BFN1 bifunctional nuclease i [Arabidopsis thaliana (thale cress)] - Gene

Summary

Official Symbol: BFN1
Official Full Name: bifunctional nuclease i
Primary source: TAIR:AT1G11190
Locus tag: AT1G11190
See related: Araport:AT1G11190
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: bifunctional nuclease i; ENDO1; ENDONUCLEASE 1; T28P6.14; T28P6_14
Summary: Encodes a bifunctional nuclease that acts on both RNA and DNA involved in nucleic acid degradation to facilitate nucleotide and phosphate recovery during senescence. It has mismatch-specific endonuclease activity with wide recognition of single base mismatches as well as the ability to cleave indel types of mismatches (heteroduplexes with loops).
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Chromosome 1 - NC_003070.9 Genomic Context describing neighboring genes

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Localization of the Arabidopsis senescence- and cell death-associated BFN1 nuclease: from the ER to fragmented nuclei.
Title: Localization of the Arabidopsis senescence- and cell death-associated BFN1 nuclease: from the ER to fragmented nuclei.
BIFUNCTIONAL NUCLEASE1 (BFN1), a well-known senescence-enhanced gene, is directly regulated by ORE1.
Title: NAC transcription factor ORE1 and senescence-induced BIFUNCTIONAL NUCLEASE1 (BFN1) constitute a regulatory cascade in Arabidopsis.
Data indicate that five S1-like nucleases have been named as ENDO1 (BFN1, AT1G11190), ENDO2 (AT1G68290), ENDO3 (AT4G21590), ENDO4 (AT4G21585) and ENDO5 (AT4G21600).
Title: The plant s1-like nuclease family has evolved a highly diverse range of catalytic capabilities.
BFN1 (ENDO1) is a Ca2+/Mn2+-dependent nuclease, acting at neutral pH.
Title: The plant s1-like nuclease family has evolved a highly diverse range of catalytic capabilities.
BFN1 is involved in programmed cell death associated with different development processes in Arabidopsis.
Title: Expression analysis of the BFN1 nuclease gene promoter during senescence, abscission, and programmed cell death-related processes.
ENDO1 detects and cleaves double-stranded DNA at point mutations with high specificity.
Title: Characterization of Arabidopsis thaliana mismatch specific endonucleases: application to mutation discovery by TILLING in pea.

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NM_100991.2

Function	Evidence Code	Pubs
enables RNA endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA endonuclease activity, producing 5'-phosphomonoesters	IDA Inferred from Direct Assay more info	PubMed
enables T/G mismatch-specific endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables double-stranded DNA endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables single-stranded DNA endodeoxyribonuclease activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in floral organ senescence	IEP Inferred from Expression Pattern more info	PubMed
involved_in leaf senescence	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
located_in extracellular region	ISM Inferred from Sequence Model more info

NP_172585.1

bifunctional nuclease i (BFN1); FUNCTIONS IN: T/G mismatch-specific endonuclease activity, endoribonuclease activity, producing 5'-phosphomonoesters, single-stranded DNA specific endodeoxyribonuclease activity, nucleic acid binding; INVOLVED IN: DNA catabolic process; LOCATED IN: endomembrane system; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: 8 growth stages; CONTAINS InterPro DOMAIN/s: Phospholipase C/P1 nuclease, core (InterPro:IPR008947), S1/P1 nuclease (InterPro:IPR003154); BEST Arabidopsis thaliana protein match is: endonuclease 4 (TAIR:AT4G21585.1); Has 701 Blast hits to 698 proteins in 200 species: Archae - 0; Bacteria - 187; Metazoa - 0; Fungi - 98; Plants - 121; Viruses - 4; Other Eukaryotes - 291 (source: NCBI BLink).