DCTN1-p150 Dynactin 1, p150 subunit [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: DCTN1-p150provided by FlyBase
Official Full Name: Dynactin 1, p150 subunitprovided by FlyBase
Primary source: FLYBASE:FBgn0001108
Locus tag: Dmel_CG9206
See related: AllianceGenome:FB:FBgn0001108
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG9206; DCTN; Dctn 1-p150; DCTN-P; Dctn1; DCTN1; Dmel\CG9206; DYNA_DROME; FCP-D; gl; Gl; Glu; Glued; glued/p150; p150; P150; p150/glued; p150/Glued; P150A; p150[glued]; p150[Glued]; P150[Glued]; t1
Summary: Enables dynein complex binding activity; dynein intermediate chain binding activity; and microtubule binding activity. Involved in several processes, including axonal transport; compound eye morphogenesis; and microtubule cytoskeleton organization. Located in several cellular components, including axon; microtubule cytoskeleton; and posterior cell cortex. Colocalizes with kinetochore; kinetochore microtubule; and spindle pole centrosome. Is expressed in several structures, including adult head; adult heart; germarium; germline cell; and gonad. Used to study Perry syndrome; amyotrophic lateral sclerosis; and motor neuron disease. Human ortholog(s) of this gene implicated in Perry syndrome; amyotrophic lateral sclerosis type 1; and autosomal dominant distal hereditary motor neuronopathy 14. Orthologous to human DCTN1 (dynactin subunit 1). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 70C5-70C6; 3-41 cM

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3L	NT_037436.4 (13929387..13934656)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3L	NT_037436.3 (13922487..13927756)

Chromosome 3L - NT_037436.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Dysregulation of stress granule dynamics by DCTN1 deficiency exacerbates TDP-43 pathology in Drosophila models of ALS/FTD.
Title: Dysregulation of stress granule dynamics by DCTN1 deficiency exacerbates TDP-43 pathology in Drosophila models of ALS/FTD.
the RNA is required for robust activation of dynein motility. We show that a cis-acting RNA localisation signal promotes the interaction of Egl with BicD, which licenses the latter protein to recruit dynein and dynactin. Our data support a model for BicD activation based on RNA-induced occupancy of two Egl-binding sites on the BicD dimer.
Title: RNA-directed activation of cytoplasmic dynein-1 in reconstituted transport RNPs.
artificially dimerized Egl activates dynein-dynactin-BicD in the absence of mRNA. The ability of mRNA cargo to orchestrate the activation of the mRNP (messenger ribonucleotide protein) complex is an elegant way to ensure that only cargo-bound motors are motile.
Title: Recruitment of two dyneins to an mRNA-dependent Bicaudal D transport complex.
Arfaptin physically associates with Glued and other dynactin complex components in the nervous system of both flies and mice and colocalizes with Glued at the Golgi in motor neurons.
Title: Normal dynactin complex function during synapse growth in Drosophila requires membrane binding by Arfaptin.
It uncovered an important requirement for Lis1 in promoting the recruitment of dynein and its accessory complex dynactin to RNA localization complexes.
Title: Lissencephaly-1 promotes the recruitment of dynein and dynactin to transported mRNAs.
show that this cell polarity regulator interacts with Glued during central synapse formation.
Title: A modifier screen in the Drosophila eye reveals that aPKC interacts with Glued during central synapse formation.
Dynein-dynactin plays a role in excluding dendritic Dscam from axons by retrograde transport.
Title: Dynein-dynactin complex is essential for dendritic restriction of TM1-containing Drosophila Dscam.
Mutants in the Lis1/dynactin complex strongly decrease maximum and average spindle velocity, consistent with this motor complex mediating spindle/cortex forces.
Title: Lis1/dynactin regulates metaphase spindle orientation in Drosophila neuroblasts.
multiple independent disruptions of Dynactin function cause a relocation of the photoreceptor nucleus toward the brain
Title: Dynactin is required to maintain nuclear position within postmitotic Drosophila photoreceptor neurons.
We conclude that Lis1/dynactin act together to regulate multiple, independent functions in mitotic cells, including spindle formation and cell cycle checkpoint release.
Title: Live imaging of Drosophila brain neuroblasts reveals a role for Lis1/dynactin in spindle assembly and mitotic checkpoint control.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables dynein complex binding	IDA Inferred from Direct Assay more info	PubMed
enables dynein complex binding	IPI Inferred from Physical Interaction more info	PubMed
enables dynein intermediate chain binding	IPI Inferred from Physical Interaction more info	PubMed
enables microtubule binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in axon extension	IGI Inferred from Genetic Interaction more info	PubMed
involved_in axonal transport of mitochondrion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell fate determination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellularization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in centrosome localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in centrosome separation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in compound eye photoreceptor cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in compound eye photoreceptor development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of epithelial cell apical/basal polarity	IDA Inferred from Direct Assay more info	PubMed
involved_in establishment of mitotic spindle orientation	IBA Inferred from Biological aspect of Ancestor more info
involved_in evoked neurotransmitter secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in eye photoreceptor cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular protein transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in kinetochore organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lumen formation, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in melanotic encapsulation of foreign target	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic cell cycle	HMP more info	PubMed
involved_in mitotic spindle midzone assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear migration	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of axonogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to kinetochore	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retrograde axonal transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retrograde neuronal dense core vesicle transport	IGI Inferred from Genetic Interaction more info	PubMed
involved_in retrograde neuronal dense core vesicle transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retrograde synaptic vesicle transport	IDA Inferred from Direct Assay more info	PubMed
involved_in rhabdomere development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in second mitotic wave involved in compound eye morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spindle assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in synaptic vesicle transport	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in aster	IDA Inferred from Direct Assay more info	PubMed
is_active_in axon	IBA Inferred from Biological aspect of Ancestor more info
located_in axon cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
part_of dynactin complex	ISS Inferred from Sequence or Structural Similarity more info
colocalizes_with kinetochore	IDA Inferred from Direct Assay more info	PubMed
located_in kinetochore	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with kinetochore microtubule	IDA Inferred from Direct Assay more info	PubMed
part_of microtubule associated complex	IBA Inferred from Biological aspect of Ancestor more info
located_in midbody	IDA Inferred from Direct Assay more info	PubMed
located_in posterior cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in spindle microtubule	IDA Inferred from Direct Assay more info	PubMed
is_active_in spindle pole	IBA Inferred from Biological aspect of Ancestor more info
colocalizes_with spindle pole centrosome	IDA Inferred from Direct Assay more info	PubMed
located_in type Ib terminal bouton	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: dynactin 1, p150 subunit

Names: CG9206-PA; DCTN1-p150-PA; Dynactin1 p150 A; dynactin; dynactin P150; glued; p150-Glued; p150[glued]; p150glued

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_037436.4 Reference assembly

Range

13929387..13934656

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_079337.3 → NP_524061.1 dynactin 1, p150 subunit [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_524061.1

Status: REVIEWED

UniProtKB/Swiss-Prot

B5X0J0, P13496, Q8MT52, Q9VUA1

Related

FBpp0075498

Conserved Domains (2) summary

smart01052
Location:8 → 74

CAP_GLY; Cytoskeleton-associated proteins (CAPs) are involved in the organisation of microtubules and transportation of vesicles and organelles along the cytoskeletal network

pfam12455
Location:553 → 834

Dynactin; Dynein associated protein