mbc myoblast city [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: mbcprovided by FlyBase
Official Full Name: myoblast cityprovided by FlyBase
Primary source: FLYBASE:FBgn0015513
Locus tag: Dmel_CG10379
See related: AllianceGenome:FB:FBgn0015513
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CED-5; CG10379; Dm MBC; Dmel\CG10379; dock180; Dock180; DOCK180; Mbc; MBC; MBC/DOCK180; Su(rac)1
Summary: Enables guanyl-nucleotide exchange factor activity. Involved in several processes, including anterior Malpighian tubule development; border follicle cell migration; and positive regulation of intracellular signal transduction. Located in cytoplasm. Is extrinsic component of cytoplasmic side of plasma membrane. Is expressed in several structures, including embryonic/larval midgut primordium; germ layer; muscle cell; myoblast; and trunk visceral muscle primordium. Human ortholog(s) of this gene implicated in immunodeficiency 40. Orthologous to several human genes including DOCK2 (dedicator of cytokinesis 2). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 95B5-95B7; 3-80 cM

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (23781765..23801634)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (19607487..19627356)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Mbc is crucial for the integrity of the F-actin foci and the FCM cytoskeleton, presumably via its activation of Rac1 in these cells.
Title: Asymmetric Mbc, active Rac1 and F-actin foci in the fusion-competent myoblasts during myoblast fusion in Drosophila.
Overexpression of MBC and ELMO in the embryonic mesoderm causes defects in myoblast fusion reminiscent of those seen with constitutively-activated Rac1
Title: Drosophila ELMO/CED-12 interacts with Myoblast city to direct myoblast fusion and ommatidial organization.
MBC can function in myoblast fusion through a pathway that may involve phosphatidylinositol 3,4,5-triphosphate binding but is independent of direct adapter protein Crk binding.
Title: The CDM superfamily protein MBC directs myoblast fusion through a mechanism that requires phosphatidylinositol 3,4,5-triphosphate binding but is independent of direct interaction with DCrk.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables guanyl-nucleotide exchange factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables guanyl-nucleotide exchange factor activity	IEA Inferred from Electronic Annotation more info
enables guanyl-nucleotide exchange factor activity	IGI Inferred from Genetic Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables small GTPase binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in anterior Malpighian tubule development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in border follicle cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell competition in a multicellular organism	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell migration	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell morphogenesis	TAS Traceable Author Statement more info	PubMed
involved_in central nervous system development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dorsal closure	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in imaginal disc fusion, thorax closure	IGI Inferred from Genetic Interaction more info	PubMed
involved_in larval visceral muscle development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in muscle attachment	IGI Inferred from Genetic Interaction more info	PubMed
involved_in myoblast fusion	IBA Inferred from Biological aspect of Ancestor more info
involved_in myoblast fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of JNK cascade	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of Rac protein signal transduction	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of border follicle cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in small GTPase-mediated signal transduction	IEA Inferred from Electronic Annotation more info
involved_in vascular endothelial growth factor receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in extrinsic component of cytoplasmic side of plasma membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in ruffle	TAS Traceable Author Statement more info	PubMed

General protein information

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Preferred Names: myoblast city

Names: CG10379-PA; CG10379-PB; mbc-PA; mbc-PB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

23781765..23801634

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001260338.2 → NP_001247267.1 myoblast city, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001247267.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4K7Q6, V5NZW8

Conserved Domains (4) summary

cd08694
Location:443 → 633

C2_Dock-A; C2 domains found in Dedicator Of CytoKinesis (Dock) class A proteins

cd11697
Location:1296 → 1697

DHR2_DOCK_A; Dock Homology Region 2, a GEF domain, of Class A Dedicator of Cytokinesis proteins

cd11872
Location:14 → 70

SH3_DOCK_AB; Src Homology 3 domain of Class A and B Dedicator of Cytokinesis proteins

pfam16172
Location:86 → 435

DOCK_N; DOCK N-terminus
NM_057796.4 → NP_477144.1 myoblast city, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_477144.1

Status: REVIEWED

UniProtKB/TrEMBL

O16103, Q9VCH4, V5NZW8

Related

FBpp0083850

Conserved Domains (4) summary

cd08694
Location:443 → 633

C2_Dock-A; C2 domains found in Dedicator Of CytoKinesis (Dock) class A proteins

cd11697
Location:1258 → 1659

DHR2_DOCK_A; Dock Homology Region 2, a GEF domain, of Class A Dedicator of Cytokinesis proteins

cd11872
Location:14 → 70

SH3_DOCK_AB; Src Homology 3 domain of Class A and B Dedicator of Cytokinesis proteins

pfam16172
Location:86 → 435

DOCK_N; DOCK N-terminus