991-PF
    TppII-PA
    TppII-PD
    TppII-PE
    TppII-PF
    tripeptidyl peptidase II
    tripeptidyl-peptidase II
    tripeptidylpeptidase II
    NP_001286376.1
    NP_001286377.1
    NP_477247.1
    NP_725252.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NT_033778.4 Reference assembly

      Range
      13152885..13159141 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_165966.2 → NP_725252.1  Tripeptidyl-peptidase II, isoform D [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_725252.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      B5RJ14, O76251, Q8IH59, Q8ML58, Q9V6K1
      Related
      FBpp0086887
      Conserved Domains (4) summary
      cd04857
      Location:101 → 589
      Peptidases_S8_Tripeptidyl_Aminopeptidase_II; Peptidase S8 family domain in Tripeptidyl aminopeptidases_II
      pfam00082
      Location:331 → 600
      Peptidase_S8; Subtilase family
      pfam12580
      Location:892 → 1073
      TPPII; Tripeptidyl peptidase II
      pfam12583
      Location:1144 → 1257
      TPPII_N; Tripeptidyl peptidase II N terminal

    2. NM_001299447.1 → NP_001286376.1  Tripeptidyl-peptidase II, isoform F [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001286376.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A0B4LGC0
      Conserved Domains (4) summary
      cd04857
      Location:14 → 502
      Peptidases_S8_Tripeptidyl_Aminopeptidase_II; Peptidase S8 family domain in Tripeptidyl aminopeptidases_II
      pfam00082
      Location:244 → 513
      Peptidase_S8; Subtilase family
      pfam12580
      Location:805 → 986
      TPPII; Tripeptidyl peptidase II
      pfam12583
      Location:1057 → 1170
      TPPII_N; Tripeptidyl peptidase II N terminal

    3. NM_057899.3 → NP_477247.1  Tripeptidyl-peptidase II, isoform A [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_477247.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A0B4LGC0
      Related
      FBpp0086888
      Conserved Domains (4) summary
      cd04857
      Location:14 → 502
      Peptidases_S8_Tripeptidyl_Aminopeptidase_II; Peptidase S8 family domain in Tripeptidyl aminopeptidases_II
      pfam00082
      Location:244 → 513
      Peptidase_S8; Subtilase family
      pfam12580
      Location:805 → 986
      TPPII; Tripeptidyl peptidase II
      pfam12583
      Location:1057 → 1170
      TPPII_N; Tripeptidyl peptidase II N terminal

    4. NM_001299448.1 → NP_001286377.1  Tripeptidyl-peptidase II, isoform E [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001286377.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A0B4LF88
      Conserved Domains (4) summary
      pfam00082
      Location:244 → 534
      Peptidase_S8; Subtilase family
      pfam12580
      Location:826 → 1007
      TPPII; Tripeptidyl peptidase II
      pfam12583
      Location:1078 → 1191
      TPPII_N; Tripeptidyl peptidase II N terminal
      cl10459
      Location:14 → 523
      Peptidases_S8_S53; Peptidase domain in the S8 and S53 families
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    A0A0B4LF88 A0A0B4LF88
    A0A0B4LGC0 A0A0B4LGC0
    Q9V6K1.2 Q9V6K1

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