APY1 apyrase 1 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: APY1
Official Full Name: apyrase 1
Primary source: TAIR:AT3G04080
Locus tag: AT3G04080
See related: Araport:AT3G04080
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: apyrase 1; APYRASE 1; ATAPY1; T6K12.30; T6K12_30
Summary: Encodes an Golgi-localized integral membrane enzyme with nucleoside diphosphate activity that when mutated in combination with ATAPY2 causes a complete inhibition of pollen germination.
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Genomic context

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See APY1 in Genome Data Viewer

Location:: chromosome: 3

Exon count:: 9

Sequence:: Chromosome: 3; NC_003074.8 (1067558..1071438, complement)

Chromosome 3 - NC_003074.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

APYRASE1/2 mediate red light-induced de-etiolation growth in Arabidopsis seedlings.
Title: APYRASE1/2 mediate red light-induced de-etiolation growth in Arabidopsis seedlings.
Study reports that APY1 and, to a lesser extent, APY2 help control root skewing, the right-slanted growth of roots, in Arabidopsis.
Title: Modulation of Root Skewing in Arabidopsis by Apyrases and Extracellular ATP.
low levels of apyrase chemical inhibitors can induce stomatal opening in the dark, while apyrase enzyme blocks abscissic acid-induced stomatal closure.
Title: Effects of chemical inhibitors and apyrase enzyme further document a role for apyrases and extracellular ATP in the opening and closing of stomates in Arabidopsis.
APY1, APY2, and extracellular ATP play important roles in the signaling steps that link biotic stresses to plant defense responses and growth changes.
Title: Apyrase suppression raises extracellular ATP levels and induces gene expression and cell wall changes characteristic of stress responses.
Suppression of APY1/APY2 expression results in a significant inhibition of polar auxin transport in Arabidopsis hypocotyls and roots.
Title: Role for apyrases in polar auxin transport in Arabidopsis.
AtAPY1 is an integral Golgi protein with the substrate specificity typical for Golgi apyrases.
Title: The Arabidopsis apyrase AtAPY1 is localized in the Golgi instead of the extracellular space.
AtAPY1 and AtAPY2 are Golgi-localized nucleotide diphosphatases and are likely to have roles in regulating UDP/GDP concentrations in the Golgi lumen.
Title: AtAPY1 and AtAPY2 function as Golgi-localized nucleoside diphosphatases in Arabidopsis thaliana.
Both short-term inhibition of ectoapyrase activity by polyclonal antibodies and long-term suppression of APY1 and APY2 transcript levels significantly disrupt normal stomatal behavior in light.
Title: Extracellular nucleotides and apyrases regulate stomatal aperture in Arabidopsis.
acts to reduce the concentration of extracellular nucleotides, highest expression in rapidly growing tissues and/or tissues that accumulate high auxin levels
Title: Apyrases (nucleoside triphosphate-diphosphohydrolases) play a key role in growth control in Arabidopsis.

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General gene information

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NM_111279.5

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables ATP hydrolysis activity	IDA Inferred from Direct Assay more info
enables GDP phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables UDP phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables apyrase activity	IEA Inferred from Electronic Annotation more info
enables calmodulin binding	IDA Inferred from Direct Assay more info
enables nucleoside diphosphate phosphatase activity	IDA Inferred from Direct Assay more info
enables nucleoside diphosphate phosphatase activity	IGI Inferred from Genetic Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within pollen germination	IGI Inferred from Genetic Interaction more info	PubMed
NOT involved_in purinergic nucleotide receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	HDA more info	PubMed
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in Golgi medial cisterna	HDA more info	PubMed
located_in cytoplasm	ISM Inferred from Sequence Model more info
located_in endosome	HDA more info	PubMed
NOT located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed
NOT located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in trans-Golgi network	HDA more info	PubMed

General protein information

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Preferred Names: apyrase 1

NP_187058.1

apyrase 1 (APY1); CONTAINS InterPro DOMAIN/s: Nucleoside phosphatase GDA1/CD39 (InterPro:IPR000407); BEST Arabidopsis thaliana protein match is: apyrase 2 (TAIR:AT5G18280.1); Has 1435 Blast hits to 1431 proteins in 229 species: Archae - 0; Bacteria - 35; Metazoa - 605; Fungi - 313; Plants - 348; Viruses - 0; Other Eukaryotes - 134 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003074.8 Reference assembly

Range

1067558..1071438 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_111279.5 → NP_187058.1 apyrase 1 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_187058.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9SQG2

UniProtKB/TrEMBL

A0A178VED1

Conserved Domains (1) summary

cl17037
Location:70 → 470

NBD_sugar-kinase_HSP70_actin; Nucleotide-Binding Domain of the sugar kinase/HSP70/actin superfamily