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    Ssu72 Ssu72 CTD phosphatase [ Drosophila melanogaster (fruit fly) ]

    Gene ID: 32973, updated on 9-Dec-2024

    Summary

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    Official Symbol
    Ssu72provided by FlyBase
    Official Full Name
    Ssu72 CTD phosphataseprovided by FlyBase
    Primary source
    FLYBASE:FBgn0031054
    Locus tag
    Dmel_CG14216
    See related
    AllianceGenome:FB:FBgn0031054
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Drosophila melanogaster
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
    Also known as
    BEST:CK01830; CG14216; Dmel\CG14216; dSsu72
    Summary
    Enables RNA polymerase II CTD heptapeptide repeat phosphatase activity. Predicted to be involved in mRNA 3'-end processing and termination of RNA polymerase II transcription. Predicted to be located in nucleus. Predicted to be part of mRNA cleavage and polyadenylation specificity factor complex. Is expressed in organism. Orthologous to several human genes including SSU72 (SSU72 homolog, RNA polymerase II CTD phosphatase); SSU72L1 (SSU72 like 1); and SSU72L2 (SSU72 like 2). [provided by Alliance of Genome Resources, Dec 2024]
    Orthologs
    all
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    Genomic context

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    See Ssu72 in Genome Data Viewer
    Location:
    18D13-18D13; 1-63 cM
    Exon count:
    3
    Annotation release Status Assembly Chr Location
    Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) X NC_004354.4 (19664561..19666866)
    Release 5.57 previous assembly Release 5 (GCF_000001215.2) X NC_004354.3 (19558594..19560899)

    Chromosome X - NC_004354.4Genomic Context describing neighboring genes Neighboring gene elys Neighboring gene uncharacterized protein Neighboring gene antisense RNA:CR44512 Neighboring gene Zwischenferment Neighboring gene eye transformer

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. cis-Proline-mediated Ser(P)5 dephosphorylation by the RNA polymerase II C-terminal domain phosphatase Ssu72. Werner-Allen JW, et al. J Biol Chem, 2011 Feb 18. PMID 21159777, Free PMC Article
    2. ¹H, ¹³C and ¹⁵N backbone and side-chain resonance assignments of Drosophila melanogaster Ssu72. Werner-Allen JW, et al. Biomol NMR Assign, 2012 Apr. PMID 21732054, Free PMC Article
    3. Crystal structure of Ssu72, an essential eukaryotic phosphatase specific for the C-terminal domain of RNA polymerase II, in complex with a transition state analogue. Zhang Y, et al. Biochem J, 2011 Mar 15. PMID 21204787
    4. novel modifications on C-terminal domain of RNA polymerase II can fine-tune the phosphatase activity of Ssu72. Luo Y, et al. ACS Chem Biol, 2013 Sep 20. PMID 23844594, Free PMC Article
    5. Structural determinants for accurate dephosphorylation of RNA polymerase II by its cognate C-terminal domain (CTD) phosphatase during eukaryotic transcription. Irani S, et al. J Biol Chem, 2019 May 24. PMID 30971428, Free PMC Article

    See all (38) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Ssu72 dephosphorylates pSer(5) effectively but only has low activities toward pSer(7) and pSer(2) The structural analysis revealed that Ssu72 requires that the proline residue in the substrate's SP motif is in the cis configuration, forming a tight beta-turn for recognition by Ssu72.
      Title: Structural determinants for accurate dephosphorylation of RNA polymerase II by its cognate C-terminal domain (CTD) phosphatase during eukaryotic transcription.
    2. Determined are the kinetic and structural properties of Drosophila Ssu72-symplekin in complex with the C-terminal domain of RNA polymerase II peptide with consecutive phosphorylated Thr4 and Ser5. [Ssu72]
      Title: novel modifications on C-terminal domain of RNA polymerase II can fine-tune the phosphatase activity of Ssu72.
    3. The Ssu72 structure exhibits a core fold with a similar topology to that of LMWPTPs [low-molecular-mass PTPs (protein tyrosine phosphatases)].
      Title: Crystal structure of Ssu72, an essential eukaryotic phosphatase specific for the C-terminal domain of RNA polymerase II, in complex with a transition state analogue.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    mobile_element

    • Loc: 19666231-19666846 mobile_element_type = transposon:G5A{}5651

    Gene Ontology Provided by FlyBase

    Function Evidence Code Pubs
    enables RNA polymerase II CTD heptapeptide repeat phosphatase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables RNA polymerase II CTD heptapeptide repeat phosphatase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables phosphatase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables phosphoprotein phosphatase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in mRNA 3'-end processing ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in mRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in termination of RNA polymerase II transcription IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    part_of mRNA cleavage and polyadenylation specificity factor complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleus ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  

    General protein information

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    Preferred Names
    Ssu72 CTD phosphatase
    Names
    CG14216-PA
    CG14216-PB
    CG14216-PC
    Ssu72 ortholog
    Ssu72-PA
    Ssu72-PB
    Ssu72-PC
    NP_001259725.1
    NP_001285455.1
    NP_608342.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_004354.4 Reference assembly

      Range
      19664561..19666866
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_134498.4 → NP_608342.1  Ssu72 CTD phosphatase, isoform A [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_608342.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q9VWE4
      Related
      FBpp0074516
      Conserved Domains (1) summary
      pfam04722
      Location:7 → 195
      Ssu72; Ssu72-like protein

    2. NM_001272796.2 → NP_001259725.1  Ssu72 CTD phosphatase, isoform B [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001259725.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      M9PHZ3
      Conserved Domains (1) summary
      pfam04722
      Location:7 → 194
      Ssu72; Ssu72-like protein

    3. NM_001298526.1 → NP_001285455.1  Ssu72 CTD phosphatase, isoform C [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001285455.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q9VWE4
      Conserved Domains (1) summary
      pfam04722
      Location:7 → 195
      Ssu72; Ssu72-like protein
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    M9PHZ3 M9PHZ3
    Q9VWE4 Q9VWE4

    Gene LinkOut

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