Brf Brf RNA polymerase III subunit [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Brfprovided by FlyBase
Official Full Name: Brf RNA polymerase III subunitprovided by FlyBase
Primary source: FLYBASE:FBgn0038499
Locus tag: Dmel_CG31256
See related: AllianceGenome:FB:FBgn0038499
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: brf; BRF; Brf1; BRF1; CG31256; CG4155; CG5419; Dmel\CG31256; TFIIIB
Summary: Enables RNA polymerase III type 3 promoter sequence-specific DNA binding activity and TBP-class protein binding activity. Contributes to core promoter sequence-specific DNA binding activity. Involved in transcription by RNA polymerase III. Part of transcription factor TFIIIB complex. Is expressed in several structures, including central brain primordium; embryonic/larval midgut; embryonic/larval midgut primordium; ganglia; and larval nervous system. Human ortholog(s) of this gene implicated in cerebellofaciodental syndrome. Orthologous to human BRF1 (BRF1 RNA polymerase III transcription initiation factor subunit). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: 90A3-90A5; 3-60 cM

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (17374833..17391337, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (13200555..13217059, complement)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Results indicate that a portion of Brf1 is sandwiched between Bdp1 and TBP upstream of the U6 TATA box. Furthermore, Bdp1 traverses the DNA under the N-terminal stirrup of TBP to interact with the DNA (and very likely Brf1) downstream of the TATA sequence.
Title: TFIIIB subunit locations on U6 gene promoter DNA mapped by site-specific protein-DNA photo-cross-linking.
in Drosophila the alternative TRF1/BRF complex appears responsible for the initiation of all known classes of Pol III transcription
Title: Novel TRF1/BRF target genes revealed by genome-wide analysis of Drosophila Pol III transcription.

Submit: New GeneRIF Correction

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables RNA polymerase III general transcription initiation factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase III type 3 promoter sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase III type 3 promoter sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables TBP-class protein binding	IEA Inferred from Electronic Annotation more info
enables TBP-class protein binding	IPI Inferred from Physical Interaction more info	PubMed
contributes_to core promoter sequence-specific DNA binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA-templated transcription initiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA-templated transcription initiation	IEA Inferred from Electronic Annotation more info
involved_in transcription by RNA polymerase III	IBA Inferred from Biological aspect of Ancestor more info
involved_in transcription by RNA polymerase III	IDA Inferred from Direct Assay more info	PubMed
involved_in transcription by RNA polymerase III	IPI Inferred from Physical Interaction more info	PubMed
involved_in transcription preinitiation complex assembly	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
part_of transcription factor TFIIIB complex	IBA Inferred from Biological aspect of Ancestor more info
part_of transcription factor TFIIIB complex	IDA Inferred from Direct Assay more info	PubMed
part_of transcription preinitiation complex	IBA Inferred from Biological aspect of Ancestor more info
part_of transcription regulator complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: Brf RNA polymerase III subunit

Names: Brf-PA; Brf-PB; CG31256-PA; CG31256-PB; RNA Pol III transcription factor

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

17374833..17391337 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_142359.3 → NP_650616.2 Brf RNA polymerase III subunit, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_650616.2

Status: REVIEWED

UniProtKB/TrEMBL

Q960W2, Q9NG52, Q9VEL2

Related

FBpp0082893

Conserved Domains (5) summary

COG1405
Location:6 → 270

SUA7; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB [Transcription]

cd00043
Location:183 → 268

CYCLIN; Cyclin box fold. Protein binding domain functioning in cell-cycle and transcription control. Present in cyclins, TFIIB and Retinoblastoma (RB).The cyclins consist of 8 classes of cell cycle regulators that regulate cyclin dependent kinases (CDKs). TFIIB ...

pfam07741
Location:441 → 531

BRF1; Brf1-like TBP-binding domain

pfam08271
Location:5 → 44

TF_Zn_Ribbon; TFIIB zinc-binding

cl00050
Location:108 → 164

CYCLIN; Cyclin box fold. Protein binding domain functioning in cell-cycle and transcription control. Present in cyclins, TFIIB and Retinoblastoma (RB).The cyclins consist of 8 classes of cell cycle regulators that regulate cyclin dependent kinases (CDKs). TFIIB ...
NM_001170165.2 → NP_001163636.1 Brf RNA polymerase III subunit, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001163636.1

Status: REVIEWED

UniProtKB/TrEMBL

Q960W2, Q9NG52, Q9VEL2

Conserved Domains (5) summary

COG1405
Location:6 → 270

SUA7; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB [Transcription]

cd00043
Location:183 → 268

CYCLIN; Cyclin box fold. Protein binding domain functioning in cell-cycle and transcription control. Present in cyclins, TFIIB and Retinoblastoma (RB).The cyclins consist of 8 classes of cell cycle regulators that regulate cyclin dependent kinases (CDKs). TFIIB ...

pfam07741
Location:441 → 531

BRF1; Brf1-like TBP-binding domain

pfam08271
Location:5 → 44

TF_Zn_Ribbon; TFIIB zinc-binding

cl00050
Location:108 → 164

CYCLIN; Cyclin box fold. Protein binding domain functioning in cell-cycle and transcription control. Present in cyclins, TFIIB and Retinoblastoma (RB).The cyclins consist of 8 classes of cell cycle regulators that regulate cyclin dependent kinases (CDKs). TFIIB ...