wls wntless [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: wlsprovided by FlyBase
Official Full Name: wntlessprovided by FlyBase
Primary source: FLYBASE:FBgn0036141
Locus tag: Dmel_CG6210
See related: AllianceGenome:FB:FBgn0036141
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG6210; Dmel\CG6210; evi; Evi; Evi/Wls; Spr; srt; Srt; Wls; wls/evi; Wls/Evi
Summary: Enables Wnt-protein binding activity. Involved in several processes, including imaginal disc-derived appendage morphogenesis; protein transport; and regulation of protein transport. Located in several cellular components, including cytoplasmic vesicle; extracellular exosome; and synaptic membrane. Is expressed in several structures, including adult head; anterior endoderm; anterior endoderm anlage; ganglia; and visceral trunk mesoderm derivative. Human ortholog(s) of this gene implicated in Zaki syndrome. Orthologous to human WLS (Wnt ligand secretion mediator). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 68A9-68A9; 3-34 cM

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3L	NT_037436.4 (11166620..11169625, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3L	NT_037436.3 (11159720..11162725, complement)

Chromosome 3L - NT_037436.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Dissociation of Drosophila Evi-Wg Complex Occurs Post Apical Internalization in the Maturing Acidic Endosomes.
Title: Dissociation of Drosophila Evi-Wg Complex Occurs Post Apical Internalization in the Maturing Acidic Endosomes.
MicroRNA-31 Regulates Expression of Wntless in Both Drosophila melanogaster and Human Oral Cancer Cells.
Title: MicroRNA-31 Regulates Expression of Wntless in Both Drosophila melanogaster and Human Oral Cancer Cells.
A cell-autonomous role of the Wnt-secretory factor MIG-14/Wntless was found to mediate dendrite self-avoidance.
Title: Cell-Autonomous Regulation of Dendrite Self-Avoidance by the Wnt Secretory Factor MIG-14/Wntless.
Dysfunction of Wntless triggers the retrograde Golgi-to-ER transport of Wingless and induces ER stress.
Title: Dysfunction of Wntless triggers the retrograde Golgi-to-ER transport of Wingless and induces ER stress.
The uncharacterized Drosophila protein Evi5 was identified as an essential membrane trafficking regulator, and the molecular mechanism by which Evi5 regulates BC migration was described.
Title: Evi5 promotes collective cell migration through its Rab-GAP activity.
Syx1A, Rab11, and its effector Myosin5 were required for proper Evi vesicle release.
Title: Mechanism of evenness interrupted (Evi)-exosome release at synaptic boutons.
DSNX3 regulates Wg secretion via retromer-dependent Wls recycling
Title: SNX3 controls Wingless/Wnt secretion through regulating retromer-dependent recycling of Wntless.
Por-mediated lipidation of the S239-equivalent residue is essential for the interaction with, and secretion by, Wls.
Title: Porcupine-mediated lipidation is required for Wnt recognition by Wls.
These findings uncover a previously unknown cellular mechanism by which a secreted Wnt is transported across synapses by Evi-containing vesicles and reveal trafficking functions of Evi in both the Wnt-producing and the Wnt-receiving cells.
Title: Trans-synaptic transmission of vesicular Wnt signals through Evi/Wntless.
WntD, a recently characterized Drosophila Wnt family member, does not require Porcupine or Wntless/Evi/Sprinter for its secretion or signaling activity
Title: Lipid-independent secretion of a Drosophila Wnt protein.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables Wnt-protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables Wnt-protein binding	IEA Inferred from Electronic Annotation more info
enables Wnt-protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Golgi to plasma membrane protein transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in Wnt protein secretion	IBA Inferred from Biological aspect of Ancestor more info
involved_in Wnt protein secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in Wnt signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in compound eye morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cuticle pattern formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in imaginal disc-derived leg morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in imaginal disc-derived wing margin morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in imaginal disc-derived wing morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular protein transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of Wnt protein secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of Wnt signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of intracellular protein transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in segment polarity determination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in trans-synaptic signaling	IDA Inferred from Direct Assay more info	PubMed
involved_in trans-synaptic signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in trans-synaptic signaling via exosome	IDA Inferred from Direct Assay more info	PubMed
involved_in trans-synaptic signaling via exosome	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in Golgi membrane	IDA Inferred from Direct Assay more info	PubMed
located_in early endosome	IDA Inferred from Direct Assay more info	PubMed
located_in endomembrane system	HDA more info	PubMed
is_active_in endomembrane system	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in endosome membrane	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular exosome	IDA Inferred from Direct Assay more info	PubMed
located_in multivesicular body	IDA Inferred from Direct Assay more info	PubMed
located_in neuromuscular junction	IDA Inferred from Direct Assay more info	PubMed
located_in neuromuscular junction	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in organelle membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in postsynaptic membrane	IDA Inferred from Direct Assay more info	PubMed
located_in presynaptic membrane	IDA Inferred from Direct Assay more info	PubMed
located_in synaptic vesicle membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: wntless

Names: CG6210-PA; CG6210-PB; evenness interrupted; sprinter; wls-PA; wls-PB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_037436.4 Reference assembly

Range

11166620..11169625 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_168450.3 → NP_729681.1 wntless, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_729681.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q95ST2

Related

FBpp0075948

Conserved Domains (1) summary

pfam06664
Location:185 → 507

MIG-14_Wnt-bd; Wnt-binding factor required for Wnt secretion
NM_140188.4 → NP_648445.1 wntless, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_648445.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q95ST2, Q9VTG4

Related

FBpp0075947

Conserved Domains (1) summary

pfam06664
Location:185 → 539

MIG-14_Wnt-bd; Wnt-binding factor required for Wnt secretion