U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    NCED3 nine-cis-epoxycarotenoid dioxygenase 3 [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 820667, updated on 18-Sep-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    NCED3
    Official Full Name
    nine-cis-epoxycarotenoid dioxygenase 3
    Primary source
    TAIR:AT3G14440
    Locus tag
    AT3G14440
    See related
    Araport:AT3G14440
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    9-CIS-EPOXYCAROTENOID DIOXYGENASE; ATNCED3; nine-cis-epoxycarotenoid dioxygenase 3; SALT TOLERANT 1; SIS7; STO1; SUGAR INSENSITIVE 7
    Summary
    Encodes 9-<i>cis</i>-epoxycarotenoid dioxygenase, a key enzyme in the biosynthesis of abscisic acid. Regulated in response to drought and salinity. Expressed in roots, flowers and seeds. Localized to the chloroplast stroma and thylakoid membrane.
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    See NCED3 in Genome Data Viewer
    Location:
    chromosome: 3
    Exon count:
    1
    Sequence:
    Chromosome: 3; NC_003074.8 (4831286..4834015, complement)

    Chromosome 3 - NC_003074.8Genomic Context describing neighboring genes Neighboring gene Aldolase-type TIM barrel family protein Neighboring gene Aldolase-type TIM barrel family protein Neighboring gene GRIP/coiled-coil protein Neighboring gene uncharacterized protein Neighboring gene CTC-interacting domain 9

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_003074.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Overexpression of AtSTO1 leads to improved salt tolerance in Populus tremula × P. alba. Lawson SS, et al. Transgenic Res, 2014 Oct. PMID 24929937
    2. Uncoupling the effects of abscisic acid on plant growth and water relations. Analysis of sto1/nced3, an abscisic acid-deficient but salt stress-tolerant mutant in Arabidopsis. Ruggiero B, et al. Plant Physiol, 2004 Oct. PMID 15466233, Free PMC Article
    3. Characterization of the promoter region of an Arabidopsis gene for 9-cis-epoxycarotenoid dioxygenase involved in dehydration-inducible transcription. Behnam B, et al. DNA Res, 2013 Aug. PMID 23604098, Free PMC Article
    4. Involvement of endogenous abscisic acid in methyl jasmonate-induced stomatal closure in Arabidopsis. Hossain MA, et al. Plant Physiol, 2011 May. PMID 21402795, Free PMC Article
    5. Nucleotide variation in the NCED3 region of Arabidopsis thaliana and its association study with abscisic acid content under drought stress. Hao GP, et al. J Integr Plant Biol, 2009 Feb. PMID 19200156

    See all (20) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. results show that de novo biosynthesis of abscisic acid, via up-regulation of the rate-limiting NCED3 gene, can be triggered by changes in leaf turgor; AHK1 does not function as the critical turgor-sensing receptor
      Title: Up-regulation of NCED3 and ABA biosynthesis occur within minutes of a decrease in leaf turgor but AHK1 is not required.
    2. In wild-type leaves, methyl jasmonate (MeJA)stimulates the expression of NCED3, which encodes an enzyme in abscisic acid (ABA) biosynthesis. Endogenous ABA could be involved in MeJA signal transduction and lead to stomatal closure in the guard cells.
      Title: Involvement of endogenous abscisic acid in methyl jasmonate-induced stomatal closure in Arabidopsis.
    3. Overexpression of AtSTO1 improved salt tolerance in transgenic poplar.
      Title: Overexpression of AtSTO1 leads to improved salt tolerance in Populus tremula × P. alba.
    4. A distal ABA responsive element in AtNCED3 promoter is required for positive feedback regulation of ABA biosynthesis in Arabidopsis.
      Title: A distal ABA responsive element in AtNCED3 promoter is required for positive feedback regulation of ABA biosynthesis in Arabidopsis.
    5. An overlapping G-box recognition sequence (50-CACGTG-30) at 22248 base pairs from the transcriptional start site of AtNCED3 is an important cis-acting element in the induction of the dehydration response.
      Title: Characterization of the promoter region of an Arabidopsis gene for 9-cis-epoxycarotenoid dioxygenase involved in dehydration-inducible transcription.
    6. The ced1 (for 9-cis epoxycarotenoid dioxygenase defective 1) mutant isolated in this study showed markedly reduced expression of NCED3 in response to osmotic stress (polyethylene glycol) treatments compared with the wild type.
      Title: The plant cuticle is required for osmotic stress regulation of abscisic acid biosynthesis and osmotic stress tolerance in Arabidopsis.
    7. NCED5 thus contributes, together with NCED3, to abscisic acid production affecting plant growth and water stress tolerance.
      Title: Epoxycarotenoid cleavage by NCED5 fine-tunes ABA accumulation and affects seed dormancy and drought tolerance with other NCED family members.
    8. ATX1 (histone-lysine N-methyltransferase) directly binds NCED3 and regulates its transcription.
      Title: The Arabidopsis trithorax-like factor ATX1 functions in dehydration stress responses via ABA-dependent and ABA-independent pathways.
    9. Studies indicate that NCED3 encodes 9-cis-epoxycarotenoid dioxygenase that catalyzes the oxidative cleavage of a 9-cis isomer of epoxycarotenoid (9-cis-violaxanthin or 9'-cis-neoxanthin) to form xanthoxin.
      Title: Genetic analyses of interactions among gibberellin, abscisic acid, and brassinosteroids in the control of flowering time in Arabidopsis thaliana.
    10. The 274th site (P<-->S) and the 327th site (P<-->R) amino acid variations might be the cause of abscisic acid content increase of 163av accession under drought stress.
      Title: Nucleotide variation in the NCED3 region of Arabidopsis thaliana and its association study with abscisic acid content under drought stress.
    Submit: New GeneRIF Correction See all GeneRIFs (13)

    Pathways from PubChem

    Go to the top of the page

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help
    NM_112304.3

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables 9-cis-epoxycarotenoid dioxygenase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 9-cis-epoxycarotenoid dioxygenase activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in abscisic acid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within abscisic acid biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within abscisic acid biosynthetic process ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    acts_upstream_of_or_within hyperosmotic salinity response IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to osmotic stress IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to water deprivation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in chloroplast ISM
    Inferred from Sequence Model
    more info
    		  
    located_in chloroplast stroma IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in chloroplast thylakoid membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    nine-cis-epoxycarotenoid dioxygenase 3
    NP_188062.1
    • nine-cis-epoxycarotenoid dioxygenase 3 (NCED3); CONTAINS InterPro DOMAIN/s: Carotenoid oxygenase (InterPro:IPR004294); BEST Arabidopsis thaliana protein match is: nine-cis-epoxycarotenoid dioxygenase 9 (TAIR:AT1G78390.1); Has 2945 Blast hits to 2901 proteins in 493 species: Archae - 16; Bacteria - 796; Metazoa - 281; Fungi - 204; Plants - 893; Viruses - 0; Other Eukaryotes - 755 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003074.8 Reference assembly

      Range
      4831286..4834015 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_112304.3 → NP_188062.1  nine-cis-epoxycarotenoid dioxygenase 3 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_188062.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q93ZU5, Q9LRR7
      UniProtKB/TrEMBL
      A0A178VEZ7
      Conserved Domains (1) summary
      PLN02258
      Location:10 → 598
      PLN02258; 9-cis-epoxycarotenoid dioxygenase NCED
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9LRR7.1

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Molecular Biology Databases
    Research Materials
    Tools