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    hypC hydrogenase maturation factor HypC [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947192, updated on 17-Dec-2024

    Summary

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    Official Symbol
    hypC
    Official Full Name
    hydrogenase maturation factor HypC
    Primary source
    ECOCYC:EG10485
    Locus tag
    b2728
    See related
    ASAP:ABE-0008962
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2723
    Summary
    N-terminal Cys is required for function. hypC mutants have reduced Hyd-2 activity. [More information is available at EcoGene: EG10485]. The HypC protein is an accessory protein primarily involved in the maturation of the hydrogenase 3 isoenzyme. [More information is available at EcoCyc: EG10485].
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    Genomic context

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    Sequence:
    NC_000913.3 (2851864..2852136)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene regulator of the transcriptional regulator FhlA Neighboring gene hydrogenase 3 nickel incorporation protein HypA Neighboring gene hydrogenase isoenzymes nickel incorporation protein HypB Neighboring gene Fe-(CN)2CO cofactor assembly scaffold protein HypD Neighboring gene carbamoyl dehydratase HypE Neighboring gene DNA-binding transcriptional activator FhlA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Solution structure of Escherichia coli HypC. Wang L, et al. Biochem Biophys Res Commun, 2007 Sep 28. PMID 17669368
    2. Heterologous complementation studies in Escherichia coli with the Hyp accessory protein machinery from Chloroflexi provide insight into [NiFe]-hydrogenase large subunit recognition by the HypC protein family. Hartwig S, et al. Microbiology (Reading), 2015 Nov. PMID 26364315
    3. Dissection of the maturation reactions of the [NiFe] hydrogenase 3 from Escherichia coli taking place after nickel incorporation. Magalon A, et al. FEBS Lett, 2000 May 12. PMID 10812085
    4. Maturation of [NiFe]-hydrogenases in Escherichia coli: the HypC cycle. Blokesch M, et al. J Mol Biol, 2002 Nov 22. PMID 12441107
    5. The hyp operon gene products are required for the maturation of catalytically active hydrogenase isoenzymes in Escherichia coli. Jacobi A, et al. Arch Microbiol, 1992. PMID 1482271

    See all (25) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Together, these findings identify amino acid regions in HypC accessory proteins that specify interaction with the large subunits of hydrogenase and demonstrate functional compatibility of Hyp accessory protein machineries.
      Title: Heterologous complementation studies in Escherichia coli with the Hyp accessory protein machinery from Chloroflexi provide insight into [NiFe]-hydrogenase large subunit recognition by the HypC protein family.
    2. Sequence alignment and structural comparison reveal that the hydrophobic region on the surface of E. coli HypC, as well as the highly flexible C-terminal helixes, may involve in the interactions of E. coli HypC with other proteins.
      Title: Solution structure of Escherichia coli HypC.
    3. N-terminus verified by Edman degradation on mature peptide
      Title: Analysis of the HypC-hycE complex, a key intermediate in the assembly of the metal center of the Escherichia coli hydrogenase 3.
    4. dynamic complex between the hydrogenase maturation proteins HypD, HypC, HypE and HypF, which is the site of ligand biosynthesis and attachment to the iron atom of the NiFe site in hydrogenase
      Title: The complex between hydrogenase-maturation proteins HypC and HypD is an intermediate in the supply of cyanide to the active site iron of [NiFe]-hydrogenases.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables carbon dioxide binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables carbon dioxide binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables iron ion binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables iron ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in protein maturation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein maturation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein maturation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein-containing complex assembly NAS
    Non-traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    hydrogenase maturation factor HypC

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2851864..2852136
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417208.1 hydrogenase maturation factor HypC [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417208.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A1X3I1J2, A0A1X3JDQ1, A0A8S0FMP1, A0AAJ3NX78, F4T456
      Conserved Domains (1) summary
      PRK10409
      Location:1 → 90
      PRK10409; HypC/HybG/HupF family hydrogenase formation chaperone
    Nucleotide Protein
    Heading Accession and Version

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