U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    CCD8 carotenoid cleavage dioxygenase 8 [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 829417, updated on 18-Sep-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    CCD8
    Official Full Name
    carotenoid cleavage dioxygenase 8
    Primary source
    TAIR:AT4G32810
    Locus tag
    AT4G32810
    See related
    Araport:AT4G32810
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    ATCCD8; carotenoid cleavage dioxygenase 8; MAX4; MORE AXILLARY BRANCHING 4; T16I18.20; T16I18_20
    Summary
    Encodes a protein with similarity to carotenoid cleaving deoxygenases, the enzymes that cleave beta-carotene. Involved in the production of a graft transmissable signal to suppress axillary branching. Protein is localized to chloroplast stroma and expressed primarily in root tip. Mutants in the gene exhibit increased shoot branching, and light-dependent defects in hook opening and hypocotyl/root elongation. Only upregulated by auxin in the root and hypocotyl, and this is not required for the inhibition of shoot branching.
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    See CCD8 in Genome Data Viewer
    Location:
    chromosome: 4
    Exon count:
    5
    Sequence:
    Chromosome: 4; NC_003075.7 (15828228..15831492)

    Chromosome 4 - NC_003075.7Genomic Context describing neighboring genes Neighboring gene miscRNA Neighboring gene Integrase-type DNA-binding superfamily protein Neighboring gene Tetratricopeptide repeat (TPR)-like superfamily protein Neighboring gene ataurora1 Neighboring gene phosphofructokinase 6 Neighboring gene nuclear poly(a) polymerase

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_003075.7

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Knockout of VvCCD8 gene in grapevine affects shoot branching. Ren C, et al. BMC Plant Biol, 2020 Jan 29. PMID 31996144, Free PMC Article
    2. Carotenoid oxygenases involved in plant branching catalyse a highly specific conserved apocarotenoid cleavage reaction. Alder A, et al. Biochem J, 2008 Dec 1. PMID 18637791
    3. MAX4 and RMS1 are orthologous dioxygenase-like genes that regulate shoot branching in Arabidopsis and pea. Sorefan K, et al. Genes Dev, 2003 Jun 15. PMID 12815068, Free PMC Article
    4. Strigolactone Regulates Leaf Senescence in Concert with Ethylene in Arabidopsis. Ueda H, et al. Plant Physiol, 2015 Sep. PMID 25979917, Free PMC Article
    5. Germination stimulants of Phelipanche ramosa in the rhizosphere of Brassica napus are derived from the glucosinolate pathway. Auger B, et al. Mol Plant Microbe Interact, 2012 Jul. PMID 22414435

    See all (11) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. VVitis vinifera orthologue of Arabidopsis ccd8 grapevine shoot branching
      Title: Knockout of VvCCD8 gene in grapevine affects shoot branching.
    2. The strigolactone biosynthesis genes MAX3 and MAX4 were drastically induced during dark incubation and treatment with the senescence-promoting phytohormone ethylene, suggesting that strigolactone is synthesized in the leaf during leaf senescence.
      Title: Strigolactone Regulates Leaf Senescence in Concert with Ethylene in Arabidopsis.
    3. Wild-type and strigolactone mutants of A. thaliana Atccd7 and Atccd8 induced similar levels of P. ramosa seed germination, suggesting that compounds other than strigolactone function as germination stimulants for P. ramosa in other Brassicaceae spp.
      Title: Germination stimulants of Phelipanche ramosa in the rhizosphere of Brassica napus are derived from the glucosinolate pathway.
    4. Results show that MAX3 and MAX4 genes were down-regulated in igi1 (Inflorescence Growth Inhibitor 1) mutants.
      Title: Over-expression of the IGI1 leading to altered shoot-branching development related to MAX pathway in Arabidopsis.
    5. activities in vitro using naturally accumulated carotenoids and synthetic apocarotenoid substrates
      Title: Carotenoid oxygenases involved in plant branching catalyse a highly specific conserved apocarotenoid cleavage reaction.
    6. the sequential cleavages of beta-carotene by AtCCD7 and AtCCD8 may be initial steps in the synthesis of a carotenoid-derived signaling molecule necessary for the regulation lateral branching. [AtCCD8]
      Title: The biochemical characterization of two carotenoid cleavage enzymes from Arabidopsis indicates that a carotenoid-derived compound inhibits lateral branching.
    Submit: New GeneRIF Correction

    Pathways from PubChem

    Go to the top of the page

    General gene information

    Go to the top of the page Help

    Homology

    NM_119434.4

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables 9-cis-10'-apo-beta-carotenal cleavage oxygenase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables all-trans-beta-apo-10'-carotenal cleavage oxygenase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    Process Evidence Code Pubs
    NOT involved_in auxin metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within auxin polar transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within carotene catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in carotene catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within leaf morphogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to auxin IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within secondary shoot formation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within secondary shoot formation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in strigolactone biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within xanthophyll catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in chloroplast ISM
    Inferred from Sequence Model
    more info
    		  
    located_in chloroplast stroma IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    carotenoid cleavage dioxygenase 8
    NP_195007.2
    • carotenoid cleavage dioxygenase 8 (CCD8); CONTAINS InterPro DOMAIN/s: Carotenoid oxygenase (InterPro:IPR004294); BEST Arabidopsis thaliana protein match is: nine-cis-epoxycarotenoid dioxygenase 6 (TAIR:AT3G24220.1); Has 2642 Blast hits to 2570 proteins in 479 species: Archae - 17; Bacteria - 769; Metazoa - 368; Fungi - 139; Plants - 827; Viruses - 0; Other Eukaryotes - 522 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003075.7 Reference assembly

      Range
      15828228..15831492
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001342177.1 → NP_001329787.1  carotenoid cleavage dioxygenase 8 [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A178V3Z4, A0A1P8B8T4

    2. NM_119434.4 → NP_195007.2  carotenoid cleavage dioxygenase 8 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_195007.2

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q8VY26, Q9M079
      UniProtKB/TrEMBL
      A0A178V3Z4, A0A5S9XY27, A0A654FUY3
      Conserved Domains (1) summary
      pfam03055
      Location:92 → 568
      RPE65; Retinal pigment epithelial membrane protein
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q8VY26.1

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Other Literature Sources
    Molecular Biology Databases
    Research Materials
    Tools