UNE5 thioredoxin family protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: UNE5
Official Full Name: thioredoxin family protein
Primary source: TAIR:AT2G47470
Locus tag: AT2G47470
See related: Araport:AT2G47470
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ARABIDOPSIS THALIANA PROTEIN DISULFIDE ISOMERASE 11; ATPDI11; ATPDIL2-1; MATERNAL EFFECT EMBRYO ARREST 30; MEE30; PDI-LIKE 2-1; PDI11; PROTEIN DISULFIDE ISOMERASE 11; T30B22.23; UNFERTILIZED EMBRYO SAC 5
Summary: Encodes a protein disulfide isomerase-like (PDIL) protein, a member of a multigene family within the thioredoxin (TRX) superfamily. Transcript levels for this gene are up-regulated in response to three different chemical inducers of ER stress (dithiothreitol, beta-mercaptoethanol, and tunicamycin). AtIRE1-2 does not appear to be required for this response, but the atbzip60 mutant has a diminished response.
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Genomic context

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See UNE5 in Genome Data Viewer

Location:: chromosome: 2

Exon count:: 10

Sequence:: Chromosome: 2; NC_003071.7 (19481254..19484015)

Chromosome 2 - NC_003071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003071.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Expression Characterization of AtPDI11 and Functional Analysis of AtPDI11 D Domain in Oxidative Protein Folding.
Title: Expression Characterization of AtPDI11 and Functional Analysis of AtPDI11 D Domain in Oxidative Protein Folding.
Arabidopsis PDI11 interacts with lectin molecular chaperons calreticulin 1 and 2 through its D domain.
Title: Arabidopsis PDI11 interacts with lectin molecular chaperons calreticulin 1 and 2 through its D domain.
In this study, we demonstrated that AtPDI11 has oxidoreductase activity in vitro using a GSSG/GSH-mediated oxidative protein folding system. Moreover, the active site in the a' domain of AtPDI11 is critical for its oxidative folding activity.
Title: Characterization of the oxidative protein folding activity of a unique plant oxidoreductase, Arabidopsis protein disulfide isomerase-11.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

DXMit3 (e-PCR), detects polymorphism
- UniSTS:239878

D11S2920 (e-PCR)
- UniSTS:152073

D11S2591 (e-PCR)
- UniSTS:18383

Homology

Orthologs from OrthoDB

NM_001036478.2

Supported by ESTs:

NM_001084605.1

Supported by ESTs:

NM_130315.5

Supported by ESTs:

NM_201979.2

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables protein disulfide isomerase activity	IEA Inferred from Electronic Annotation more info
enables protein disulfide isomerase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within double fertilization forming a zygote and endosperm	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within embryo development ending in seed dormancy	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within embryo sac development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within pollen tube development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to endoplasmic reticulum stress	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
NOT located_in Golgi apparatus	RCA inferred from Reviewed Computational Analysis more info	PubMed
located_in chloroplast	ISM Inferred from Sequence Model more info
located_in cytosol	HDA more info	PubMed
NOT located_in cytosol	RCA inferred from Reviewed Computational Analysis more info	PubMed
located_in endoplasmic reticulum	HDA more info	PubMed
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in extracellular region	ISM Inferred from Sequence Model more info
located_in plant-type cell wall	HDA more info	PubMed
located_in plant-type vacuole	HDA more info	PubMed
located_in secretory vesicle	HDA more info	PubMed

General protein information

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Preferred Names: thioredoxin family protein

NP_001031555.1

UNFERTILIZED EMBRYO SAC 5 (UNE5); FUNCTIONS IN: protein disulfide isomerase activity; INVOLVED IN: in 6 processes; LOCATED IN: endoplasmic reticulum, plasma membrane, plant-type cell wall; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Thioredoxin fold (InterPro:IPR012335), Disulphide isomerase (InterPro:IPR005788), Thioredoxin domain (InterPro:IPR013766), Endoplasmic reticulum, protein ERp29, C-terminal (InterPro:IPR011679), Thioredoxin, conserved site (InterPro:IPR017937), Thioredoxin-like subdomain (InterPro:IPR006662), Thioredoxin-like (InterPro:IPR017936), Thioredoxin-like fold (InterPro:IPR012336); BEST Arabidopsis thaliana protein match is: PDI-like 2-2 (TAIR:AT1G04980.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).

NP_001078074.1

UNFERTILIZED EMBRYO SAC 5 (UNE5); FUNCTIONS IN: protein disulfide isomerase activity; INVOLVED IN: response to endoplasmic reticulum stress, double fertilization forming a zygote and endosperm, embryo sac development, pollen tube development, embryo development ending in seed dormancy; LOCATED IN: endoplasmic reticulum, plasma membrane, plant-type cell wall; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Thioredoxin fold (InterPro:IPR012335), Disulphide isomerase (InterPro:IPR005788), Thioredoxin domain (InterPro:IPR013766), Endoplasmic reticulum, protein ERp29, C-terminal (InterPro:IPR011679), Thioredoxin, conserved site (InterPro:IPR017937), Thioredoxin-like subdomain (InterPro:IPR006662), Thioredoxin-like (InterPro:IPR017936), Thioredoxin-like fold (InterPro:IPR012336); BEST Arabidopsis thaliana protein match is: PDI-like 2-2 (TAIR:AT1G04980.1); Has 37639 Blast hits to 19288 proteins in 2969 species: Archae - 403; Bacteria - 17566; Metazoa - 6414; Fungi - 2106; Plants - 3416; Viruses - 32; Other Eukaryotes - 7702 (source: NCBI BLink).

NP_182269.1

UNFERTILIZED EMBRYO SAC 5 (UNE5); FUNCTIONS IN: protein disulfide isomerase activity; INVOLVED IN: in 6 processes; LOCATED IN: endoplasmic reticulum, plasma membrane, plant-type cell wall; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Thioredoxin fold (InterPro:IPR012335), Disulphide isomerase (InterPro:IPR005788), Thioredoxin domain (InterPro:IPR013766), Endoplasmic reticulum, protein ERp29, C-terminal (InterPro:IPR011679), Thioredoxin, conserved site (InterPro:IPR017937), Thioredoxin-like subdomain (InterPro:IPR006662), Thioredoxin-like (InterPro:IPR017936), Thioredoxin-like fold (InterPro:IPR012336); BEST Arabidopsis thaliana protein match is: PDI-like 2-2 (TAIR:AT1G04980.1); Has 37720 Blast hits to 19404 proteins in 2983 species: Archae - 403; Bacteria - 17657; Metazoa - 6433; Fungi - 2162; Plants - 3420; Viruses - 32; Other Eukaryotes - 7613 (source: NCBI BLink).

NP_973708.1

UNFERTILIZED EMBRYO SAC 5 (UNE5); FUNCTIONS IN: protein disulfide isomerase activity; INVOLVED IN: in 6 processes; LOCATED IN: endoplasmic reticulum, plasma membrane, plant-type cell wall; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Thioredoxin fold (InterPro:IPR012335), Disulphide isomerase (InterPro:IPR005788), Thioredoxin domain (InterPro:IPR013766), Thioredoxin, conserved site (InterPro:IPR017937), Thioredoxin-like subdomain (InterPro:IPR006662), Thioredoxin-like (InterPro:IPR017936), Thioredoxin-like fold (InterPro:IPR012336); BEST Arabidopsis thaliana protein match is: PDI-like 2-2 (TAIR:AT1G04980.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003071.7 Reference assembly

Range

19481254..19484015

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_130315.5 → NP_182269.1 thioredoxin family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_182269.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O22263, Q546R3

UniProtKB/TrEMBL

A0A178W1F8, A0A5S9X7T6, A0A654F2S3

Conserved Domains (2) summary

cd00238
Location:266 → 357

ERp29c; ERp29 and ERp38, C-terminal domain; composed of the protein disulfide isomerase (PDI)-like proteins ERp29 and ERp38. ERp29 (also called ERp28) is a ubiquitous endoplasmic reticulum (ER)-resident protein expressed in high levels in secretory cells. It ...

cd02998
Location:142 → 245

PDI_a_ERp38; PDIa family, endoplasmic reticulum protein 38 (ERp38) subfamily; composed of proteins similar to the P5-like protein first isolated from alfalfa, which contains two redox active TRX (a) domains at the N-terminus, like human P5, and a C-terminal domain ...
NM_201979.2 → NP_973708.1 thioredoxin family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_973708.1

Status: REVIEWED

UniProtKB/TrEMBL

F4IL53

Conserved Domains (2) summary

cd02998
Location:142 → 245

PDI_a_ERp38; PDIa family, endoplasmic reticulum protein 38 (ERp38) subfamily; composed of proteins similar to the P5-like protein first isolated from alfalfa, which contains two redox active TRX (a) domains at the N-terminus, like human P5, and a C-terminal domain ...

pfam13848
Location:56 → 247

Thioredoxin_6; Thioredoxin-like domain
NM_001036478.2 → NP_001031555.1 thioredoxin family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001031555.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A654F2S3, F4IL52

Conserved Domains (3) summary

cd02998
Location:142 → 245

PDI_a_ERp38; PDIa family, endoplasmic reticulum protein 38 (ERp38) subfamily; composed of proteins similar to the P5-like protein first isolated from alfalfa, which contains two redox active TRX (a) domains at the N-terminus, like human P5, and a C-terminal domain ...

pfam13848
Location:56 → 247

Thioredoxin_6; Thioredoxin-like domain

cl02782
Location:266 → 305

ERp29c; ERp29 and ERp38, C-terminal domain; composed of the protein disulfide isomerase (PDI)-like proteins ERp29 and ERp38. ERp29 (also called ERp28) is a ubiquitous endoplasmic reticulum (ER)-resident protein expressed in high levels in secretory cells. It ...
NM_001084605.1 → NP_001078074.1 thioredoxin family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001078074.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A654F2S3, A8MSC5

Conserved Domains (2) summary

cd02998
Location:142 → 245

PDI_a_ERp38; PDIa family, endoplasmic reticulum protein 38 (ERp38) subfamily; composed of proteins similar to the P5-like protein first isolated from alfalfa, which contains two redox active TRX (a) domains at the N-terminus, like human P5, and a C-terminal domain ...

pfam07749
Location:266 → 331

ERp29; Endoplasmic reticulum protein ERp29, C-terminal domain