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    rssB regulator of RpoS [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945855, updated on 17-Dec-2024

    Summary

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    Official Symbol
    rssB
    Official Full Name
    regulator of RpoS
    Primary source
    ECOCYC:EG12121
    Locus tag
    b1235
    See related
    ASAP:ABE-0004147
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1230; hnr; sprE; ychL
    Summary
    A yliE rssB9(hnr) double null mutant has significantly increased lycopene production in 1X M9; the effect may be salt-suppressible as it is not seen in 2X M9 (Alper, 2008). [More information is available at EcoGene: EG12121]. RssB facilitates and regulates degradation of sigma S by the protease ClpXP during normal nutrient conditions . [More information is available at EcoCyc: EG12121].
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    Genomic context

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    Sequence:
    NC_000913.3 (1290242..1291255)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene NTF2-like domain-containing protein YchJ Neighboring gene putative patatin-like phospholipase RssA Neighboring gene UTP--glucose-1-phosphate uridylyltransferase Neighboring gene DNA-binding transcriptional dual regulator H-NS

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Phospho-dependent signaling during the general stress response by the atypical response regulator and ClpXP adaptor RssB. Schwartz J, et al. Protein Sci, 2021 Apr. PMID 33599047, Free PMC Article
    2. Insight into the RssB-Mediated Recognition and Delivery of σ(s) to the AAA+ Protease, ClpXP. Micevski D, et al. Biomolecules, 2020 Apr 16. PMID 32316259, Free PMC Article
    3. The response regulator SprE controls the stability of RpoS. Pratt LA, et al. Proc Natl Acad Sci U S A, 1996 Mar 19. PMID 8637901, Free PMC Article
    4. Regulation of proteolysis of the stationary-phase sigma factor RpoS. Zhou Y, et al. J Bacteriol, 1998 Mar. PMID 9495753, Free PMC Article
    5. SprE levels are growth phase regulated in a sigma(S)-dependent manner at the level of translation. Gibson KE, et al. J Bacteriol, 2000 Jul. PMID 10869095, Free PMC Article

    See all (54) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Structure of phosphorylated-like RssB, the adaptor delivering sigma[s] to the ClpXP proteolytic machinery, reveals an interface switch for activation.
      Title: Structure of phosphorylated-like RssB, the adaptor delivering σ(s) to the ClpXP proteolytic machinery, reveals an interface switch for activation.
    2. Phospho-dependent signaling during the general stress response by the atypical response regulator and ClpXP adaptor RssB.
      Title: Phospho-dependent signaling during the general stress response by the atypical response regulator and ClpXP adaptor RssB.
    3. crystal structure of RssB bound to an antiadaptor, the DNA damage-inducible IraD; demonstrate that (1) in the presence of IraD, RssB adopts predominantly a closed conformation; (2) IraD contacts both domains of RssB; (3) the interactions observed in the structure are crucial for blocking sigmaS degradation in vivo and in vitro
      Title: Structural basis for inhibition of a response regulator of σ(S) stability by a ClpXP antiadaptor.
    4. Data propose that SprE plays a multifunctional role in controlling the transcriptome, regulating what is made via its effects on RpoS, and modulating what is degraded via its effects on polyadenylation and turnover of specific mRNAs.
      Title: The response regulator SprE (RssB) modulates polyadenylation and mRNA stability in Escherichia coli.
    5. IraD promotes accumulation of RpoS by binding to the adaptor RssB protein that targets RpoS for degradation.
      Title: A DNA damage response in Escherichia coli involving the alternative sigma factor, RpoS.
    6. IraM (inhibitor of RssB activity during Magnesium starvation) and IraD (inhibitor of RssB activity after DNA damage) inhibit sigmaS proteolysis both in vivo and in vitro.
      Title: Multiple pathways for regulation of sigmaS (RpoS) stability in Escherichia coli via the action of multiple anti-adaptors.
    7. The rssB mutant displays enhanced resistance to multiple external stresses, possibly indicating that the cell gains its increased vitality through elevated resistance to spontaneous, endogenous stresses.
      Title: Mutations in two global regulators lower individual mortality in Escherichia coli.
    8. Our data support the hypothesis that SprE activity is the key level at which ammonia and glucose starvation signals are transmitted to RpoS
      Title: Starvation for different nutrients in Escherichia coli results in differential modulation of RpoS levels and stability.
    9. phosphorylation site not required for regulation of RpoS proteolysis.
      Title: RpoS proteolysis is regulated by a mechanism that does not require the SprE (RssB) response regulator phosphorylation site.
    10. novel mechanism of regulation of sigma(S) stability through the regulation of RssB activity
      Title: Modulating RssB activity: IraP, a novel regulator of sigma(S) stability in Escherichia coli.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables phosphorelay response regulator activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables sigma factor antagonist activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables transcription cis-regulatory region binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Process Evidence Code Pubs
    involved_in negative regulation of DNA-templated transcription NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in phosphorelay signal transduction system IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within positive regulation of proteolysis EXP
    Inferred from Experiment
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of proteolysis IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of proteolysis IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within protein destabilization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of DNA-templated transcription IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in regulation of gene expression IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of protein-DNA complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of sigma factor antagonist complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    regulator of RpoS

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1290242..1291255
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415751.1 regulator of RpoS [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415751.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A2H4TM78
      Conserved Domains (2) summary
      PRK10693
      Location:36 → 337
      PRK10693; two-component system response regulator RssB
      cl19078
      Location:10 → 59
      REC; phosphoacceptor receiver (REC) domain of response regulators (RRs) and pseudo response regulators (PRRs)
    Nucleotide Protein
    Heading Accession and Version

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