Ap2a2 adaptor related protein complex 2 subunit alpha 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Ap2a2provided by RGD
Official Full Name: adaptor related protein complex 2 subunit alpha 2provided by RGD
Primary source: RGD:71015
See related: EnsemblRapid:ENSRNOG00000019534 AllianceGenome:RGD:71015
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables phosphatidylinositol binding activity and protein serine/threonine kinase binding activity. Predicted to be involved in clathrin-dependent endocytosis and synaptic vesicle endocytosis. Part of AP-2 adaptor complex. Is active in synaptic vesicle. Orthologous to human AP2A2 (adaptor related protein complex 2 subunit alpha 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Kidney (RPKM 513.3), Brain (RPKM 486.7) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 1q41

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (206081856..206155146)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (196652315..196725609)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (214534217..214607497)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Evidence on differential role for alpha 1 and alpha 2 subtypes of AP-2 adaptin in the central nervous system.
Title: Evidence on differential role for alpha 1 and alpha 2 subtypes of AP-2 adaptin in the central nervous system.
AP2 binding to three ariginine residues motif in the GABA(A)R beta3-subunit regulates GABA(A)R residency time at endocytic zones, steady-state synaptic receptor levels, and pathological loss of GABA(A)Rs from synapses during simulated ischemia.
Title: Stabilization of GABA(A) receptors at endocytic zones is mediated by an AP2 binding motif within the GABA(A) receptor β3 subunit.
alpha-AP-2 has a role in directing myosin VI-dependent endocytosis of CFTR; myosin VI is involved in membrane invagination and coated pit formation in enterocytes
Title: Alpha-AP-2 directs myosin VI-dependent endocytosis of cystic fibrosis transmembrane conductance regulator chloride channels in the intestine.
AP-2 binds to unique sites on the C-terminus of NMDA receptor subunit NR2B and has an antagonistic functional consequence that is independent of the ability of PSD-95 to cluster receptors on the plasma membrane.
Title: Differential binding of the AP-2 adaptor complex and PSD-95 to the C-terminus of the NMDA receptor subunit NR2B regulates surface expression.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

X14972 (e-PCR)
- UniSTS:125800

RH128084 (e-PCR)
- UniSTS:211392

AA800186 (e-PCR)
- UniSTS:250598

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables clathrin adaptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables clathrin adaptor activity	IEA Inferred from Electronic Annotation more info
enables disordered domain specific binding	IEA Inferred from Electronic Annotation more info
enables disordered domain specific binding	ISO Inferred from Sequence Orthology more info
enables kinase binding	ISO Inferred from Sequence Orthology more info
enables phosphatidylinositol binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	ISO Inferred from Sequence Orthology more info
enables protein kinase binding	IEA Inferred from Electronic Annotation more info
enables protein kinase binding	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in biological_process	ND No biological Data available more info
involved_in clathrin-dependent endocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in clathrin-dependent endocytosis	IEA Inferred from Electronic Annotation more info
involved_in clathrin-dependent endocytosis	ISO Inferred from Sequence Orthology more info
involved_in clathrin-dependent endocytosis	ISS Inferred from Sequence or Structural Similarity more info
involved_in intracellular protein transport	IEA Inferred from Electronic Annotation more info
involved_in synaptic vesicle endocytosis	IEA Inferred from Electronic Annotation more info
involved_in synaptic vesicle endocytosis	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of AP-2 adaptor complex	IBA Inferred from Biological aspect of Ancestor more info
part_of AP-2 adaptor complex	IEA Inferred from Electronic Annotation more info
part_of AP-2 adaptor complex	IMP Inferred from Mutant Phenotype more info	PubMed
part_of AP-2 adaptor complex	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic vesicle	ISO Inferred from Sequence Orthology more info
part_of membrane coat	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	TAS Traceable Author Statement more info
located_in presynapse	IEA Inferred from Electronic Annotation more info
is_active_in synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in synapse	NAS Non-traceable Author Statement more info	PubMed
is_active_in synaptic vesicle	EXP Inferred from Experiment more info	PubMed
is_active_in synaptic vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in synaptic vesicle	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: AP-2 complex subunit alpha-2

Names: 100 kDa coated vesicle protein C; adapter-related protein complex 2 alpha-2 subunit; adapter-related protein complex 2 subunit alpha-2; adaptor protein complex AP-2 subunit alpha-2; adaptor protein complex AP-2, alpha 2 subunit; adaptor-related protein complex 2, alpha 2 subunit; alpha-adaptin C; alpha2-adaptin; clathrin assembly protein complex 2 alpha-C large chain; plasma membrane adaptor HA2/AP2 adaptin alpha C subunit

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_031008.2 → NP_112270.2 AP-2 complex subunit alpha-2

See identical proteins and their annotated locations for NP_112270.2

Status: PROVISIONAL

Source sequence(s)

BC081786

UniProtKB/Swiss-Prot

P18484

UniProtKB/TrEMBL

Q66HM2

Conserved Domains (4) summary

smart00809
Location:717 → 820

Alpha_adaptinC2; Adaptin C-terminal domain

sd00044
Location:332 → 355

HEAT; HEAT repeat [structural motif]

pfam02296
Location:826 → 934

Alpha_adaptin_C; Alpha adaptin AP2, C-terminal domain

pfam01602
Location:29 → 591

Adaptin_N; Adaptin N terminal region

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086019.1 Reference GRCr8

Range

206081856..206155146

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_008760010.4 → XP_008758232.1 AP-2 complex subunit alpha-2 isoform X1

UniProtKB/Swiss-Prot

P18484

UniProtKB/TrEMBL

F7F1Y0

Related

ENSRNOP00000060992.2, ENSRNOT00000064254.4

Conserved Domains (4) summary

smart00809
Location:716 → 819

Alpha_adaptinC2; Adaptin C-terminal domain

sd00044
Location:331 → 354

HEAT; HEAT repeat [structural motif]

pfam02296
Location:825 → 933

Alpha_adaptin_C; Alpha adaptin AP2, C-terminal domain

pfam01602
Location:29 → 590

Adaptin_N; Adaptin N terminal region