Chrm4 cholinergic receptor, muscarinic 4 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Chrm4provided by RGD
Official Full Name: cholinergic receptor, muscarinic 4provided by RGD
Primary source: RGD:2344
See related: EnsemblRapid:ENSRNOG00000017556 AllianceGenome:RGD:2344
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: M4
Summary: Enables guanyl-nucleotide exchange factor activity. Predicted to be involved in G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger; adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway; and chemical synaptic transmission. Located in several cellular components, including asymmetric synapse; neuronal cell body; and sarcolemma. Is active in glutamatergic synapse; postsynaptic density membrane; and presynaptic membrane. Biomarker of congestive heart failure. Orthologous to human CHRM4 (cholinergic receptor muscarinic 4). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Brain (RPKM 24.8) and Testes (RPKM 1.9) See more
Orthologs: human mouse all
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Genomic context

Location:: 3q24

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	3	NC_086021.1 (98349080..98356821)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	3	NC_051338.1 (77893425..77901166)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	3	NC_005102.4 (80833272..80841165)

Chromosome 3 - NC_086021.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Striatal and nigral muscarinic type 1 and type 4 receptors modulate levodopa-induced dyskinesia and striato-nigral pathway activation in 6-hydroxydopamine hemilesioned rats.
Title: Striatal and nigral muscarinic type 1 and type 4 receptors modulate levodopa-induced dyskinesia and striato-nigral pathway activation in 6-hydroxydopamine hemilesioned rats.
Our data suggest that the changes in respiratory frequency and tidal volume produced by muscarinic cholinergic stimulation of the pedunculopontine tegmental nucleus is presumably mediated through a Gi-coupled M4 muscarinic receptors.
Title: M4-muscarinic acetylcholine receptor into the pedunculopontine tegmental nucleus mediates respiratory modulation of conscious rats.
Study showed that the muscarinic M4 receptor-preferring antagonist tropicamide consistently increased ERK phosphorylation in the striatum and medial prefrontal cortex.
Title: Inhibition of basal and amphetamine-stimulated extracellular signal-regulated kinase (ERK) phosphorylation in the rat forebrain by muscarinic acetylcholine M4 receptors.
M1 and M4 muscarinic receptors are involved in shock-limiting and shock-activating processes, respectively, on the course of posthemorrhagic abnormalities in systemic and hepatic portal circulation.
Title: Role of various subtypes of muscarinic cholinergic receptors in the development of posthemorrhagic abnormalities in systemic and portal circulation in rats.
Muscarinic receptor expression is developmentally regulated; M1, M3, and M4 receptors were the main subtypes expressed in oligodendrocyte progenitor cells.
Title: Muscarinic receptor subtypes as potential targets to modulate oligodendrocyte progenitor survival, proliferation, and differentiation.
M4 muscarinic acetylcholine receptors are up-regulated in dorsal root ganglion neurons and may account for increased muscarinic analgesic effects in a diabetic neuropathic pain model.
Title: Diabetic neuropathy enhances voltage-activated Ca2+ channel activity and its control by M4 muscarinic receptors in primary sensory neurons.
CaMKII binds and phosphorylates M4 upon Ca2thorn influx to augment the antagonistic action of M4 on D1 signalling and on D1-mediated, cocaine-triggered hyperlocomotion.
Title: CaMKII hunkers down on the muscarinic M4 receptor to help curb cocaine-induced hyperlocomotion.
In a Ca(2+)-sensitive manner, CaMKIIalpha regulates M4 receptor efficacy and controls the acetylcholine-dopamine balance in the basal ganglia and also the dynamics of movement.
Title: CaMKIIalpha interacts with M4 muscarinic receptors to control receptor and psychomotor function.
investigation of participation of M(4) receptors in neurotransmission at the hippocampal Schaffer collaterals-CA1 synapses; findings support permissive role of M(4) receptors & suggest their necessary participation in synaptic plasticity at these synapses
Title: M4 muscarinic receptors are involved in modulation of neurotransmission at synapses of Schaffer collaterals on CA1 hippocampal neurons in rats.
Receptor, Muscarinic M4 activated mTOR protein and its downstream molecule Ribosomal Protein S6 Kinases (70-kDa) in a 1-Phosphatidylinositol 3-Kinase-dependent manner.
Title: Regulation of mTOR and p70 S6 kinase by the muscarinic M4 receptor in PC12 cells.

Submit: New GeneRIF Correction See all GeneRIFs (19)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

UniSTS:492564 (e-PCR)
- UniSTS:492564

UniSTS:481077 (e-PCR)
- UniSTS:481077

RH134207 (e-PCR)
- UniSTS:217489

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables G protein-coupled acetylcholine receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables G protein-coupled acetylcholine receptor activity	IEA Inferred from Electronic Annotation more info
enables G protein-coupled acetylcholine receptor activity	ISO Inferred from Sequence Orthology more info
enables G protein-coupled acetylcholine receptor activity	TAS Traceable Author Statement more info	PubMed
enables guanyl-nucleotide exchange factor activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger	IBA Inferred from Biological aspect of Ancestor more info
involved_in adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in chemical synaptic transmission	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of locomotion	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in asymmetric synapse	IDA Inferred from Direct Assay more info	PubMed
located_in axon terminus	IDA Inferred from Direct Assay more info	PubMed
is_active_in dendrite	IBA Inferred from Biological aspect of Ancestor more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in postsynaptic density membrane	IDA Inferred from Direct Assay more info	PubMed
located_in postsynaptic membrane	IEA Inferred from Electronic Annotation more info
is_active_in presynaptic membrane	IDA Inferred from Direct Assay more info	PubMed
located_in sarcolemma	IDA Inferred from Direct Assay more info	PubMed
is_active_in synapse	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: muscarinic acetylcholine receptor M4

Names: cholinergic receptor, muscarin 4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_031547.1 → NP_113735.1 muscarinic acetylcholine receptor M4

See identical proteins and their annotated locations for NP_113735.1

Status: VALIDATED

Source sequence(s)

CR472483, DV213670, M16409

UniProtKB/Swiss-Prot

P08485

UniProtKB/TrEMBL

A6HNE6, G3V894

Related

ENSRNOP00000023586.4, ENSRNOT00000023583.7

Conserved Domains (1) summary

cd15298
Location:32 → 463

7tmA_mAChR_M4; muscarinic acetylcholine receptor subtype M4, member of the class A family of seven-transmembrane G protein-coupled receptors