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    Vps8 Vacuolar protein sorting 8 [ Drosophila melanogaster (fruit fly) ]

    Gene ID: 38735, updated on 9-Dec-2024

    Summary

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    Official Symbol
    Vps8provided by FlyBase
    Official Full Name
    Vacuolar protein sorting 8provided by FlyBase
    Primary source
    FLYBASE:FBgn0035704
    Locus tag
    Dmel_CG10144
    See related
    AllianceGenome:FB:FBgn0035704
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Drosophila melanogaster
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
    Also known as
    anon-WO0118547.365; CG10144; Dmel\CG10144; vps8
    Summary
    Predicted to enable GTPase binding activity and zinc ion binding activity. Involved in endocytosis; endosomal vesicle fusion; and endosome organization. Located in early endosome. Part of CORVET complex. Is expressed in adult head; embryonic/larval garland cell; and pericardial cell. Orthologous to human VPS8 (VPS8 subunit of CORVET complex). [provided by Alliance of Genome Resources, Dec 2024]
    Orthologs
    all
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    Genomic context

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    See Vps8 in Genome Data Viewer
    Location:
    65B3-65B3; 3-17 cM
    Exon count:
    4
    Annotation release Status Assembly Chr Location
    Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 3L NT_037436.4 (6491230..6495297, complement)
    Release 5.57 previous assembly Release 5 (GCF_000001215.2) 3L NT_037436.3 (6484330..6488397, complement)

    Chromosome 3L - NT_037436.4Genomic Context describing neighboring genes Neighboring gene uncharacterized protein Neighboring gene bulli Neighboring gene sulfateless Neighboring gene long non-coding RNA:CR45979 Neighboring gene beta-Tubulin at 65B

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. A trimeric metazoan Rab7 GEF complex is crucial for endocytosis and scavenger function. Dehnen L, et al. J Cell Sci, 2020 Jul 9. PMID 32499409
    2. Intracellular vesicle trafficking plays an essential role in mitochondrial quality control. Gerards M, et al. Mol Biol Cell, 2018 Apr 1. PMID 29343549, Free PMC Article
    3. Comparative genomic analysis of multi-subunit tethering complexes demonstrates an ancient pan-eukaryotic complement and sculpting in Apicomplexa. Klinger CM, et al. PLoS One, 2013. PMID 24086721, Free PMC Article
    4. Tethering complexes in the endocytic pathway: CORVET and HOPS. Solinger JA, et al. FEBS J, 2013 Jun. PMID 23351085
    5. MiniCORVET is a Vps8-containing early endosomal tether in Drosophila. Lőrincz P, et al. Elife, 2016 Jun 2. PMID 27253064, Free PMC Article

    See all (39) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Here, the authors show that Vps8 overexpression inhibits homotypic fusion and vacuole protein sorting (HOPS)-dependent trafficking routes including late endosome maturation, autophagosome-lysosome fusion, crinophagy and lysosome-related organelle formation. Mechanistically, Vps8 overexpression abolishes the late endosomal localization of HOPS-specific Vps41/Lt and prevents HOPS assembly.
      Title: Vps8 overexpression inhibits HOPS-dependent trafficking routes by outcompeting Vps41/Lt.
    2. Study shows that Drosophila Vps8 is highly expressed in hemocytes and nephrocytes, and localizes to early endosomes despite the lack of a clear Vps3 homolog; Vps8 forms a complex and acts together with Vps16A, Dor/Vps18 and Car/Vps33A, and loss of any of these proteins leads to fragmentation of endosomes.
      Title: MiniCORVET is a Vps8-containing early endosomal tether in Drosophila.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by FlyBase

    Function Evidence Code Pubs
    enables GTPase binding ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables zinc ion binding ISM
    Inferred from Sequence Model
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in endocytosis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in endosomal transport ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    involved_in endosomal vesicle fusion IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in endosomal vesicle fusion IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in endosomal vesicle fusion IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in endosome organization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein targeting to vacuole IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein targeting to vacuole IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in vesicle-mediated transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of CORVET complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of CORVET complex ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    part_of HOPS complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in early endosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in late endosome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    vacuolar protein sorting 8
    Names
    CG10144-PA
    Vps8-PA

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NT_037436.4 Reference assembly

      Range
      6491230..6495297 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_139791.3 → NP_648048.1  vacuolar protein sorting 8 [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_648048.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      B7FNP6, C5WLL9, D0IQG0, Q9VRX2
      Related
      FBpp0076695
      Conserved Domains (4) summary
      sd00039
      Location:123 → 156
      7WD40; WD40 repeat [structural motif]
      pfam00637
      Location:905 → 1008
      Clathrin; Region in Clathrin and VPS
      pfam12816
      Location:554 → 725
      Vps8; Golgi CORVET complex core vacuolar protein 8
      cl17238
      Location:1146 → 1189
      RING_Ubox; The superfamily of RING finger (Really Interesting New Gene) domain and U-box domain
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9VRX2.1 Q9VRX2

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Other Literature Sources
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