Iswi Imitation SWI [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Iswiprovided by FlyBase
Official Full Name: Imitation SWIprovided by FlyBase
Primary source: FLYBASE:FBgn0011604
Locus tag: Dmel_CG8625
See related: AllianceGenome:FB:FBgn0011604
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: ACF; anon-EP1279744.124; CG8625; CHRAC; dCHRAC; dISWI; Dmel\CG8625; dNURF; ISW; iswi; ISWI; NURF; NURF 140; Nurf-140; NURF-140; p140; p140/ISWI; SWI
Summary: Enables ATP hydrolysis activity; DNA-binding transcription factor binding activity; and nucleosome array spacer activity. Involved in several processes, including DNA replication-dependent chromatin assembly; nucleus organization; and regulation of gene expression. Located in chromatin; nucleus; and polytene chromosome. Part of ISWI-type complex and transcription regulator complex. Is expressed in several structures, including embryonic/larval midgut primordium; ganglia; germline cell; head epidermis primordium; and somatic cell of testis. Used to study central nervous system disease. Orthologous to several human genes including SMARCA1 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 49B10-49B10; 2-67 cM

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (12636671..12640628)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (8524176..8528133)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Here, the authors show that the Chd1 remodeler from Saccharomyces cerevisiae and ISWI remodelers from human and Drosophila have distinct spatial requirements for the acidic patch. Unlike Chd1, which is equally affected by entry- and exit-side mutations, ISWI remodelers strongly depend on the entry-side acidic patch.
Title: Asymmetry between the two acidic patches dictates the direction of nucleosome sliding by the ISWI chromatin remodeler.
Data show that SWI/SNF complexes play a role in the cleavage of specific pre-mRNAs in animal cells.
Title: SWI/SNF interacts with cleavage and polyadenylation factors and facilitates pre-mRNA 3' end processing.
the role of ISWI in the hnRNPshsromega interaction
Title: ISWI ATP-dependent remodeling of nucleoplasmic ω-speckles in the brain of Drosophila melanogaster.
ISWI enables bi-directional transitions between two fundamentally different forms of chromatin.
Title: A Testis-Specific Chaperone and the Chromatin Remodeler ISWI Mediate Repackaging of the Paternal Genome.
Insertion of flexible linkers at strategic sites in ISWI that disrupt a power stroke does not affect nucleosome sliding.
Title: No need for a power stroke in ISWI-mediated nucleosome sliding.
In a purified system ISWI- and CHD1-type nucleosome remodelers have a clamping activity such that they not only generate regularly spaced nucleosome arrays but also generate constant spacing regardless of nucleosome density.
Title: Nucleosome spacing generated by ISWI and CHD1 remodelers is constant regardless of nucleosome density.
The effect of H4 tail at lysine 16 acetylation on ISWI remodelling depends on the precise nature of the substrate.
Title: ISWI remodelling of physiological chromatin fibres acetylated at lysine 16 of histone H4.
finding show all fundamental aspects of nucleosome-remodeling catalysis are contained within the compact ATPase domain of ISWI
Title: The ATPase domain of ISWI is an autonomous nucleosome remodeling machine.
define two new, conserved and separate regulatory regions on Drosophila ISWI, termed AutoN and NegC, which negatively regulate ATP hydrolysis (AutoN) or the coupling of ATP hydrolysis to productive DNA translocation (NegC).
Title: Regulation of ISWI involves inhibitory modules antagonized by nucleosomal epitopes.
This study revealed a novel conformation of an ISWI ATPase domain
Title: Probing the conformation of the ISWI ATPase domain with genetically encoded photoreactive crosslinkers and mass spectrometry.

Submit: New GeneRIF Correction See all GeneRIFs (26)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP hydrolysis activity	ISM Inferred from Sequence Model more info	PubMed
enables ATP-dependent activity, acting on DNA	IDA Inferred from Direct Assay more info	PubMed
enables ATP-dependent activity, acting on DNA	IGI Inferred from Genetic Interaction more info	PubMed
enables ATP-dependent chromatin remodeler activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP-dependent chromatin remodeler activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent chromatin remodeler activity	ISM Inferred from Sequence Model more info	PubMed
enables DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables chromatin binding	IBA Inferred from Biological aspect of Ancestor more info
enables histone octamer slider activity	IDA Inferred from Direct Assay more info	PubMed
enables nucleosome array spacer activity	IBA Inferred from Biological aspect of Ancestor more info
enables nucleosome array spacer activity	IDA Inferred from Direct Assay more info	PubMed
enables nucleosome binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	TAS Traceable Author Statement more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA replication-dependent chromatin assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in chromatin organization	IDA Inferred from Direct Assay more info	PubMed
involved_in chromatin organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin remodeling	IDA Inferred from Direct Assay more info	PubMed
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in chromatin remodeling	TAS Traceable Author Statement more info	PubMed
involved_in heterochromatin formation	IBA Inferred from Biological aspect of Ancestor more info
involved_in heterochromatin formation	IPI Inferred from Physical Interaction more info	PubMed
involved_in negative regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear speck organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleosome organization	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of DNA-templated transcription	TAS Traceable Author Statement more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of circadian rhythm	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sperm DNA condensation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sperm DNA decondensation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of ACF complex	IDA Inferred from Direct Assay more info	PubMed
part_of ACF complex	NAS Non-traceable Author Statement more info	PubMed
part_of ACF complex	TAS Traceable Author Statement more info	PubMed
part_of CHRAC	IDA Inferred from Direct Assay more info	PubMed
part_of CHRAC	NAS Non-traceable Author Statement more info	PubMed
part_of CHRAC	TAS Traceable Author Statement more info	PubMed
part_of ISWI-type complex	IDA Inferred from Direct Assay more info	PubMed
part_of NURF complex	IDA Inferred from Direct Assay more info	PubMed
part_of NURF complex	TAS Traceable Author Statement more info	PubMed
part_of RSF complex	IPI Inferred from Physical Interaction more info	PubMed
located_in chromatin	IBA Inferred from Biological aspect of Ancestor more info
located_in chromatin	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
colocalizes_with polytene chromosome	IDA Inferred from Direct Assay more info	PubMed
located_in polytene chromosome	IDA Inferred from Direct Assay more info	PubMed
part_of transcription regulator complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: imitation SWI

Names: CG8625-PA; CG8625-PB; CG8625-PC; ISWI; ISWI ATPase; Iswi-PA; Iswi-PB; Iswi-PC; imitation SW; imitation switch; imitation-SWI; nucleosome remodeling factor; nucleosome remodeling factor - 140kD

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

12636671..12640628

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_078995.2 → NP_523719.1 imitation SWI, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_523719.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A4UZF0, B5X0J3, Q0E9A4, Q24368, Q8SX14, Q9V6E8

UniProtKB/TrEMBL

I1V4Y8

Related

FBpp0086954

Conserved Domains (8) summary

smart00717
Location:797 → 838

SANT; SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains

cd00046
Location:148 → 287

DEXDc; DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.

cd00079
Location:422 → 553

HELICc; Helicase superfamily c-terminal domain; associated with DEXDc-, DEAD-, and DEAH-box proteins, yeast initiation factor 4A, Ski2p, and Hepatitis C virus NS3 helicases; this domain is found in a wide variety of helicases and helicase related proteins; may ...

cd00167
Location:799 → 838

SANT; 'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains. Tandem copies of the domain bind telomeric DNA tandem repeatsas part of the capping complex. Binding is sequence dependent for repeats which contain the G/C rich motif [C2-3 A (CA)1-6]. The domain is ...

pfam00176
Location:131 → 411

SNF2_N; SNF2 family N-terminal domain

pfam09110
Location:697 → 796

HAND; HAND

pfam09111
Location:853 → 965

SLIDE; SLIDE

pfam13892
Location:30 → 67

DBINO; DNA-binding domain
NM_165931.2 → NP_725204.1 imitation SWI, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_725204.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A4UZF0, B5X0J3, Q0E9A4, Q24368, Q8SX14, Q9V6E8

UniProtKB/TrEMBL

I1V4Y8

Related

FBpp0086956

Conserved Domains (8) summary

smart00717
Location:797 → 838

SANT; SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains

cd00046
Location:148 → 287

DEXDc; DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.

cd00079
Location:422 → 553

HELICc; Helicase superfamily c-terminal domain; associated with DEXDc-, DEAD-, and DEAH-box proteins, yeast initiation factor 4A, Ski2p, and Hepatitis C virus NS3 helicases; this domain is found in a wide variety of helicases and helicase related proteins; may ...

cd00167
Location:799 → 838

SANT; 'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains. Tandem copies of the domain bind telomeric DNA tandem repeatsas part of the capping complex. Binding is sequence dependent for repeats which contain the G/C rich motif [C2-3 A (CA)1-6]. The domain is ...

pfam00176
Location:131 → 411

SNF2_N; SNF2 family N-terminal domain

pfam09110
Location:697 → 796

HAND; HAND

pfam09111
Location:853 → 965

SLIDE; SLIDE

pfam13892
Location:30 → 67

DBINO; DNA-binding domain
NM_165930.2 → NP_725203.1 imitation SWI, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_725203.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A4UZF0, B5X0J3, Q0E9A4, Q24368, Q8SX14, Q9V6E8

UniProtKB/TrEMBL

I1V4Y8

Related

FBpp0086955

Conserved Domains (8) summary

smart00717
Location:797 → 838

SANT; SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains

cd00046
Location:148 → 287

DEXDc; DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region.

cd00079
Location:422 → 553

HELICc; Helicase superfamily c-terminal domain; associated with DEXDc-, DEAD-, and DEAH-box proteins, yeast initiation factor 4A, Ski2p, and Hepatitis C virus NS3 helicases; this domain is found in a wide variety of helicases and helicase related proteins; may ...

cd00167
Location:799 → 838

SANT; 'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains. Tandem copies of the domain bind telomeric DNA tandem repeatsas part of the capping complex. Binding is sequence dependent for repeats which contain the G/C rich motif [C2-3 A (CA)1-6]. The domain is ...

pfam00176
Location:131 → 411

SNF2_N; SNF2 family N-terminal domain

pfam09110
Location:697 → 796

HAND; HAND

pfam09111
Location:853 → 965

SLIDE; SLIDE

pfam13892
Location:30 → 67

DBINO; DNA-binding domain