cup cup [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: cupprovided by FlyBase
Official Full Name: cupprovided by FlyBase
Primary source: FLYBASE:FBgn0000392
Locus tag: Dmel_CG11181
See related: AllianceGenome:FB:FBgn0000392
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG11181; Cup; CUP; DmCup; Dmel\CG11181; fs(1)cup; fs(2)cup; i239
Summary: Enables RNA binding activity; eukaryotic initiation factor 4E binding activity; and translation regulator activity. Involved in several processes, including meiotic nuclear division; negative regulation of eukaryotic translation initiation factor 4F complex assembly; and negative regulation of translation. Located in several cellular components, including cytoplasmic ribonucleoprotein granule; neuromuscular junction; and terminal bouton. Colocalizes with endoplasmic reticulum exit site. Is expressed in several structures, including adult brain; adult brain cell body rind; adult head; germline cell; and gonad. Orthologous to human EIF4ENIF1 (eukaryotic translation initiation factor 4E nuclear import factor 1). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 26F6-26F7; 2-21 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (6663664..6674780)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (6663664..6674780)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cup is essential for oskar mRNA translational repression during early Drosophila oogenesis.
Title: Cup is essential for oskar mRNA translational repression during early Drosophila oogenesis.
loss of cup disrupts the localization of both the oskar mRNA and its associated proteins to the posterior pole of the developing oocyte.
Title: Cup regulates oskar mRNA stability during oogenesis.
cup mutants have increased numbers of satellite boutons and EPSP. cup genetically interacts with EndoA and Dap160.
Title: The translational regulator Cup controls NMJ presynaptic terminal morphology.
Cup promotes the accumulation of the germ plasm determinants at the posterior pole of the oocyte.
Title: The translational repressor Cup is required for germ cell development in Drosophila.
The structure provides insight into the mechanism by which Cup disrupts eIF4E-eIF4G interaction and has broader implications for understanding the role of 4E-BPs in translational regulation.
Title: Crystal structure of a minimal eIF4E-Cup complex reveals a general mechanism of eIF4E regulation in translational repression.
Cup blocks the precocious activation of the orb autoregulatory loop
Title: Cup blocks the precocious activation of the orb autoregulatory loop.
n addition to inducing translational repression, CUP maintains mRNA targets in a repressed state by promoting their deadenylation and protects deadenylated mRNAs from further degradation
Title: CUP promotes deadenylation and inhibits decapping of mRNA targets.
This review describes Cup as a multifunctional protein directly and/or indirectly affecting the activity of different mRNAs and proteins by diverse molecular mechanisms in order to achieve a broad range of biological functions.
Title: A cup full of functions.
Hsp90 interacts in vitro with the translational repressor Cup.
Title: The molecular chaperone Hsp90 is a component of the cap-binding complex and interacts with the translational repressor Cup during Drosophila oogenesis.
Transcription of comets and cups occurs just before protamines can be detected in spermatid nuclei.
Title: Comet and cup genes in Drosophila spermatogenesis: the first demonstration of post-meiotic transcription.

Submit: New GeneRIF Correction See all GeneRIFs (17)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables eukaryotic initiation factor 4E binding	IDA Inferred from Direct Assay more info	PubMed
enables mRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables translation regulator activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in dorsal/ventral pattern formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in female meiotic nuclear division	IGI Inferred from Genetic Interaction more info	PubMed
involved_in follicle cell of egg chamber migration	HMP more info	PubMed
involved_in intracellular mRNA localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic chromosome segregation	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of eukaryotic translation initiation factor 4F complex assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of oskar mRNA translation	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of oskar mRNA translation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of translation	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of translation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of translation	IPI Inferred from Physical Interaction more info	PubMed
involved_in negative regulation of translational initiation	IDA Inferred from Direct Assay more info	PubMed
involved_in oocyte growth in germarium-derived egg chamber	IGI Inferred from Genetic Interaction more info	PubMed
involved_in oogenesis	HMP more info	PubMed
involved_in oogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of pole plasm oskar mRNA localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of synaptic assembly at neuromuscular junction	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
NOT located_in P-body	IDA Inferred from Direct Assay more info	PubMed
located_in P-body	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	HDA more info	PubMed
colocalizes_with endoplasmic reticulum exit site	IDA Inferred from Direct Assay more info	PubMed
located_in neuromuscular junction	IDA Inferred from Direct Assay more info	PubMed
located_in neuronal ribonucleoprotein granule	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in terminal bouton	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: cup

Names: CG11181-PB; CG11181-PC; Cup; cup-PB; cup-PC

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

6663664..6674780

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001298758.1 → NP_001285687.1 cup, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001285687.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O02432, Q9VMA3

UniProtKB/TrEMBL

X2J9P9

Conserved Domains (1) summary

pfam10631
Location:227 → 305

DUF2477; Protein of unknown function (DUF2477)
NM_078769.3 → NP_523493.3 cup, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_523493.3

Status: REVIEWED

UniProtKB/Swiss-Prot

O02432, Q9VMA3

UniProtKB/TrEMBL

X2J9P9

Conserved Domains (1) summary

pfam10631
Location:227 → 305

DUF2477; Protein of unknown function (DUF2477)