Flot2 flotillin 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Flot2provided by RGD
Official Full Name: flotillin 2provided by RGD
Primary source: RGD:70993
See related: EnsemblRapid:ENSRNOG00000009681 AllianceGenome:RGD:70993
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables ionotropic glutamate receptor binding activity and protease binding activity. Predicted to be involved in several processes, including negative regulation of macromolecule metabolic process; positive regulation of canonical NF-kappaB signal transduction; and protein localization to cell periphery. Located in caveola. Part of flotillin complex. Is active in glutamatergic synapse and presynaptic active zone membrane. Orthologous to human FLOT2 (flotillin 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Heart (RPKM 188.6), Adrenal (RPKM 173.7) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 10q25

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	10	NC_086028.1 (63424084..63445811)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	10	NC_051345.1 (62926047..62947759)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	10	NC_005109.4 (65304901..65329332, complement)

Chromosome 10 - NC_086028.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

the presence of Flotillin-1, in addition to Flotillin-2 in the cardiac intercalated disc, is reported.
Title: Flotillins in the intercalated disc are potential modulators of cardiac excitability.
Data suggest that flotillin-1 and flotillin-2 function as thyroglobulin-binding proteins in lipid rafts of thyrocytes; these interactions appear to exert negative-feedback effects on gene expression in thyrocytes.
Title: A Novel Role for Flotillin-Containing Lipid Rafts in Negative-Feedback Regulation of Thyroid-Specific Gene Expression by Thyroglobulin.
upregulation of reggie-1 in mammalian neurons provides nerve cells with neuron-intrinsic properties required for axon growth and successful regeneration
Title: Upregulation of reggie-1/flotillin-2 promotes axon regeneration in the rat optic nerve in vivo and neurite growth in vitro.
Flot-2 expression decreases after spinal cord injury.
Title: Expression profile of flotillin-2 and its pathophysiological role after spinal cord injury.
flotillins have a role in NPC1L1-mediated cholesterol uptake and NPC1L1-flotillins-postive cholesterol-enriched membrane microdomains are involved in the mechanism for efficient cholesterol absorption
Title: Flotillins play an essential role in Niemann-Pick C1-like 1-mediated cholesterol uptake.
Endocytosis of flotillins is regulated by the Src family kinase Fyn.
Title: Endocytosis of flotillin-1 and flotillin-2 is regulated by Fyn kinase.
NMDARs were significantly more colocalized with flot-1 than flot-2 in cultured hippocampal neurons
Title: NMDA receptors interact with flotillin-1 and -2, lipid raft-associated proteins.
deletion mutants of reggie-1/flotillin-2 accumulate in the Golgi complex, suggesting Golgi-dependent trafficking of reggie-1/flotillin-2
Title: Trafficking of the microdomain scaffolding protein reggie-1/flotillin-2.
Reggie-1 and reggie-2 are localized in microdomains different from caveolae, and the function of reggies is different from and independent of caveolin-1.
Title: Reggie-1 and reggie-2 localize in non-caveolar rafts in epithelial cells: cellular localization is not dependent on the expression of caveolin proteins.
Myristoylation and palmitoylation of flotillin-2 are necessary for raft membrane association.
Title: Membrane and raft association of reggie-1/flotillin-2: role of myristoylation, palmitoylation and oligomerization and induction of filopodia by overexpression.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI548110 (e-PCR)
- UniSTS:243480

RH138487 (e-PCR)
- UniSTS:220574

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ionotropic glutamate receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables protease binding	IBA Inferred from Biological aspect of Ancestor more info
enables protease binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in anterograde dendritic transport	IEA Inferred from Electronic Annotation more info
involved_in anterograde dendritic transport	ISO Inferred from Sequence Orthology more info
involved_in cell adhesion	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of amyloid precursor protein catabolic process	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of amyloid precursor protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of gene expression	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of canonical NF-kappaB signal transduction	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of establishment of T cell polarity	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of establishment of T cell polarity	ISO Inferred from Sequence Orthology more info
involved_in protein localization to plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein localization to plasma membrane	IEA Inferred from Electronic Annotation more info
involved_in protein localization to plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in protein localization to plasma membrane raft	IEA Inferred from Electronic Annotation more info
involved_in protein localization to plasma membrane raft	ISO Inferred from Sequence Orthology more info
involved_in protein stabilization	IEA Inferred from Electronic Annotation more info
involved_in protein stabilization	ISO Inferred from Sequence Orthology more info
NOT involved_in regulation of heterotypic cell-cell adhesion	ISO Inferred from Sequence Orthology more info
involved_in regulation of myoblast differentiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of myoblast differentiation	IEA Inferred from Electronic Annotation more info
involved_in regulation of myoblast differentiation	ISO Inferred from Sequence Orthology more info
involved_in regulation of postsynaptic membrane neurotransmitter receptor levels	IEA Inferred from Electronic Annotation more info
involved_in regulation of postsynaptic membrane neurotransmitter receptor levels	ISO Inferred from Sequence Orthology more info
NOT involved_in regulation of toll-like receptor 3 signaling pathway	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in acrosomal membrane	IEA Inferred from Electronic Annotation more info
located_in acrosomal membrane	ISO Inferred from Sequence Orthology more info
located_in adherens junction	IEA Inferred from Electronic Annotation more info
located_in adherens junction	ISO Inferred from Sequence Orthology more info
NOT located_in apical plasma membrane	ISO Inferred from Sequence Orthology more info
located_in basolateral plasma membrane	IEA Inferred from Electronic Annotation more info
located_in basolateral plasma membrane	ISO Inferred from Sequence Orthology more info
located_in caveola	IDA Inferred from Direct Assay more info	PubMed
located_in caveola	ISO Inferred from Sequence Orthology more info
located_in cell-cell contact zone	IEA Inferred from Electronic Annotation more info
located_in cell-cell contact zone	ISO Inferred from Sequence Orthology more info
located_in cortical actin cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cortical actin cytoskeleton	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasmic vesicle	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasmic vesicle	ISO Inferred from Sequence Orthology more info
located_in dendrite cytoplasm	IEA Inferred from Electronic Annotation more info
located_in endocytic vesicle	IEA Inferred from Electronic Annotation more info
located_in endocytic vesicle	ISO Inferred from Sequence Orthology more info
located_in endocytic vesicle	ISS Inferred from Sequence or Structural Similarity more info
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in endosome	ISO Inferred from Sequence Orthology more info
located_in endosome	ISS Inferred from Sequence or Structural Similarity more info
part_of flotillin complex	IBA Inferred from Biological aspect of Ancestor more info
part_of flotillin complex	IDA Inferred from Direct Assay more info	PubMed
part_of flotillin complex	IEA Inferred from Electronic Annotation more info
part_of flotillin complex	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	EXP Inferred from Experiment more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in glutamatergic synapse	IEA Inferred from Electronic Annotation more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in lamellipodium	IEA Inferred from Electronic Annotation more info
located_in lamellipodium	ISO Inferred from Sequence Orthology more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in membrane raft	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in presynaptic active zone membrane	EXP Inferred from Experiment more info	PubMed
is_active_in presynaptic active zone membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in synapse	EXP Inferred from Experiment more info	PubMed
is_active_in synapse	IDA Inferred from Direct Assay more info	PubMed
located_in uropod	IEA Inferred from Electronic Annotation more info
located_in uropod	ISO Inferred from Sequence Orthology more info
located_in vesicle	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: flotillin-2

Names: REG-1; reggie-1; reggie1-1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001270800.1 → NP_001257729.1 flotillin-2 isoform 2

See identical proteins and their annotated locations for NP_001257729.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) has an alternate coding exon of the same size in place of an internal coding exon compared to variant 1. The resulting isoform (2) is the same size as isoform 1 but has a different internal segment compared to isoform 1.

Source sequence(s)

BC083550, CB581049

UniProtKB/Swiss-Prot

Q9QX33, Q9Z2S8, Q9Z2S9

UniProtKB/TrEMBL

A6HH01, A6HH02

Conserved Domains (2) summary

COG2268
Location:6 → 417

YqiK; Uncharacterized membrane protein YqiK, contains Band7/PHB/SPFH domain [Function unknown]

cd03399
Location:34 → 178

SPFH_flotillin; Flotillin or reggie family; SPFH (stomatin, prohibitin, flotillin, and HflK/C) superfamily
NM_001270801.1 → NP_001257730.1 flotillin-2 isoform 3

See identical proteins and their annotated locations for NP_001257730.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) has an alternate coding exon in place of an internal coding exon compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus compared to isoform 1.

Source sequence(s)

BC083550, CB581049, JAXUCZ010000010

UniProtKB/TrEMBL

A0A0G2JXA8, A6HH02, A6HH03

Related

ENSRNOP00000070199.1, ENSRNOT00000078757.3

Conserved Domains (2) summary

COG2268
Location:1 → 368

YqiK; Uncharacterized membrane protein YqiK, contains Band7/PHB/SPFH domain [Function unknown]

cd03399
Location:1 → 129

SPFH_flotillin; Flotillin or reggie family; SPFH (stomatin, prohibitin, flotillin, and HflK/C) superfamily
NM_031830.2 → NP_114018.1 flotillin-2 isoform 1

See identical proteins and their annotated locations for NP_114018.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1).

Source sequence(s)

BC083550, CB581049, JAXUCZ010000010

UniProtKB/TrEMBL

A6HH00, A6HH02

Related

ENSRNOP00000014118.5, ENSRNOT00000014118.9

Conserved Domains (2) summary

COG2268
Location:6 → 417

YqiK; Uncharacterized membrane protein YqiK, contains Band7/PHB/SPFH domain [Function unknown]

cd03399
Location:34 → 178

SPFH_flotillin; Flotillin or reggie family; SPFH (stomatin, prohibitin, flotillin, and HflK/C) superfamily

RNA

NR_073076.1 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (4) lacks an alternate coding exon compared to variant 1. This variant is represented as non-coding due to the presence of an upstream ORF that is predicted to interfere with translation of the longest ORF; translation of the upstream ORF renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

BC083550, CB581049, JAXUCZ010000010
NR_073077.1 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (5) lacks an alternate coding exon compared to variant 1. This variant is represented as non-coding due to the presence of an upstream ORF that is predicted to interfere with translation of the longest ORF; translation of the upstream ORF renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

BC083550, CB581049

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086028.1 Reference GRCr8

Range

63424084..63445811

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063269972.1 → XP_063126042.1 flotillin-2 isoform X1

UniProtKB/TrEMBL

A0A0G2JXA8, A6HH03

Related

ENSRNOP00000014104.7, ENSRNOT00000014104.9
XM_017597552.3 → XP_017453041.1 flotillin-2 isoform X2

UniProtKB/TrEMBL

A6HH02, Q5XIW9

Conserved Domains (2) summary

COG2268
Location:8 → 340

YqiK; Uncharacterized membrane protein YqiK, contains Band7/PHB/SPFH domain [Function unknown]

cd03399
Location:1 → 101

SPFH_flotillin; Flotillin or reggie family; SPFH (stomatin, prohibitin, flotillin, and HflK/C) superfamily