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    WRI1 Integrase-type DNA-binding superfamily protein [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 824599, updated on 18-Sep-2024

    Summary

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    Official Symbol
    WRI1
    Official Full Name
    Integrase-type DNA-binding superfamily protein
    Primary source
    TAIR:AT3G54320
    Locus tag
    AT3G54320
    See related
    Araport:AT3G54320
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    ACTIVATOR OF SPO(MIN)::LUC1; ASML1; ATWRI1; WRI; WRINKLED; WRINKLED 1
    Summary
    WRINKLED1 encodes transcription factor of the AP2/ERWEBP class. Protein has two plant-specific (AP2/EREB) DNA-binding domains and is involved in the control of storage compound biosynthesis in Arabidopsis. Mutants have wrinkled seed phenotype, due to a defect in the incorporation of sucrose and glucose into triacylglycerols. Transgenic sGsL plants (21-day-old) grown on 6% sucrose for 24 hours had 2-fold increase in levels of expressions (sGsL line carries a single copy of T-DNA containing the Spomin::GUS-Spomin::LUC dual reporter genes in the upper arm of chromosome 5 of ecotype Col-0. The sporamin .minimal. promoter directs sugar-inducible expression of the LUC and GUS reporters in leaves). Regulation by LEC2 promotes fatty acid accumulation during seed maturation.
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    Genomic context

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    See WRI1 in Genome Data Viewer
    Location:
    chromosome: 3
    Exon count:
    6
    Sequence:
    Chromosome: 3; NC_003074.8 (20114488..20118745)

    Chromosome 3 - NC_003074.8Genomic Context describing neighboring genes Neighboring gene vesicle-associated membrane protein 727 Neighboring gene DNA-directed RNA polymerase subunit alpha Neighboring gene K-box region and MADS-box transcription factor family protein Neighboring gene Forkhead-associated (FHA) domain-containing protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003074.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. A WRI1-dependent module is essential for the accumulation of auxin and lipid in somatic embryogenesis of Arabidopsis thaliana. Zhang WJ, et al. New Phytol, 2024 May. PMID 38515249
    2. Wrinkled1, a ubiquitous regulator in oil accumulating tissues from Arabidopsis embryos to oil palm mesocarp. Ma W, et al. PLoS One, 2013. PMID 23922666, Free PMC Article
    3. Role of WRINKLED1 in the transcriptional regulation of glycolytic and fatty acid biosynthetic genes in Arabidopsis. Baud S, et al. Plant J, 2009 Dec. PMID 19719479
    4. WRINKLED1 Rescues Feedback Inhibition of Fatty Acid Synthesis in Hydroxylase-Expressing Seeds. Adhikari ND, et al. Plant Physiol, 2016 May. PMID 27208047, Free PMC Article
    5. An AP2-type transcription factor, WRINKLED1, of Arabidopsis thaliana binds to the AW-box sequence conserved among proximal upstream regions of genes involved in fatty acid synthesis. Maeo K, et al. Plant J, 2009 Nov. PMID 19594710

    See all (31) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. A WRI1-dependent module is essential for the accumulation of auxin and lipid in somatic embryogenesis of Arabidopsis thaliana.
      Title: A WRI1-dependent module is essential for the accumulation of auxin and lipid in somatic embryogenesis of Arabidopsis thaliana.
    2. Transcription factor bZIP52 modulates Arabidopsis seed oil biosynthesis through interaction with WRINKLED1.
      Title: Transcription factor bZIP52 modulates Arabidopsis seed oil biosynthesis through interaction with WRINKLED1.
    3. The function of the WRI1-TCP4 regulatory module in lipid biosynthesis.
      Title: The function of the WRI1-TCP4 regulatory module in lipid biosynthesis.
    4. TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR4 Interacts with WRINKLED1 to Mediate Seed Oil Biosynthesis.
      Title: TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR4 Interacts with WRINKLED1 to Mediate Seed Oil Biosynthesis.
    5. WRINKLED1 Regulates BIOTIN ATTACHMENT DOMAIN-CONTAINING Proteins that Inhibit Fatty Acid Synthesis.
      Title: WRINKLED1 Regulates BIOTIN ATTACHMENT DOMAIN-CONTAINING Proteins that Inhibit Fatty Acid Synthesis.
    6. Mature seeds from TCDD-treated plants had a characteristic 'wrinkled' phenotype, due to a two-thirds reduction in storage oil content. There were increases in WRI1, which is puzzling in view of the low seed oil phenotype. This anomaly was explained by increased expression of 20S proteasome components that resulted in a substantial degradation of WRI1 protein.
      Title: Arabidopsis plants exposed to dioxin result in a WRINKLED seed phenotype due to 20S proteasomal degradation of WRI1.
    7. WRI1 phosphorylation and degradation is part of trehalose-6-phosphate pathway.
      Title: Trehalose 6-Phosphate Positively Regulates Fatty Acid Synthesis by Stabilizing WRINKLED1.
    8. This study identifies the first two regulatory genes, WRI1 and DGAT1, that control the synthesis of all tocochromanol forms in seeds, and shows the existence of a metabolic trade-off between lipid and tocochromanol metabolisms.
      Title: WRINKLED1 and ACYL-COA:DIACYLGLYCEROL ACYLTRANSFERASE1 regulate tocochromanol metabolism in Arabidopsis.
    9. MED15 is a target of WRI1.
      Title: The Mediator Complex MED15 Subunit Mediates Activation of Downstream Lipid-Related Genes by the WRINKLED1 Transcription Factor.
    10. WRINKLED1 transcription factor role in the auxin homeostasis in roots
      Title: The Arabidopsis WRINKLED1 transcription factor affects auxin homeostasis in roots.
    Submit: New GeneRIF Correction See all GeneRIFs (22)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    NM_001035780.3
    NM_001339658.1
    NM_001339660.1
    NM_115292.4

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA-binding transcription factor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-binding transcription factor activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    enables kinase binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within lipid biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within lipid metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of cutin biosynthetic process IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within regulation of carbohydrate metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within regulation of glycolytic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to sucrose IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within triglyceride biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in nucleus IC
    Inferred by Curator
    more info
    		 PubMed 
    located_in nucleus ISM
    Inferred from Sequence Model
    more info
    		  

    General protein information

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    Preferred Names
    Integrase-type DNA-binding superfamily protein
    NP_001030857.1
    • WRINKLED 1 (WRI1); CONTAINS InterPro DOMAIN/s: DNA-binding, integrase-type (InterPro:IPR016177), Pathogenesis-related transcriptional factor/ERF, DNA-binding (InterPro:IPR001471); BEST Arabidopsis thaliana protein match is: Integrase-type DNA-binding superfamily protein (TAIR:AT1G79700.2); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
    NP_001319744.1
    • WRINKLED 1 (WRI1); CONTAINS InterPro DOMAIN/s: DNA-binding, integrase-type (InterPro:IPR016177), Pathogenesis-related transcriptional factor/ERF, DNA-binding (InterPro:IPR001471); BEST Arabidopsis thaliana protein match is: Integrase-type DNA-binding superfamily protein (TAIR:AT4G37750.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
    NP_191000.3
    • WRINKLED 1 (WRI1); CONTAINS InterPro DOMAIN/s: DNA-binding, integrase-type (InterPro:IPR016177), Pathogenesis-related transcriptional factor/ERF, DNA-binding (InterPro:IPR001471); BEST Arabidopsis thaliana protein match is: Integrase-type DNA-binding superfamily protein (TAIR:AT1G79700.2); Has 9494 Blast hits to 5549 proteins in 332 species: Archae - 18; Bacteria - 68; Metazoa - 1307; Fungi - 140; Plants - 7359; Viruses - 82; Other Eukaryotes - 520 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003074.8 Reference assembly

      Range
      20114488..20118745
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001339659.1 → NP_001325502.1  Integrase-type DNA-binding superfamily protein [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A178V8G7, A0A1I9LLF6

    2. NM_001035780.3 → NP_001030857.1  Integrase-type DNA-binding superfamily protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_001030857.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q2V3P6, Q6J9N7, Q6X5Y6, Q9M2V3, Q9M2V4
      UniProtKB/TrEMBL
      A0A5S9XKQ4, A0A654FFP9
      Conserved Domains (2) summary
      smart00380
      Location:65 → 137
      AP2; DNA-binding domain in plant proteins such as APETALA2 and EREBPs
      cd00018
      Location:166 → 227
      AP2; DNA-binding domain found in transcription regulators in plants such as APETALA2 and EREBP (ethylene responsive element binding protein). In EREBPs the domain specifically binds to the 11bp GCC box of the ethylene response element (ERE), a promotor ...

    3. NM_115292.4 → NP_191000.3  Integrase-type DNA-binding superfamily protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_191000.3

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A654FFP9
      Conserved Domains (2) summary
      smart00380
      Location:65 → 137
      AP2; DNA-binding domain in plant proteins such as APETALA2 and EREBPs
      cd00018
      Location:166 → 227
      AP2; DNA-binding domain found in transcription regulators in plants such as APETALA2 and EREBP (ethylene responsive element binding protein). In EREBPs the domain specifically binds to the 11bp GCC box of the ethylene response element (ERE), a promotor ...

    4. NM_001339660.1 → NP_001325503.1  Integrase-type DNA-binding superfamily protein [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A178V8G7, A0A1I9LLF6

    5. NM_001339658.1 → NP_001319744.1  Integrase-type DNA-binding superfamily protein [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A8MS57
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q6X5Y6.1

    Gene LinkOut

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