msl-2 male-specific lethal 2 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: msl-2provided by FlyBase
Official Full Name: male-specific lethal 2provided by FlyBase
Primary source: FLYBASE:FBgn0005616
Locus tag: Dmel_CG3241
See related: AllianceGenome:FB:FBgn0005616
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG3241; Dmel\CG3241; km(2)A; kmA; msl; MSL; Msl 1-3; Msl-2; MSL-2; msl2; Msl2; MSL2
Summary: Enables several functions, including histone H2B ubiquitin ligase activity; molecular condensate scaffold activity; and nucleic acid binding activity. Involved in dosage compensation by hyperactivation of X chromosome; protein localization to chromatin; and protein polyubiquitination. Located in X chromosome and nuclear chromosome. Part of MSL complex and X chromosome located dosage compensation complex, transcription activating. Is expressed in several structures, including adult midgut epithelial cell; adult posterior midgut; ganglia; and somatic precursor cell. Orthologous to human MSL2 (MSL complex subunit 2). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 23F3-23F3; 2-10 cM

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (3462218..3466112, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (3462218..3466112, complement)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Functional Role of C-terminal Domains in the MSL2 Protein of Drosophila melanogaster.
Title: Functional Role of C-terminal Domains in the MSL2 Protein of Drosophila melanogaster.
Physical interaction between MSL2 and CLAMP assures direct cooperativity and prevents competition at composite binding sites.
Title: Physical interaction between MSL2 and CLAMP assures direct cooperativity and prevents competition at composite binding sites.
Structural basis for interaction between CLAMP and MSL2 proteins involved in the specific recruitment of the dosage compensation complex in Drosophila.
Title: Structural basis for interaction between CLAMP and MSL2 proteins involved in the specific recruitment of the dosage compensation complex in Drosophila.
RNA nucleation by MSL2 induces selective X chromosome compartmentalization.
Title: RNA nucleation by MSL2 induces selective X chromosome compartmentalization.
proper MSL2 positioning requires an interaction with either CLAMP or DNA to initiate dosage compensation in Drosophila males.
Title: The simultaneous interaction of MSL2 with CLAMP and DNA provides redundancy in the initiation of dosage compensation in Drosophila males.
hese are explained by physical interaction between MSL2 and CLAMP. In vivo, both factors cooperate to compete with nucleosome formation at HAS. The male-specific MSL2 thus synergises with a ubiquitous GA-repeat binding protein for refined X/autosome discrimination
Title: Factor cooperation for chromosome discrimination in Drosophila.
The data is consistent with a model where Hrp48 inhibits msl-2 translation by targeting eIF3d.
Title: Hrp48 and eIF3d contribute to msl-2 mRNA translational repression.
the only male-specific protein within the Male-Specific Lethal complex; in addition to regulating the X chromosome, targets autosomal genes involved in patterning and morphogenesis
Title: Facultative dosage compensation of developmental genes on autosomes in Drosophila and mouse embryonic stem cells.
HOW is required for SXL to retain msl2 transcripts in the nucleus
Title: Sex-lethal promotes nuclear retention of msl2 mRNA via interactions with the STAR protein HOW.
Solution structure of MSL2 CXC domain reveals an unusual Zn3Cys9 cluster and similarity to pre-SET domains of histone lysine methyltransferases.
Title: Solution structure of MSL2 CXC domain reveals an unusual Zn3Cys9 cluster and similarity to pre-SET domains of histone lysine methyltransferases.

Submit: New GeneRIF Correction See all GeneRIFs (26)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

mobile_element

Loc: c3463066-3462712 mobile_element_type = transposon:INE-1{}4974

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables chromatin-protein adaptor activity	IDA Inferred from Direct Assay more info	PubMed
enables histone H2B ubiquitin ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables lncRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables molecular condensate scaffold activity	IDA Inferred from Direct Assay more info	PubMed
enables protein-RNA adaptor activity	IDA Inferred from Direct Assay more info	PubMed
enables sequence-specific double-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin protein ligase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in dosage compensation by hyperactivation of X chromosome	IDA Inferred from Direct Assay more info	PubMed
involved_in dosage compensation by hyperactivation of X chromosome	NAS Non-traceable Author Statement more info	PubMed
involved_in protein localization to chromatin	IDA Inferred from Direct Assay more info	PubMed
involved_in protein polyubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in sex-chromosome dosage compensation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of MSL complex	IBA Inferred from Biological aspect of Ancestor more info
part_of MSL complex	IDA Inferred from Direct Assay more info	PubMed
part_of MSL complex	IEA Inferred from Electronic Annotation more info
part_of MSL complex	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in X chromosome	IDA Inferred from Direct Assay more info	PubMed
located_in X chromosome	IDA Inferred from Direct Assay more info	PubMed
part_of X chromosome located dosage compensation complex, transcription activating	IDA Inferred from Direct Assay more info	PubMed
part_of X chromosome located dosage compensation complex, transcription activating	NAS Non-traceable Author Statement more info	PubMed
located_in chromosome	IMP Inferred from Mutant Phenotype more info	PubMed
part_of dosage compensation complex	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear chromosome	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: male-specific lethal 2

Names: CG3241-PA; CG3241-PB; Male-Specific lethal 2; killer of males; killer-of-males-A; male specifc lethal; male specific lethal 2; male specific-lethal 2; male-specific-lethal 2; male-specific-lethal-2; males specific lethal 2; msl-2-PA; msl-2-PB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

3462218..3466112 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_164536.2 → NP_722907.1 male-specific lethal 2, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_722907.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P50534, Q9VQR1

UniProtKB/TrEMBL

A4V038, A8B9Z4

Related

FBpp0077259

Conserved Domains (2) summary

pfam16682
Location:520 → 567

MSL2-CXC; CXC domain of E3 ubiquitin-protein ligase MSL2

pfam16685
Location:39 → 111

zf-RING_10; zinc RING finger of MSL2
NM_078743.5 → NP_523467.1 male-specific lethal 2, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_523467.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P50534, Q9VQR1

UniProtKB/TrEMBL

A4V038, A8B9Z4

Related

FBpp0077260

Conserved Domains (2) summary

pfam16682
Location:520 → 567

MSL2-CXC; CXC domain of E3 ubiquitin-protein ligase MSL2

pfam16685
Location:39 → 111

zf-RING_10; zinc RING finger of MSL2