Tnfaip3 tumor necrosis factor, alpha-induced protein 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Tnfaip3provided by MGI
Official Full Name: tumor necrosis factor, alpha-induced protein 3provided by MGI
Primary source: MGI:MGI:1196377
See related: Ensembl:ENSMUSG00000019850 AllianceGenome:MGI:1196377
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: A20; Tnfip3
Summary: Enables kinase binding activity. Involved in several processes, including negative regulation of cytokine production; negative regulation of defense response; and negative regulation of signal transduction. Acts upstream of or within establishment of protein localization to vacuole; positive regulation of protein catabolic process; and protein K63-linked deubiquitination. Located in cytoplasm. Is expressed in central nervous system; genitourinary system; heart; and retina. Human ortholog(s) of this gene implicated in breast cancer; colorectal cancer; esophageal cancer; and familial Behcet-like autoinflammatory syndrome. Orthologous to human TNFAIP3 (TNF alpha induced protein 3). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Broad expression in thymus adult (RPKM 11.6), spleen adult (RPKM 5.3) and 20 other tissues See more
Orthologs: human all
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Genomic context

Location:: 10 A3; 10 8.08 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	10	NC_000076.7 (18876658..18891158, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	10	NC_000076.6 (19000910..19015410, complement)

Chromosome 10 - NC_000076.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Myeloid A20 is critical for alternative macrophage polarization and type-2 immune-mediated helminth resistance.
Title: Myeloid A20 is critical for alternative macrophage polarization and type-2 immune-mediated helminth resistance.
Degradation of Ubiquitin-Editing Enzyme A20 following Autophagy Activation Promotes RNF168 Nuclear Translocation and NF-kappaB Activation in Lupus Nephritis.
Title: Degradation of Ubiquitin-Editing Enzyme A20 following Autophagy Activation Promotes RNF168 Nuclear Translocation and NF-κB Activation in Lupus Nephritis.
A20 regulates lymphocyte adhesion in murine neuroinflammation by restricting endothelial ICOSL expression in the CNS.
Title: A20 regulates lymphocyte adhesion in murine neuroinflammation by restricting endothelial ICOSL expression in the CNS.
Protective effect of TNFAIP3 on testosterone production in Leydig cells under an aging inflammatory microenvironment.
Title: Protective effect of TNFAIP3 on testosterone production in Leydig cells under an aging inflammatory microenvironment.
METTL14-Mediated m6A Modification of TNFAIP3 Involved in Inflammation in Patients With Active Rheumatoid Arthritis.
Title: METTL14-Mediated m6A Modification of TNFAIP3 Involved in Inflammation in Patients With Active Rheumatoid Arthritis.
Knockdown of A20 attenuates microglial susceptibility to OGD/R-induced ferroptosis and upregulates inflammatory responses.
Title: Knockdown of A20 attenuates microglial susceptibility to OGD/R-induced ferroptosis and upregulates inflammatory responses.
A20 is a master switch of IL-33 signaling in macrophages and determines IL-33-induced lung immunity.
Title: A20 is a master switch of IL-33 signaling in macrophages and determines IL-33-induced lung immunity.
The impact of the cytoplasmic ubiquitin ligase TNFAIP3 gene variation on transcription factor NF-kappaB activation in acute kidney injury.
Title: The impact of the cytoplasmic ubiquitin ligase TNFAIP3 gene variation on transcription factor NF-κB activation in acute kidney injury.
A20 and the noncanonical NF-kappaB pathway are key regulators of neutrophil recruitment during fetal ontogeny.
Title: A20 and the noncanonical NF-κB pathway are key regulators of neutrophil recruitment during fetal ontogeny.
CircRalgapa1 facilitates morphine tolerance via miR-873a-5p/A20 axis in mice.
Title: CircRalgapa1 facilitates morphine tolerance via miR-873a-5p/A20 axis in mice.

Submit: New GeneRIF Correction See all GeneRIFs (158)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (1) 1 citation
Endonuclease-mediated (13) 1 citation
Targeted (17) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables K63-linked deubiquitinase activity	ISO Inferred from Sequence Orthology more info
enables K63-linked polyubiquitin modification-dependent protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables cysteine-type deubiquitinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables cysteine-type deubiquitinase activity	ISO Inferred from Sequence Orthology more info
enables cysteine-type deubiquitinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables kinase binding	IDA Inferred from Direct Assay more info	PubMed
enables protease binding	IEA Inferred from Electronic Annotation more info
enables protease binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activity	ISS Inferred from Sequence or Structural Similarity more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in B-1 B cell homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in cell migration	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to hydrogen peroxide	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to lipopolysaccharide	ISO Inferred from Sequence Orthology more info
involved_in cellular response to lipopolysaccharide	ISS Inferred from Sequence or Structural Similarity more info
involved_in cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within establishment of protein localization to vacuole	IDA Inferred from Direct Assay more info	PubMed
involved_in inflammatory response	IEA Inferred from Electronic Annotation more info
involved_in marginal zone B cell differentiation	NAS Non-traceable Author Statement more info	PubMed
involved_in negative regulation of B cell activation	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of CD40 signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of CD40 signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of NF-kappaB transcription factor activity	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of autophagy	NAS Non-traceable Author Statement more info	PubMed
involved_in negative regulation of canonical NF-kappaB signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of canonical NF-kappaB signal transduction	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of chronic inflammatory response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cyclin-dependent protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of endothelial cell apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of endothelial cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of extrinsic apoptotic signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of extrinsic apoptotic signaling pathway via death domain receptors	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of extrinsic apoptotic signaling pathway via death domain receptors	NAS Non-traceable Author Statement more info	PubMed
involved_in negative regulation of granuloma formation	NAS Non-traceable Author Statement more info	PubMed
involved_in negative regulation of heterotypic cell-cell adhesion	NAS Non-traceable Author Statement more info	PubMed
involved_in negative regulation of inflammatory response	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of inflammatory response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of innate immune response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of interleukin-1 beta production	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of interleukin-1 beta production	ISO Inferred from Sequence Orthology more info	PubMed
involved_in negative regulation of interleukin-2 production	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of interleukin-2 production	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of interleukin-6 production	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of nucleotide-binding oligomerization domain containing 1 signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of protein ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of smooth muscle cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of smooth muscle cell proliferation	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of toll-like receptor 3 signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of toll-like receptor 3 signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of toll-like receptor 5 signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of tumor necrosis factor production	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of Wnt signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of hepatocyte proliferation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein catabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein K11-linked deubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein K11-linked deubiquitination	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein K48-linked deubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein K48-linked deubiquitination	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein K48-linked ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein K48-linked ubiquitination	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within protein K63-linked deubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein K63-linked deubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein K63-linked deubiquitination	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein deubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein deubiquitination involved in ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteolysis	IEA Inferred from Electronic Annotation more info
involved_in regulation of germinal center formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of immunoglobulin production	IC Inferred by Curator more info	PubMed
involved_in regulation of innate immune response	IC Inferred by Curator more info	PubMed
involved_in response to molecule of bacterial origin	IDA Inferred from Direct Assay more info	PubMed
involved_in response to molecule of bacterial origin	ISO Inferred from Sequence Orthology more info
involved_in response to muramyl dipeptide	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to wounding	NAS Non-traceable Author Statement more info	PubMed
involved_in tolerance induction to lipopolysaccharide	IEA Inferred from Electronic Annotation more info
involved_in tolerance induction to lipopolysaccharide	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in lysosome	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: tumor necrosis factor alpha-induced protein 3

Names: TNF alpha-induced protein 3; putative DNA-binding protein A20; tumor necrosis factor induced protein 3; zinc finger protein A20

NP_001159874.1

EC 3.4.19.12

NP_033423.3

EC 3.4.19.12

XP_006512765.1

EC 3.4.19.12

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001166402.1 → NP_001159874.1 tumor necrosis factor alpha-induced protein 3 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) contains a distinct 5' UTR and 5' coding region, compared to variant 1. The resulting protein (isoform 2) has a longer N-terminus, compared to isoform 1.

Source sequence(s)

AC102657, AK171326, BC054806

UniProtKB/TrEMBL

Q3U695, Q7TQD1

Related

ENSMUSP00000101167.2, ENSMUST00000105527.2

Conserved Domains (2) summary

smart00259
Location:781 → 806

ZnF_A20; A20-like zinc fingers

pfam02338
Location:135 → 294

OTU; OTU-like cysteine protease
NM_001428864.1 → NP_001415793.1 tumor necrosis factor alpha-induced protein 3 isoform 1

Status: VALIDATED

Source sequence(s)

AC102657

UniProtKB/Swiss-Prot

Q3U968, Q60769

UniProtKB/TrEMBL

Q3U695, Q7TQD1
NM_001428865.1 → NP_001415794.1 tumor necrosis factor alpha-induced protein 3 isoform 3

Status: VALIDATED

Source sequence(s)

AC102657

UniProtKB/TrEMBL

Q3U695, Q7TQD1
NM_001428866.1 → NP_001415795.1 tumor necrosis factor alpha-induced protein 3 isoform 4

Status: VALIDATED

Source sequence(s)

AC102657

UniProtKB/TrEMBL

Q3U695
NM_009397.4 → NP_033423.3 tumor necrosis factor alpha-induced protein 3 isoform 1

See identical proteins and their annotated locations for NP_033423.3

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes isoform 1. Variants 1 and 3 both encode the same isoform (1).

Source sequence(s)

AC102657

Consensus CDS

CCDS23715.1

UniProtKB/Swiss-Prot

Q3U968, Q60769

UniProtKB/TrEMBL

Q3U695, Q7TQD1

Related

ENSMUSP00000019997.5, ENSMUST00000019997.11

Conserved Domains (3) summary

smart00259
Location:744 → 769

ZnF_A20; A20-like zinc fingers

pfam02338
Location:98 → 257

OTU; OTU-like cysteine protease

pfam15548
Location:528 → 622

DUF4655; Domain of unknown function (DUF4655)

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000076.7 Reference GRCm39 C57BL/6J

Range

18876658..18891158 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006512702.4 → XP_006512765.1 tumor necrosis factor alpha-induced protein 3 isoform X1

Conserved Domains (3) summary

smart00259
Location:744 → 769

ZnF_A20; A20-like zinc fingers

pfam02338
Location:98 → 257

OTU; OTU-like cysteine protease

pfam15548
Location:528 → 622

DUF4655; Domain of unknown function (DUF4655)