Gnai3 G protein subunit alpha i3 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Gnai3provided by RGD
Official Full Name: G protein subunit alpha i3provided by RGD
Primary source: RGD:2714
See related: AllianceGenome:RGD:2714
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: GIP3A
Summary: Enables G protein-coupled serotonin receptor binding activity; GTPase activating protein binding activity; and protein domain specific binding activity. Involved in several processes, including positive regulation of NAD(P)H oxidase activity; positive regulation of superoxide anion generation; and positive regulation of vascular associated smooth muscle cell proliferation. Located in plasma membrane and zymogen granule. Biomarker of hypertension. Orthologous to human GNAI3 (G protein subunit alpha i3). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Lung (RPKM 357.2), Kidney (RPKM 356.3) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 2q34

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	2	NC_086020.1 (198430920..198468874, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	2	NC_051337.1 (195742765..195780720, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	2	NC_005101.4 (210896779..210934733, complement)

Chromosome 2 - NC_086020.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Nitric oxide decreased the enhanced expression of Gialpha-2/3 proteins and hyperproliferation of Vascular smooth muscle cells from spontaneously hypertensive rats by cGMP-independent mechanism.
Title: Nitric oxide attenuates overexpression of Giα proteins in vascular smooth muscle cells from SHR: Role of ROS and ROS-mediated signaling.
These results indicate that NPR-C activation by C-ANP4-23 attenuates the enhanced levels of cell cycle proteins through the inhibition of enhanced expression of Gialpha proteins.
Title: Natriuretic peptide receptor-C agonist attenuates the expression of cell cycle proteins and proliferation of vascular smooth muscle cells from spontaneously hypertensive rats: role of Gi proteins and MAPkinase/PI3kinase signaling.
Results suggest that the suppression of Dopamine-2 (D2) receptor short (D2S)-regulated Gi3 inhibition of Gs protein may be one of the mechanisms controlling estradiol (E(2)-activated prolactin (PRL) synthesis and cell proliferation in lactotropes.
Title: Estrogen inhibits D2S receptor-regulated Gi3 and Gs protein interactions to stimulate prolactin production and cell proliferation in lactotropic cells.
Suggest role for oxidative stress in high glucose-induced decreased expression of Gnai2/3 and adenylyl cyclase signaling in vascular smooth muscle cells.
Title: Role of oxidative stress in high glucose-induced decreased expression of Gialpha proteins and adenylyl cyclase signaling in vascular smooth muscle cells.
studies suggest a possible role for zymogen granule-associated G(alpha)(i3) in zymogen granule-plasma membrane fusion
Title: G(alpha)(i3) in pancreatic zymogen granules participates in vesicular fusion.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH130562 (e-PCR)
- UniSTS:213846

BF417859 (e-PCR)
- UniSTS:249697

RH94416 (e-PCR)
- UniSTS:119664

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables G protein-coupled receptor binding	IBA Inferred from Biological aspect of Ancestor more info
enables G protein-coupled serotonin receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables G-protein beta/gamma-subunit complex binding	IBA Inferred from Biological aspect of Ancestor more info
enables G-protein beta/gamma-subunit complex binding	IEA Inferred from Electronic Annotation more info
enables GDP binding	IEA Inferred from Electronic Annotation more info
enables GDP binding	ISO Inferred from Sequence Orthology more info
enables GDP binding	ISS Inferred from Sequence or Structural Similarity more info
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activating protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	IEA Inferred from Electronic Annotation more info
enables GTPase activity	ISO Inferred from Sequence Orthology more info
enables GTPase activity	ISS Inferred from Sequence or Structural Similarity more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in G protein-coupled dopamine receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in GTP metabolic process	IEA Inferred from Electronic Annotation more info
involved_in GTP metabolic process	ISO Inferred from Sequence Orthology more info
involved_in GTP metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in adenylate cyclase-modulating G protein-coupled receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in cell division	ISO Inferred from Sequence Orthology more info
involved_in cell division	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of apoptotic signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of secretion	TAS Traceable Author Statement more info	PubMed
involved_in positive regulation of NAD(P)H oxidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of macroautophagy	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of macroautophagy	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of superoxide anion generation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of vascular associated smooth muscle cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
NOT acts_upstream_of_or_within regulation of heart contraction	ISO Inferred from Sequence Orthology more info
involved_in vesicle fusion	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in Golgi membrane	IEA Inferred from Electronic Annotation more info
located_in Golgi membrane	ISO Inferred from Sequence Orthology more info
located_in Golgi membrane	TAS Traceable Author Statement more info	PubMed
located_in centrosome	IEA Inferred from Electronic Annotation more info
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in centrosome	ISS Inferred from Sequence or Structural Similarity more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	ISO Inferred from Sequence Orthology more info
part_of heterotrimeric G-protein complex	IBA Inferred from Biological aspect of Ancestor more info
located_in midbody	IEA Inferred from Electronic Annotation more info
located_in midbody	ISO Inferred from Sequence Orthology more info
located_in midbody	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleolus	IEA Inferred from Electronic Annotation more info
located_in nucleolus	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	TAS Traceable Author Statement more info
located_in zymogen granule	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: guanine nucleotide-binding protein G(i) subunit alpha-3

Names: Guanine nucleotide binding, protein, alpha inhibiting polypeptide 3; g(i) alpha-3; guanine nucleotide binding protein (G protein), alpha inhibiting 3; guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3; guanine nucleotide binding protein, alpha inhibiting 3; guanine nucleotide-binding protein G(i) subunit alpha; guanine nucleotide-binding protein G(k) subunit alpha

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_013106.2 → NP_037238.1 guanine nucleotide-binding protein G(i) subunit alpha-3

See identical proteins and their annotated locations for NP_037238.1

Status: PROVISIONAL

Source sequence(s)

JAXUCZ010000002

UniProtKB/Swiss-Prot

P08753

UniProtKB/TrEMBL

A6HUY0, Q45QM8

Conserved Domains (1) summary

cd00066
Location:34 → 348

G-alpha; Alpha subunit of G proteins (guanine nucleotide binding)