pk prickle [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: pkprovided by FlyBase
Official Full Name: prickleprovided by FlyBase
Primary source: FLYBASE:FBgn0003090
Locus tag: Dmel_CG11084
See related: AllianceGenome:FB:FBgn0003090
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG11084; CG12830; Dmel\CG11084; Pk; PK; pk-sple; Pk-Sple; pkM; sple; Sple
Summary: Predicted to enable zinc ion binding activity. Involved in several processes, including establishment of planar polarity; establishment or maintenance of microtubule cytoskeleton polarity; and positive regulation of cell projection organization. Located in plasma membrane. Is expressed in several structures, including appendage; embryonic/larval epidermis; embryonic/larval optic lobe; epithelial furrow; and imaginal disc. Used to study epilepsy. Human ortholog(s) of this gene implicated in progressive myoclonus epilepsy 1B. Orthologous to several human genes including PRICKLE2 (prickle planar cell polarity protein 2). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 42F3-43A1; 2-55 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (7150703..7223378)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (3038208..3110883)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Prickle isoform participation in distinct polarization events in the Drosophila eye.
Title: Prickle isoform participation in distinct polarization events in the Drosophila eye.
Prickle isoforms determine handedness of helical morphogenesis.
Title: Prickle isoforms determine handedness of helical morphogenesis.
removal of Dishevelled in one cell causes rapid release of Prickle into the cytoplasm in the neighboring cell.
Title: Rapid Disruption of Dishevelled Activity Uncovers an Intercellular Role in Maintenance of Prickle in Core Planar Polarity Protein Complexes.
the prickle gene and its protein products Prickle and Spiny leg can affect, separately, both Dachsous/Fat, and the 'core' or Starry night/Frizzled systems; however, neither Prickle nor Spiny leg are essential components of the Dachsous/Fat or the Starry night/Frizzled system, nor do they act as a functional link between the two systems.
Title: Planar cell polarity: the prickle gene acts independently on both the Ds/Ft and the Stan/Fz systems.
The stabilizing function of Prickle on Frizzled requires Prickle activity in neighboring cells. Conversely, Prickle in the same cell has a destabilizing effect on Frizzled.
Title: A Dual Function for Prickle in Regulating Frizzled Stability during Feedback-Dependent Amplification of Planar Polarity.
Data indicate Prickle protein as a mitogen-activated protein kinase Nemo substrate.
Title: Prickle is phosphorylated by Nemo and targeted for degradation to maintain Prickle/Spiny-legs isoform balance during planar cell polarity establishment.
We find that the E3 ubiquitin ligase complex Cullin1(Cul1)/SkpA/Supernumerary limbs(Slimb) regulates the stability of one of the peripheral membrane components, Prickle (Pk).
Title: Clustering and negative feedback by endocytosis in planar cell polarity signaling is modulated by ubiquitinylation of prickle.
Pk and Sple form different complexes with distinct subcellular localizations in the eye and wing.
Title: Dachsous-dependent asymmetric localization of spiny-legs determines planar cell polarity orientation in Drosophila.
Fat and Core planar cell polarity (PCP) pathways patterns change during morphogenesis, undergoing phases of coupling and uncoupling that are regulated by antagonistic Core PCP protein isoforms Prickle and Spiny-legs.
Title: The balance of prickle/spiny-legs isoforms controls the amount of coupling between core and fat PCP systems.
These data reveal a previously unidentified pathway in the pathophysiology of seizure disorders and provide evidence for a more generalized cellular mechanism whereby Prickle mediates polarity by influencing microtubule-mediated transport.
Title: prickle modulates microtubule polarity and axonal transport to ameliorate seizures in flies.

Submit: New GeneRIF Correction See all GeneRIFs (22)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

mobile_element

Loc: 7209778-7209843 mobile_element_type = transposon:INE-1{}3030

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in anterior/posterior axis specification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in axonal transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of imaginal disc-derived wing hair orientation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of ommatidial planar polarity	IGI Inferred from Genetic Interaction more info	PubMed
involved_in establishment of ommatidial planar polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of planar polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of protein localization	TAS Traceable Author Statement more info	PubMed
involved_in establishment of tissue polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment or maintenance of cell polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment or maintenance of microtubule cytoskeleton polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in maintenance of protein location	TAS Traceable Author Statement more info	PubMed
involved_in morphogenesis of a polarized epithelium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ommatidial rotation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of axon extension	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	TAS Traceable Author Statement more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: prickle

Names: CG11084-PA; CG11084-PB; CG11084-PC; pk-PA; pk-PB; pk-PC; prickle-spiny legs; prickle-spiny-legs; spiny leg; spiny legs

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

7150703..7223378

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_165508.2 → NP_724534.1 prickle, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_724534.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A1Z6W3

Related

FBpp0088113

Conserved Domains (4) summary

cd09415
Location:288 → 346

LIM1_Prickle; The first LIM domain of Prickle

cd09418
Location:351 → 406

LIM2_Prickle; The second LIM domain of Prickle

cd09420
Location:411 → 469

LIM3_Prickle; The third LIM domain of Prickle

cd09827
Location:187 → 282

PET_Prickle; The PET domain of Prickle
NM_165509.2 → NP_724535.1 prickle, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_724535.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A1Z6W3

Related

FBpp0088114

Conserved Domains (4) summary

cd09415
Location:354 → 412

LIM1_Prickle; The first LIM domain of Prickle

cd09418
Location:417 → 472

LIM2_Prickle; The second LIM domain of Prickle

cd09420
Location:477 → 535

LIM3_Prickle; The third LIM domain of Prickle

cd09827
Location:253 → 348

PET_Prickle; The PET domain of Prickle
NM_165512.2 → NP_724538.1 prickle, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_724538.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A1Z6V4, A1Z6V8, A1Z6W3, Q9N9H6, Q9U5X0, Q9U5X1

UniProtKB/TrEMBL

F3YDG0

Related

FBpp0088115

Conserved Domains (4) summary

cd09415
Location:624 → 682

LIM1_Prickle; The first LIM domain of Prickle

cd09418
Location:687 → 742

LIM2_Prickle; The second LIM domain of Prickle

cd09420
Location:747 → 805

LIM3_Prickle; The third LIM domain of Prickle

cd09827
Location:523 → 618

PET_Prickle; The PET domain of Prickle