spas spastin [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: spasprovided by FlyBase
Official Full Name: spastinprovided by FlyBase
Primary source: FLYBASE:FBgn0039141
Locus tag: Dmel_CG5977
See related: AllianceGenome:FB:FBgn0039141
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG5977; D-spastin; Dmel\CG5977; Dspastin; Spas; SPAS; SPAST; Spastin
Summary: Enables microtubule binding activity and microtubule severing ATPase activity. Involved in several processes, including regulation of microtubule depolymerization; regulation of nervous system development; and regulation of neuromuscular synaptic transmission. Located in several cellular components, including centrosome; neuromuscular junction; and terminal bouton. Colocalizes with synaptic vesicle. Is expressed in several structures, including anterior midgut primordium; cephalic furrow; embryonic neuromere; embryonic/larval head sense organ; and ganglia. Used to study hereditary spastic paraplegia and hereditary spastic paraplegia 4. Human ortholog(s) of this gene implicated in hereditary spastic paraplegia 4. Orthologous to human SPAST (spastin). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 95D9-95D9; 3-82 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (24038215..24042982)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (19863937..19868704)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A ~3.2 A resolution cryo-EM structure of the D. melanogaster spastin hexamer with a polyglutamate peptide bound in its central pore reveals how concurrent nucleotide and substrate binding organizes the conserved spastin pore loops into an ordered network that is allosterically coupled to oligomerization. 2 electropositive loops arranged in a double-helical staircase coordinate the substrate sidechains.
Title: An allosteric network in spastin couples multiple activities required for microtubule severing.
targeting sequence directs spastin to the surface of lipid droplets. Depletion of the homologous spastin proteins in both flies and worms affects lipid droplet number and triacylglycerol content.
Title: Spastin binds to lipid droplets and affects lipid metabolism.
analysis of spastin's microtubule-binding properties and comparison with katanin
Title: Spastin's microtubule-binding properties and comparison to katanin.
The spastin is a key regulator of axon regeneration, and initiation of regeneration is extremely sensitive to the spastin copy number.
Title: Normal spastin gene dosage is specifically required for axon regeneration.
Dendritic growth is regulated by Kruppel-like factor dendritic arbor reduction protein 1 (Dar1) suppressing the expression of microtubule-severing protein spastin.
Title: Differential regulation of dendritic and axonal development by the novel Krüppel-like factor Dar1.
spastin is essential for apical domain biogenesis during rhabdomere elongation in Drosophila photoreceptor morphogenesis
Title: Role of spastin in apical domain control along the rhabdomere elongation in Drosophila photoreceptor.
Exogenous expression of wild-type Drosophila or human spastin rescues behavioral and cellular defects in spastin null flies equivalently.
Title: Functional conservation of human Spastin in a Drosophila model of autosomal dominant-hereditary spastic paraplegia.
X ray structure; data support a model in which spastin pulls the C terminus of tubulin through its central pore, generating a mechanical force that destabilizes tubulin-tubulin interactions within the microtubule lattice
Title: Structural basis of microtubule severing by the hereditary spastic paraplegia protein spastin.
Spastin and Fidgetin are utilized to stimulate microtubule minus-end depolymerization and flux; they concentrate at centrosomes, where they catalyze the turnover of gamma-tubulin.
Title: Three microtubule severing enzymes contribute to the "Pacman-flux" machinery that moves chromosomes.
D-spastin displays ATPase activity and uses energy from ATP hydrolysis to sever and disassemble microtubules; disease mutations abolish or partially interfere with these activities.
Title: The Drosophila homologue of the hereditary spastic paraplegia protein, spastin, severs and disassembles microtubules.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Dm1592 (e-PCR)
- UniSTS:5038

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables alpha-tubulin binding	NAS Non-traceable Author Statement more info	PubMed
enables microtubule binding	IDA Inferred from Direct Assay more info	PubMed
enables microtubule binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables microtubule severing ATPase activity	IDA Inferred from Direct Assay more info	PubMed
enables microtubule severing ATPase activity	IEA Inferred from Electronic Annotation more info
enables microtubule severing ATPase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in hemocyte migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in locomotory behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule severing	IDA Inferred from Direct Assay more info	PubMed
involved_in microtubule severing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic sister chromatid segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of microtubule depolymerization	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of neuromuscular synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of synaptic assembly at neuromuscular junction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of axon extension involved in regeneration	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of dendrite morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of lipid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of microtubule depolymerization	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of microtubule depolymerization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of neuromuscular synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of synaptic assembly at neuromuscular junction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of terminal button organization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in microtubule cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in microtubule cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
located_in neuromuscular junction	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with synaptic vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in terminal bouton	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: spastin

Names: CG5977-PA; CG5977-PB; CG5977-PC; CG5977-PD; CG5977-PE; CG5977-PF; D-spastin; Dm-Spastin; dspastin; spas-PA; spas-PB; spas-PC; spas-PD; spas-PE; spas-PF; spasin

NP_001262896.1

EC 5.6.1.1

NP_001287494.1

EC 5.6.1.1

NP_001303437.1

EC 5.6.1.1

NP_001303438.1

EC 5.6.1.1

NP_651206.3

EC 5.6.1.1

NP_732941.2

EC 5.6.1.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

24038215..24042982

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001300565.1 → NP_001287494.1 spastin, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001287494.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4LHJ5

Conserved Domains (5) summary

smart00382
Location:453 → 590

AAA; ATPases associated with a variety of cellular activities

cd02679
Location:230 → 308

MIT_spastin; MIT: domain contained within Microtubule Interacting and Trafficking molecules. This MIT domain sub-family is found in the AAA protein spastin, a probable ATPase involved in the assembly or function of nuclear protein complexes; spastins might also be ...

pfam00004
Location:457 → 588

AAA; ATPase family associated with various cellular activities (AAA)

pfam09336
Location:660 → 692

Vps4_C; Vps4 C terminal oligomerization domain

cl21455
Location:420 → 476

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases
NM_170115.3 → NP_732941.2 spastin, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_732941.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q8I0P1, Q8IMX5, Q8T066

Related

FBpp0083918

Conserved Domains (5) summary

smart00382
Location:515 → 652

AAA; ATPases associated with a variety of cellular activities

cd02679
Location:230 → 308

MIT_spastin; MIT: domain contained within Microtubule Interacting and Trafficking molecules. This MIT domain sub-family is found in the AAA protein spastin, a probable ATPase involved in the assembly or function of nuclear protein complexes; spastins might also be ...

pfam00004
Location:519 → 650

AAA; ATPase family associated with various cellular activities (AAA)

pfam09336
Location:722 → 754

Vps4_C; Vps4 C terminal oligomerization domain

cl21455
Location:482 → 538

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases
NM_001275967.2 → NP_001262896.1 spastin, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001262896.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4LHJ5

Conserved Domains (5) summary

smart00382
Location:453 → 590

AAA; ATPases associated with a variety of cellular activities

cd02679
Location:230 → 308

MIT_spastin; MIT: domain contained within Microtubule Interacting and Trafficking molecules. This MIT domain sub-family is found in the AAA protein spastin, a probable ATPase involved in the assembly or function of nuclear protein complexes; spastins might also be ...

pfam00004
Location:457 → 588

AAA; ATPase family associated with various cellular activities (AAA)

pfam09336
Location:660 → 692

Vps4_C; Vps4 C terminal oligomerization domain

cl21455
Location:420 → 476

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases
NM_001316508.1 → NP_001303437.1 spastin, isoform F [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

A0A126GV13

Conserved Domains (5) summary

smart00382
Location:246 → 383

AAA; ATPases associated with a variety of cellular activities

cd02679
Location:23 → 101

MIT_spastin; MIT: domain contained within Microtubule Interacting and Trafficking molecules. This MIT domain sub-family is found in the AAA protein spastin, a probable ATPase involved in the assembly or function of nuclear protein complexes; spastins might also be ...

pfam00004
Location:250 → 381

AAA; ATPase family associated with various cellular activities (AAA)

pfam09336
Location:453 → 485

Vps4_C; Vps4 C terminal oligomerization domain

cl21455
Location:213 → 269

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases
NM_001316509.1 → NP_001303438.1 spastin, isoform E [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

A0A126GV10

Conserved Domains (5) summary

smart00382
Location:308 → 445

AAA; ATPases associated with a variety of cellular activities

cd02679
Location:23 → 101

MIT_spastin; MIT: domain contained within Microtubule Interacting and Trafficking molecules. This MIT domain sub-family is found in the AAA protein spastin, a probable ATPase involved in the assembly or function of nuclear protein complexes; spastins might also be ...

pfam00004
Location:312 → 443

AAA; ATPase family associated with various cellular activities (AAA)

pfam09336
Location:515 → 547

Vps4_C; Vps4 C terminal oligomerization domain

cl21455
Location:275 → 331

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases
NM_142949.4 → NP_651206.3 spastin, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_651206.3

Status: REVIEWED

UniProtKB/Swiss-Prot

Q8I0P1, Q8IMX5, Q8T066

Related

FBpp0083919

Conserved Domains (5) summary

smart00382
Location:515 → 652

AAA; ATPases associated with a variety of cellular activities

cd02679
Location:230 → 308

MIT_spastin; MIT: domain contained within Microtubule Interacting and Trafficking molecules. This MIT domain sub-family is found in the AAA protein spastin, a probable ATPase involved in the assembly or function of nuclear protein complexes; spastins might also be ...

pfam00004
Location:519 → 650

AAA; ATPase family associated with various cellular activities (AAA)

pfam09336
Location:722 → 754

Vps4_C; Vps4 C terminal oligomerization domain

cl21455
Location:482 → 538

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases