PCAP1 plasma-membrane associated cation-binding protein 1 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: PCAP1
Official Full Name: plasma-membrane associated cation-binding protein 1
Primary source: TAIR:AT4G20260
Locus tag: AT4G20260
See related: Araport:AT4G20260
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ARABIDOPSIS THALIANA PLASMA-MEMBRANE ASSOCIATED CATION-BINDING PROTEIN 1; ATPCAP1; F1C12.180; F1C12_180; MDP25; microtubule-destabilizing protein 25; plasma-membrane associated cation-binding protein 1
Summary: Encodes a Ca2+ and Cu2+ binding protein. N-terminal myristylation on glycine 2 appears to enable it to associate tightly with the plasma membrane. Recombinant PCaP1 interacts strongly with phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) and PtdIns (3,4,5)P3, and weakly with PtdIns(3,5)P2 and PtdIns(4,5). It also interacts with calmodulin (CaM) in a calcium-dependent manner. CaM does not interfere with PCaP1 membrane localization but does weaken interactions between it and the PtdInsPs. PCaP1 has an apparent Kd of 10 uM for Cu2+ and can bind six ions per protein. Transcript levels for PCaP1 first fall and then rise following exposure to CuCl2. Mannitol, sorbitol, and the flg22 oligopeptide also increase expression levels.
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Genomic context

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See PCAP1 in Genome Data Viewer

Location:: chromosome: 4

Exon count:: 5

Sequence:: Chromosome: 4; NC_003075.7 (10940414..10943561)

Chromosome 4 - NC_003075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003075.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The plasma membrane-associated cation-binding protein PCaP1 of Arabidopsis thaliana is a uranyl-binding protein.
Title: The plasma membrane-associated cation-binding protein PCaP1 of Arabidopsis thaliana is a uranyl-binding protein.
The plasma membrane-associated Ca(2+) -binding protein, PCaP1, is required for oligogalacturonide and flagellin-induced priming and immunity.
Title: The plasma membrane-associated Ca(2+) -binding protein, PCaP1, is required for oligogalacturonide and flagellin-induced priming and immunity.
contributes to hydrotropism through a MIZ1-independent pathway or as one of the upstream components that transduce water potential signals to MIZ1
Title: Plasma Membrane-Associated Ca2+-Binding Protein PCaP1 is Involved in Root Hydrotropism of Arabidopsis thaliana.
PCaP1 is involved in the stomatal movement, especially closure process, in leaves and response to excessive copper in root and leaf as a mineral nutrient as a physiological role.
Title: A novel-type phosphatidylinositol phosphate-interactive, Ca-binding protein PCaP1 in Arabidopsis thaliana: stable association with plasma membrane and partial involvement in stomata closure.
MICROTUBULE-DESTABILIZING PROTEIN25 (MDP25) has the actin filament-severing activity of an actin binding protein.
Title: Arabidopsis microtubule-destabilizing protein 25 functions in pollen tube growth by severing actin filaments.
MDP25 destabilizes microtubules and exerts its effect on microtubules as a result of transient elevation of cytosolic calcium levels. [MDP25]
Title: Characterization of the role of calcium in regulating the microtubule-destabilizing activity of MDP25.
potyviral P3N-PIPO interacts specifically with the host plasma membrane protein PCaP1 to participate in cell-to-cell movement.
Title: Interaction of the trans-frame potyvirus protein P3N-PIPO with host protein PCaP1 facilitates potyvirus movement.
MDP25 induces microtubule depolymerization.
Title: MDP25, a novel calcium regulatory protein, mediates hypocotyl cell elongation by destabilizing cortical microtubules in Arabidopsis.
PCaP1 has a high Cu(2+)-binding capacity with a relatively high affinity. PCaP1 lacks cysteine and histidine residues. A large number of glutamate residues may be involved in the Cu(2+) binding. [PCaP1 cation-binding protein]
Title: A plasma membrane-associated protein of Arabidopsis thaliana AtPCaP1 binds copper ions and changes its higher order structure.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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NM_001036599.2

Supported by ESTs:

NM_001036600.2

Supported by ESTs:

NM_001203849.1

Supported by ESTs:

NM_001203850.1

Supported by ESTs:

NM_001341407.1

Supported by ESTs:

NM_001341408.1

Supported by ESTs:

NM_001341409.1

Supported by ESTs:

NM_001341410.1

Supported by ESTs:

NM_118145.4

Supported by ESTs:

NM_179081.5

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables calcium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables calmodulin binding	IDA Inferred from Direct Assay more info	PubMed
enables copper ion binding	IDA Inferred from Direct Assay more info	PubMed
enables microtubule binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-3,4,5-trisphosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-3,4-bisphosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-3,5-bisphosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-4,5-bisphosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in N-terminal protein myristoylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to copper ion	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to iron ion	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to magnesium ion	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to magnesium starvation	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to mannitol stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to molecule of bacterial origin	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to potassium ion	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to salt stress	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to sorbitol	IEP Inferred from Expression Pattern more info	PubMed
involved_in cortical microtubule organization	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cortical microtubule organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within intracellular transport of virus	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of microtubule polymerization	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of unidimensional cell growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of unidimensional cell growth	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of microtubule depolymerization	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of microtubule depolymerization	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within response to calcium ion	IDA Inferred from Direct Assay more info	PubMed
NOT involved_in response to cold	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to cold	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to water deprivation	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within stomatal closure	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasmic microtubule	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	HDA more info	PubMed
located_in plant-type vacuole	HDA more info	PubMed
located_in plasma membrane	HDA more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasmodesma	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: plasma-membrane associated cation-binding protein 1

NP_001031676.1

plasma-membrane associated cation-binding protein 1 (PCAP1); FUNCTIONS IN: calmodulin binding, copper ion binding, phosphatidylinositol-3,4,5-trisphosphate binding, phosphatidylinositol-3,5-bisphosphate binding, calcium ion binding; INVOLVED IN: response to cold, N-terminal peptidyl-glycine N-myristoylation, defense response to bacterium; LOCATED IN: plasma membrane, vacuole; EXPRESSED IN: 35 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: DREPP plasma membrane polypeptide (InterPro:IPR008469); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).

NP_001031677.1

plasma-membrane associated cation-binding protein 1 (PCAP1); FUNCTIONS IN: calmodulin binding, copper ion binding, phosphatidylinositol-3,4,5-trisphosphate binding, phosphatidylinositol-3,5-bisphosphate binding, calcium ion binding; INVOLVED IN: response to cold, N-terminal peptidyl-glycine N-myristoylation, defense response to bacterium; LOCATED IN: plasma membrane, vacuole; EXPRESSED IN: 35 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: DREPP plasma membrane polypeptide (InterPro:IPR008469); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).

NP_001190778.1

plasma-membrane associated cation-binding protein 1 (PCAP1); FUNCTIONS IN: calmodulin binding, copper ion binding, phosphatidylinositol-3,4,5-trisphosphate binding, phosphatidylinositol-3,5-bisphosphate binding, calcium ion binding; INVOLVED IN: response to cold, N-terminal peptidyl-glycine N-myristoylation; LOCATED IN: plasma membrane; EXPRESSED IN: 34 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: DREPP plasma membrane polypeptide (InterPro:IPR008469).

NP_001190779.1

plasma-membrane associated cation-binding protein 1 (PCAP1); FUNCTIONS IN: calmodulin binding, copper ion binding, phosphatidylinositol-3,4,5-trisphosphate binding, phosphatidylinositol-3,5-bisphosphate binding, calcium ion binding; INVOLVED IN: response to cold, N-terminal peptidyl-glycine N-myristoylation; LOCATED IN: plasma membrane; EXPRESSED IN: 34 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: DREPP plasma membrane polypeptide (InterPro:IPR008469).

NP_001320005.1

plasma-membrane associated cation-binding protein 1 (PCAP1); FUNCTIONS IN: calmodulin binding, copper ion binding, phosphatidylinositol-3,4,5-trisphosphate binding, phosphatidylinositol-3,5-bisphosphate binding, calcium ion binding; INVOLVED IN: response to cold, N-terminal peptidyl-glycine N-myristoylation, defense response to bacterium; LOCATED IN: plasma membrane, chloroplast, vacuole; EXPRESSED IN: 35 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: DREPP plasma membrane polypeptide (InterPro:IPR008469); Has 6211 Blast hits to 3706 proteins in 536 species: Archae - 35; Bacteria - 949; Metazoa - 1704; Fungi - 697; Plants - 277; Viruses - 59; Other Eukaryotes - 2490 (source: NCBI BLink).

NP_193759.1

plasma-membrane associated cation-binding protein 1 (PCAP1); FUNCTIONS IN: calmodulin binding, copper ion binding, phosphatidylinositol-3,4,5-trisphosphate binding, phosphatidylinositol-3,5-bisphosphate binding, calcium ion binding; INVOLVED IN: response to cold, N-terminal peptidyl-glycine N-myristoylation, defense response to bacterium; LOCATED IN: plasma membrane, chloroplast, vacuole; EXPRESSED IN: 35 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: DREPP plasma membrane polypeptide (InterPro:IPR008469); Has 6213 Blast hits to 3707 proteins in 537 species: Archae - 35; Bacteria - 951; Metazoa - 1704; Fungi - 697; Plants - 277; Viruses - 59; Other Eukaryotes - 2490 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003075.7 Reference assembly

Range

10940414..10943561

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001341407.1 → NP_001320005.1 plasma-membrane associated cation-binding protein 1 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/Swiss-Prot

F4JUT4, Q56YV4, Q8H7H8, Q8LDH5, Q96262

UniProtKB/TrEMBL

A0A5S9XU81, A0A654FR46, A0A7G2F429
NM_001341408.1 → NP_001329494.1 plasma-membrane associated cation-binding protein 1 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/Swiss-Prot

F4JUT4, Q56YV4, Q8H7H8, Q8LDH5, Q96262

UniProtKB/TrEMBL

A0A5S9XU81, A0A654FR46, A0A7G2F429
NM_001341409.1 → NP_001329493.1 plasma-membrane associated cation-binding protein 1 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/Swiss-Prot

F4JUT4, Q56YV4, Q8H7H8, Q8LDH5, Q96262

UniProtKB/TrEMBL

A0A5S9XU81, A0A654FR46, A0A7G2F429
NM_001341410.1 → NP_001329492.1 plasma-membrane associated cation-binding protein 1 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/Swiss-Prot

F4JUT4, Q56YV4, Q8H7H8, Q8LDH5, Q96262

UniProtKB/TrEMBL

A0A5S9XU81, A0A654FR46, A0A7G2F429
NM_179081.5 → NP_849412.1 plasma-membrane associated cation-binding protein 1 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_849412.1

Status: REVIEWED

UniProtKB/Swiss-Prot

F4JUT4, Q56YV4, Q8H7H8, Q8LDH5, Q96262

UniProtKB/TrEMBL

A0A5S9XU81, A0A654FR46, A0A7G2F429

Conserved Domains (1) summary

pfam05558
Location:1 → 225

DREPP; DREPP plasma membrane polypeptide
NM_001036600.2 → NP_001031677.1 plasma-membrane associated cation-binding protein 1 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001031677.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A5S9XU81, A0A654FR46, A0A7G2F429

Conserved Domains (1) summary

pfam05558
Location:1 → 226

DREPP; DREPP plasma membrane polypeptide
NM_001036599.2 → NP_001031676.1 plasma-membrane associated cation-binding protein 1 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001031676.1

Status: REVIEWED

UniProtKB/Swiss-Prot

F4JUT4, Q56YV4, Q8H7H8, Q8LDH5, Q96262

UniProtKB/TrEMBL

A0A5S9XU81, A0A654FR46, A0A7G2F429

Conserved Domains (1) summary

pfam05558
Location:1 → 225

DREPP; DREPP plasma membrane polypeptide
NM_001203849.1 → NP_001190778.1 plasma-membrane associated cation-binding protein 1 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001190778.1

Status: REVIEWED

UniProtKB/TrEMBL

F4JUT9

Conserved Domains (1) summary

pfam05558
Location:1 → 143

DREPP; DREPP plasma membrane polypeptide
NM_001203850.1 → NP_001190779.1 plasma-membrane associated cation-binding protein 1 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001190779.1

Status: REVIEWED

UniProtKB/TrEMBL

F4JUT8

Conserved Domains (1) summary

pfam05558
Location:1 → 166

DREPP; DREPP plasma membrane polypeptide
NM_118145.4 → NP_193759.1 plasma-membrane associated cation-binding protein 1 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_193759.1

Status: REVIEWED

UniProtKB/Swiss-Prot

F4JUT4, Q56YV4, Q8H7H8, Q8LDH5, Q96262

UniProtKB/TrEMBL

A0A5S9XU81, A0A654FR46, A0A7G2F429

Conserved Domains (1) summary

pfam05558
Location:1 → 225

DREPP; DREPP plasma membrane polypeptide