Nos2 nitric oxide synthase 2, inducible [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Nos2provided by MGI
Official Full Name: nitric oxide synthase 2, inducibleprovided by MGI
Primary source: MGI:MGI:97361
See related: Ensembl:ENSMUSG00000020826 AllianceGenome:MGI:97361
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: iNOS; Nos-2; Nos2a; i-NOS; NOS-II; MAC-NOS
Summary: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase that is inducible by a combination of lipopolysaccharide and certain cytokines. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
Expression: Biased expression in large intestine adult (RPKM 30.6), ovary adult (RPKM 13.0) and 6 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 B5; 11 46.74 cM

Exon count:: 29

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (78811613..78851052)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (78920787..78960226)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

NOS2-derived low levels of NO drive psoriasis pathogenesis.
Title: NOS2-derived low levels of NO drive psoriasis pathogenesis.
iNOS regulates hematopoietic stem and progenitor cells via mitochondrial signaling and is critical for bone marrow regeneration.
Title: iNOS regulates hematopoietic stem and progenitor cells via mitochondrial signaling and is critical for bone marrow regeneration.
INOS ablation promotes corneal wound healing via activation of Akt signaling.
Title: INOS ablation promotes corneal wound healing via activation of Akt signaling.
iNOS is necessary for GBP-mediated T. gondii clearance in murine macrophages via vacuole nitration and intravacuolar network collapse.
Title: iNOS is necessary for GBP-mediated T. gondii clearance in murine macrophages via vacuole nitration and intravacuolar network collapse.
Potential i-Nos/Arg-1 Switch with NLRP3 and Parasitic Load Down Regulation in Experimental Schistosoma mansoni Infection via Chloroquine Repurposing.
Title: Potential i-Nos/Arg-1 Switch with NLRP3 and Parasitic Load Down Regulation in Experimental Schistosoma mansoni Infection via Chloroquine Repurposing.
Versican accumulation drives Nos2 induction and aortic disease in Marfan syndrome via Akt activation.
Title: Versican accumulation drives Nos2 induction and aortic disease in Marfan syndrome via Akt activation.
Inducible nitric oxide synthase accelerates nonalcoholic fatty liver disease progression by regulating macrophage autophagy.
Title: Inducible nitric oxide synthase accelerates nonalcoholic fatty liver disease progression by regulating macrophage autophagy.
Paneth cell-derived iNOS is required to maintain homeostasis in the intestinal stem cell niche.
Title: Paneth cell-derived iNOS is required to maintain homeostasis in the intestinal stem cell niche.
An innate granuloma eradicates an environmental pathogen using Gsdmd and Nos2.
Title: An innate granuloma eradicates an environmental pathogen using Gsdmd and Nos2.
Melatonin mediated inhibition of EZH2-NOS2 crosstalk attenuates inflammatory bowel disease in preclinical in vitro and in vivo models.
Title: Melatonin mediated inhibition of EZH2-NOS2 crosstalk attenuates inflammatory bowel disease in preclinical in vitro and in vivo models.

Submit: New GeneRIF Correction See all GeneRIFs (707)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (6) 1 citation
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

PMC201050P1 (e-PCR)
- UniSTS:271879

D11Bhm160 (e-PCR), detects polymorphism
- UniSTS:141598

D11Bhm161 (e-PCR), detects polymorphism
- UniSTS:141599

D11Bhm162 (e-PCR), detects polymorphism
- UniSTS:141600

D11Bhm163 (e-PCR), detects polymorphism
- UniSTS:141601

M84373 (e-PCR)
- UniSTS:124991

GDB:437694 (e-PCR)
- UniSTS:157251

PMC122623P2 (e-PCR)
- UniSTS:270406

PMC186377P3 (e-PCR)
- UniSTS:271704

D10Mco42 (e-PCR)
- UniSTS:240221

D11Mit144 (e-PCR), detects polymorphism
- UniSTS:126337

Nos2 (e-PCR), detects polymorphism
- UniSTS:525459

Nos2 (e-PCR), detects polymorphism
- UniSTS:532120

Nos2 (e-PCR), detects polymorphism
- UniSTS:144039

Nos2 (e-PCR), detects polymorphism
- UniSTS:144040

Nos2 (e-PCR), detects polymorphism
- UniSTS:144041

Nos2 (e-PCR), detects polymorphism
- UniSTS:144042

Nos2 (e-PCR), detects polymorphism
- UniSTS:499128

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables FMN binding	IBA Inferred from Biological aspect of Ancestor more info
enables FMN binding	IDA Inferred from Direct Assay more info	PubMed
enables Hsp90 protein binding	ISO Inferred from Sequence Orthology more info
enables NADP binding	IEA Inferred from Electronic Annotation more info
enables actin binding	ISO Inferred from Sequence Orthology more info
enables arginine binding	IDA Inferred from Direct Assay more info	PubMed
enables beta-catenin binding	ISO Inferred from Sequence Orthology more info
enables cAMP-dependent protein kinase regulator activity	ISO Inferred from Sequence Orthology more info
enables cadherin binding	ISO Inferred from Sequence Orthology more info
enables calmodulin binding	TAS Traceable Author Statement more info	PubMed
enables flavin adenine dinucleotide binding	IBA Inferred from Biological aspect of Ancestor more info
enables flavin adenine dinucleotide binding	IDA Inferred from Direct Assay more info	PubMed
enables heme binding	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nitric-oxide synthase activity	IBA Inferred from Biological aspect of Ancestor more info
enables nitric-oxide synthase activity	IDA Inferred from Direct Assay more info	PubMed
enables nitric-oxide synthase activity	ISO Inferred from Sequence Orthology more info
enables nitric-oxide synthase binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase binding	ISO Inferred from Sequence Orthology more info
enables tetrahydrobiopterin binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in Fc-gamma receptor signaling pathway involved in phagocytosis	IEA Inferred from Electronic Annotation more info
involved_in G protein-coupled receptor signaling pathway coupled to cGMP nucleotide second messenger	ISO Inferred from Sequence Orthology more info
involved_in arginine catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in arginine catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in arginine catabolic process	ISO Inferred from Sequence Orthology more info
involved_in blood vessel remodeling	ISO Inferred from Sequence Orthology more info
involved_in cGMP-mediated signaling	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to cytokine stimulus	ISO Inferred from Sequence Orthology more info	PubMed
acts_upstream_of_or_within cellular response to lipopolysaccharide	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cellular response to organic cyclic compound	ISO Inferred from Sequence Orthology more info	PubMed
acts_upstream_of_or_within cellular response to type II interferon	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cellular response to xenobiotic stimulus	IDA Inferred from Direct Assay more info	PubMed
involved_in circadian rhythm	IEP Inferred from Expression Pattern more info	PubMed
involved_in defense response to bacterium	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within defense response to bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in defense response to bacterium	ISO Inferred from Sequence Orthology more info
involved_in inflammatory response	IBA Inferred from Biological aspect of Ancestor more info
involved_in inflammatory response	TAS Traceable Author Statement more info	PubMed
involved_in intracellular signal transduction	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of blood pressure	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of gene expression	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in nitric oxide biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in nitric oxide biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in nitric oxide biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in nitric oxide mediated signal transduction	IBA Inferred from Biological aspect of Ancestor more info
NOT acts_upstream_of_or_within ovulation from ovarian follicle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in peptidyl-cysteine S-nitrosylation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of interleukin-6 production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of interleukin-6 production	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of interleukin-8 production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of interleukin-8 production	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of killing of cells of another organism	ISO Inferred from Sequence Orthology more info
involved_in prostaglandin secretion	ISO Inferred from Sequence Orthology more info
involved_in prostaglandin secretion	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of blood pressure	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of cell population proliferation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of cytokine production involved in inflammatory response	ISO Inferred from Sequence Orthology more info
involved_in regulation of cytokine production involved in inflammatory response	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of insulin secretion	IEA Inferred from Electronic Annotation more info
involved_in regulation of insulin secretion	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to bacterium	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to hormone	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within response to hypoxia	IDA Inferred from Direct Assay more info	PubMed
involved_in response to lipopolysaccharide	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to lipopolysaccharide	IDA Inferred from Direct Assay more info	PubMed
involved_in response to tumor necrosis factor	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within superoxide metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cortical cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	TAS Traceable Author Statement more info	PubMed
located_in extracellular space	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in peroxisome	IEA Inferred from Electronic Annotation more info
located_in peroxisome	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: nitric oxide synthase, inducible

Names: NOS type II; inducible NO synthase; inducible NOS; inducible nitric oxide synthase; macrophage NOS; nitric oxide synthase 2, inducible, macrophage; peptidyl-cysteine S-nitrosylase NOS2

NP_001300850.1

EC 1.14.13.39

NP_001300851.1

EC 1.14.13.39

NP_035057.1

EC 1.14.13.39

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001313921.1 → NP_001300850.1 nitric oxide synthase, inducible isoform b

Status: REVIEWED

Description

Transcript Variant: This variant (2) contains an alternate exon in place of the first two exons compared to variant 1. This difference results in translation initiation at a downstream start codon and an isoform (b) that is shorter at the N-terminus compared to isoform a. Variants 2 and 3 both encode the same isoform (b).

Source sequence(s)

AL592185, CJ057491, M87039

UniProtKB/TrEMBL

A0A1L1STZ4

Conserved Domains (4) summary

COG0369
Location:421 → 1008

CysJ; Sulfite reductase, alpha subunit (flavoprotein) [Inorganic ion transport and metabolism]

cd00795
Location:1 → 377

NOS_oxygenase_euk; Nitric oxide synthase (NOS) eukaryotic oxygenase domain. NOS produces nitric oxide (NO) by catalyzing a five-electron heme-based oxidation of a guanidine nitrogen of L-arginine to L-citrulline via two successive monooxygenation reactions producing N ...

pfam00258
Location:422 → 553

Flavodoxin_1; Flavodoxin

cl06868
Location:617 → 1013

FNR_like; Ferredoxin reductase (FNR), an FAD and NAD(P) binding protein, was intially identified as a chloroplast reductase activity, catalyzing the electron transfer from reduced iron-sulfur protein ferredoxin to NADP+ as the final step in the electron transport ...
NM_001313922.1 → NP_001300851.1 nitric oxide synthase, inducible isoform b

Status: REVIEWED

Description

Transcript Variant: This variant (3) contains an alternate exon in place of the first two exons compared to variant 1. This difference results in translation initiation at a downstream start codon and an isoform (b) that is shorter at the N-terminus compared to isoform a. Variants 2 and 3 both encode the same isoform (b).

Source sequence(s)

AL592185, CN693247, M87039

UniProtKB/TrEMBL

A0A1L1STZ4

Related

ENSMUSP00000150558.3, ENSMUST00000214397.3

Conserved Domains (4) summary

COG0369
Location:421 → 1008

CysJ; Sulfite reductase, alpha subunit (flavoprotein) [Inorganic ion transport and metabolism]

cd00795
Location:1 → 377

NOS_oxygenase_euk; Nitric oxide synthase (NOS) eukaryotic oxygenase domain. NOS produces nitric oxide (NO) by catalyzing a five-electron heme-based oxidation of a guanidine nitrogen of L-arginine to L-citrulline via two successive monooxygenation reactions producing N ...

pfam00258
Location:422 → 553

Flavodoxin_1; Flavodoxin

cl06868
Location:617 → 1013

FNR_like; Ferredoxin reductase (FNR), an FAD and NAD(P) binding protein, was intially identified as a chloroplast reductase activity, catalyzing the electron transfer from reduced iron-sulfur protein ferredoxin to NADP+ as the final step in the electron transport ...
NM_010927.4 → NP_035057.1 nitric oxide synthase, inducible isoform a

See identical proteins and their annotated locations for NP_035057.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a).

Source sequence(s)

AL592185, M87039

Consensus CDS

CCDS25115.1

UniProtKB/Swiss-Prot

O70515, O70516, P29477, Q5SXT3, Q6P6A0, Q8R410

UniProtKB/TrEMBL

A0A1L1STZ4

Related

ENSMUSP00000018610.7, ENSMUST00000018610.7

Conserved Domains (4) summary

COG0369
Location:534 → 1121

CysJ; Sulfite reductase, alpha subunit (flavoprotein) [Inorganic ion transport and metabolism]

cd00795
Location:78 → 490

NOS_oxygenase_euk; Nitric oxide synthase (NOS) eukaryotic oxygenase domain. NOS produces nitric oxide (NO) by catalyzing a five-electron heme-based oxidation of a guanidine nitrogen of L-arginine to L-citrulline via two successive monooxygenation reactions producing N ...

pfam00258
Location:535 → 666

Flavodoxin_1; Flavodoxin

cl06868
Location:730 → 1126

FNR_like; Ferredoxin reductase (FNR), an FAD and NAD(P) binding protein, was intially identified as a chloroplast reductase activity, catalyzing the electron transfer from reduced iron-sulfur protein ferredoxin to NADP+ as the final step in the electron transport ...