Aspa aspartoacylase [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Aspaprovided by RGD
Official Full Name: aspartoacylaseprovided by RGD
Primary source: RGD:621693
See related: EnsemblRapid:ENSRNOG00000019659 AllianceGenome:RGD:621693
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables identical protein binding activity. Involved in central nervous system myelination and positive regulation of oligodendrocyte differentiation. Predicted to be located in cytoplasm and nucleus. Predicted to be active in cytosol. Used to study Canavan disease and essential tremor. Human ortholog(s) of this gene implicated in Canavan disease. Orthologous to human ASPA (aspartoacylase). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Kidney (RPKM 460.7), Brain (RPKM 63.0) and 5 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 10q24

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	10	NC_086028.1 (58390204..58443790, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	10	NC_051345.1 (57891704..57945267, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	10	NC_005109.4 (59839693..59888244, complement)

Chromosome 10 - NC_086028.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Myelin lesion in the aspartoacylase (Aspa) knockout rat, an animal model for Canavan disease.
Title: Myelin lesion in the aspartoacylase (Aspa) knockout rat, an animal model for Canavan disease.
Interaction of N-acetyl-L-aspartate accumulation due to ASPA deficiency with an unstable neuronal membrane potential caused by HCN1 deficiency was involved in tremor development.
Title: Involvement of aspartoacylase in tremor expression in rats.
The decrease in N-acetylaspartate following severe traumatic brain injury was characterized by profound mitochondrial malfunctioning, dramatic repression of Nat8l gene and concomitant increase in the ASPA gene and protein levels.
Title: The molecular mechanisms affecting N-acetylaspartate homeostasis following experimental graded traumatic brain injury.
This study demonistrated that aspartoacylase expression in the rat white matter central nervous system.
Title: Extensive aspartoacylase expression in the rat central nervous system.
The develomental increase of this enzyme in oligodendrocytes parallels CNS myelination.
Title: Developmental increase of aspartoacylase in oligodendrocytes parallels CNS myelination.
the N-terminal domain of aspartoacylase adopts a protein fold similar to that of zinc-dependent hydrolases related to carboxypeptidases A
Title: Structure of aspartoacylase, the brain enzyme impaired in Canavan disease.
ASPA colocalized throughout the brain with CC1, a marker for oligodendrocytes, with 92-98% of CC1-positive cells also reactive with the ASPA antibody.
Title: Immunohistochemical localization of aspartoacylase in the rat central nervous system.
describe the occurrence of ASPA within nuclei of rat brain and kidney, and in cultured rodent oligodendrocytes.
Title: Aspartoacylase is a regulated nuclear-cytoplasmic enzyme.

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General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables aspartoacylase activity	ISO Inferred from Sequence Orthology more info
enables aspartoacylase activity	ISS Inferred from Sequence or Structural Similarity more info
enables aspartoacylase activity	TAS Traceable Author Statement more info	PubMed
enables hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides	IBA Inferred from Biological aspect of Ancestor more info
enables hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides	IEA Inferred from Electronic Annotation more info
enables hydrolase activity, acting on ester bonds	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in acetate metabolic process	ISO Inferred from Sequence Orthology more info
involved_in aspartate metabolic process	ISO Inferred from Sequence Orthology more info
involved_in central nervous system myelination	IEP Inferred from Expression Pattern more info	PubMed
involved_in positive regulation of oligodendrocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: aspartoacylase

Names: ACY-2; aminoacylase-2

NP_077375.2

EC 3.5.1.15

XP_006246880.1

EC 3.5.1.15

XP_017453032.1

EC 3.5.1.15

XP_038942843.1

EC 3.5.1.15

XP_038942844.1

EC 3.5.1.15

XP_038942846.1

EC 3.5.1.15

XP_038942847.1

EC 3.5.1.15

XP_063125987.1

EC 3.5.1.15

XP_063125988.1

EC 3.5.1.15

XP_063125989.1

EC 3.5.1.15

XP_063125990.1

EC 3.5.1.15

XP_063125991.1

EC 3.5.1.15

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_024399.2 → NP_077375.2 aspartoacylase

Status: VALIDATED

Source sequence(s)

JAXUCZ010000010

UniProtKB/Swiss-Prot

Q6AZ03, Q9R1T5

UniProtKB/TrEMBL

A0A8L2RC31, A6HGJ6

Conserved Domains (1) summary

cl11393
Location:10 → 299

Peptidase_M14_like; M14 family of metallocarboxypeptidases and related proteins

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086028.1 Reference GRCr8

Range

58390204..58443790 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063269919.1 → XP_063125989.1 aspartoacylase isoform X4

UniProtKB/TrEMBL

A0A1W2Q5Z4, A0A8I6ATJ0, A6HGJ4

Related

ENSRNOP00000076074.3, ENSRNOT00000093579.3
XM_063269918.1 → XP_063125988.1 aspartoacylase isoform X4

UniProtKB/TrEMBL

A0A1W2Q5Z4, A0A8I6ATJ0, A6HGJ4
XM_039086919.2 → XP_038942847.1 aspartoacylase isoform X6

UniProtKB/TrEMBL

A0A8I6ATJ0

Conserved Domains (1) summary

cd06909
Location:11 → 192

M14_ASPA; Peptidase M14 Aspartoacylase (ASPA) subfamily
XM_063269921.1 → XP_063125991.1 aspartoacylase isoform X7

UniProtKB/TrEMBL

A0A8I6ATJ0
XM_006246818.5 → XP_006246880.1 aspartoacylase isoform X3

UniProtKB/TrEMBL

A0A1W2Q6K0, A0A8I6ATJ0

Conserved Domains (2) summary

cd06909
Location:11 → 248

M14_ASPA; Peptidase M14 Aspartoacylase (ASPA) subfamily

pfam04952
Location:9 → 247

AstE_AspA; Succinylglutamate desuccinylase / Aspartoacylase family
XM_039086915.2 → XP_038942843.1 aspartoacylase isoform X1

UniProtKB/Swiss-Prot

Q6AZ03, Q9R1T5

UniProtKB/TrEMBL

A0A8L2RC31, A6HGJ6

Related

ENSRNOP00000072876.2, ENSRNOT00000084432.4

Conserved Domains (1) summary

cl11393
Location:10 → 299

Peptidase_M14_like; M14 family of metallocarboxypeptidases and related proteins
XM_063269917.1 → XP_063125987.1 aspartoacylase isoform X2

UniProtKB/TrEMBL

A0A8L2RC31
XM_039086916.2 → XP_038942844.1 aspartoacylase isoform X1

UniProtKB/Swiss-Prot

Q6AZ03, Q9R1T5

UniProtKB/TrEMBL

A0A8L2RC31, A6HGJ6

Conserved Domains (1) summary

cl11393
Location:10 → 299

Peptidase_M14_like; M14 family of metallocarboxypeptidases and related proteins
XM_017597543.3 → XP_017453032.1 aspartoacylase isoform X2

UniProtKB/TrEMBL

A0A8L2RC31
XM_063269920.1 → XP_063125990.1 aspartoacylase isoform X5

UniProtKB/TrEMBL

A0A0H2UHY8, A0A8I6ATJ0

Related

ENSRNOP00000050760.5, ENSRNOT00000049311.6
XM_039086918.2 → XP_038942846.1 aspartoacylase isoform X5

UniProtKB/TrEMBL

A0A0H2UHY8, A0A8I6ATJ0

Conserved Domains (1) summary

cd06909
Location:11 → 205

M14_ASPA; Peptidase M14 Aspartoacylase (ASPA) subfamily