Rok Rho kinase [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Rokprovided by FlyBase
Official Full Name: Rho kinaseprovided by FlyBase
Primary source: FLYBASE:FBgn0026181
Locus tag: Dmel_CG9774
See related: AllianceGenome:FB:FBgn0026181
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG9774; Dmel\CG9774; DRhk; drok; dRok; dROK; Drok; DRok; DROK; MRLC; Rhk; RhoK; rock; Rock; ROCk; ROCK; ROCK-1; ROCK1; ROCK2; rok; ROK; Rok/ROCK; ROKalpha/beta
Summary: Enables GTPase binding activity and protein serine/threonine kinase activity. Involved in several processes, including cytoskeleton organization; maintenance of epithelial cell apical/basal polarity; and morphogenesis of an epithelium. Located in apicomedial cortex and cell-cell contact zone. Colocalizes with apical cortex. Is expressed in imaginal disc and organism. Orthologous to human ROCK1 (Rho associated coiled-coil containing protein kinase 1) and ROCK2 (Rho associated coiled-coil containing protein kinase 2). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 14F2-14F4; 1-54 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	X	NC_004354.4 (16624012..16637414, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	X	NC_004354.3 (16518045..16531447, complement)

Chromosome X - NC_004354.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

polarity-induced spatiotemporal control of Rok and Pkn is important for unequal cortical expansion, ensuring correct cleavage furrow positioning and the establishment of physical asymmetry.
Title: Cell Polarity Regulates Biased Myosin Activity and Dynamics during Asymmetric Cell Division via Drosophila Rho Kinase and Protein Kinase N.
Loss of DRok function in tendon cells results in mis-orientation of tendon cell extensions and abnormal accumulation of Thrombospondin and betaPS-integrin, which may cause abnormal myotendinous junction formation and muscle morphogenesis.
Title: Establishment of the Muscle-Tendon Junction During Thorax Morphogenesis in Drosophila Requires the Rho-Kinase.
model in which Rok-induced phosphorylation of residues within the basic region mediates the activation of FHOD1 homolog Knittrig in controlling macrophage migration
Title: The Drosophila FHOD1-like formin Knittrig acts through Rok to promote stress fiber formation and directed macrophage migration during the cellular immune response.
Atonal and EGFR signalling orchestrate rok- and Drak-dependent adherens junction remodelling during ommatidia morphogenesis.
Title: Atonal and EGFR signalling orchestrate rok- and Drak-dependent adherens junction remodelling during ommatidia morphogenesis.
The structure of Shrm domain 2 (SD2), which mediates the interaction with Rock and is required for Shrm function, is reported.
Title: Structure of Shroom domain 2 reveals a three-segmented coiled-coil required for dimerization, Rock binding, and apical constriction.
Data show that that Drak broadly promotes proper morphogenesis of epithelial tissues during development, and Drak activity is largely redundant with that of the Drosophila ROCK orthologue, Rok.
Title: Overlapping roles of Drosophila Drak and Rok kinases in epithelial tissue morphogenesis.
In parallel to regulation of crb RNA and protein, Rho1 activity also signals through Rho-kinase (Rok) to induce apical constriction and cell shape change during invagination of salivary gland.
Title: Rho GTPase controls invagination and cohesive migration of the Drosophila salivary gland through Crumbs and Rho-kinase.
activation of RhoA/ROCK and PI3K/Akt plays a pivotal role in leptin signaling, leading to the development of VSMC hypertrophy through a mechanism involving altered actin dynamics
Title: Actin cytoskeleton dynamics promotes leptin-induced vascular smooth muscle hypertrophy via RhoA/ROCK- and phosphatidylinositol 3-kinase/protein kinase B-dependent pathways.
Drok and Drosophila Lim-kinase interact in the developing nervous system
Title: Rho-kinase regulates tissue morphogenesis via non-muscle myosin and LIM-kinase during Drosophila development.
CD44 interaction with p115RhoGEF and ROK plays a pivotal role in promoting Gab-1 phosphorylation leading to Gab-1.PI 3-kinase membrane localization, AKT signaling, and cytokine (M-CSF) production during HA-mediated breast cancer progression
Title: Hyaluronan-mediated CD44 interaction with RhoGEF and Rho kinase promotes Grb2-associated binder-1 phosphorylation and phosphatidylinositol 3-kinase signaling leading to cytokine (macrophage-colony stimulating factor) production and breast tumor progression.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Dm0519 (e-PCR)
- UniSTS:39074

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables GTPase binding	IPI Inferred from Physical Interaction more info	PubMed
enables Rho-dependent protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables small GTPase binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in Rho protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in Rho protein signal transduction	IPI Inferred from Physical Interaction more info	PubMed
involved_in actin cytoskeleton organization	IEA Inferred from Electronic Annotation more info
involved_in actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actomyosin structure organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in apical constriction	TAS Traceable Author Statement more info	PubMed
involved_in apical constriction involved in gastrulation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in assembly of apicomedial cortex actomyosin	IDA Inferred from Direct Assay more info	PubMed
involved_in assembly of apicomedial cortex actomyosin	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cortical actin cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in dorsal closure	TAS Traceable Author Statement more info	PubMed
involved_in embryonic morphogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in epidermis development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of imaginal disc-derived wing hair orientation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of ommatidial planar polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of planar polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of planar polarity	TAS Traceable Author Statement more info	PubMed
involved_in establishment of protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in female germline ring canal stabilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in imaginal disc-derived wing hair organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in imaginal disc-derived wing hair organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in maintenance of epithelial cell apical/basal polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in maintenance of protein location in cell cortex	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic cytokinesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic cytokinesis	IDA Inferred from Direct Assay more info	PubMed
involved_in mitotic cytokinesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic cytokinesis, division site positioning	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle elongation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in morphogenesis of embryonic epithelium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear axial expansion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nurse cell nucleus anchoring	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oocyte axis specification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oocyte development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ovarian nurse cell to oocyte transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of stress fiber assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of wound healing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in regulation of actin cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of actin filament bundle assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of actin filament bundle assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of axonogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of cell junction assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of cell junction assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of cell shape	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of myoblast fusion	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
colocalizes_with apical cortex	IDA Inferred from Direct Assay more info	PubMed
located_in apicomedial cortex	IDA Inferred from Direct Assay more info	PubMed
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in cell-cell contact zone	IDA Inferred from Direct Assay more info	PubMed
located_in cell-cell junction	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytosol	TAS Traceable Author Statement more info	PubMed

General protein information

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Preferred Names: Rho kinase

Names: CG9774-PA; CG9774-PB; CG9774-PC; CG9774-PD; DRho-kinase; Rho-associated kinase; Rho-associated protein kinase; Rho-kinase; RhoA kinase; Rok-PA; Rok-PB; Rok-PC; Rok-PD; drosophila rho-associated kinase; rho kinase; rock

NP_001285335.1

EC 2.7.11.1

NP_001285336.1

EC 2.7.11.1

NP_001285337.1

EC 2.7.11.1

NP_536796.2

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_004354.4 Reference assembly

Range

16624012..16637414 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001298407.1 → NP_001285336.1 Rho kinase, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001285336.1

Status: REVIEWED

UniProtKB/TrEMBL

Q961D4, Q9U779, X2JE40

Conserved Domains (6) summary

smart00109
Location:1254 → 1307

C1; Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains)

smart00220
Location:87 → 349

S_TKc; Serine/Threonine protein kinases, catalytic domain

COG0419
Location:484 → 1088

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

cd01242
Location:1134 → 1250

PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain

cd05596
Location:61 → 411

STKc_ROCK; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase

pfam08912
Location:963 → 1027

Rho_Binding; Rho Binding
NM_001298406.1 → NP_001285335.1 Rho kinase, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001285335.1

Status: REVIEWED

UniProtKB/TrEMBL

Q961D4, Q9U779, X2JC83

Conserved Domains (6) summary

smart00109
Location:1245 → 1298

C1; Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains)

smart00220
Location:87 → 349

S_TKc; Serine/Threonine protein kinases, catalytic domain

COG0419
Location:484 → 1088

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

cd01242
Location:1125 → 1241

PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain

cd05596
Location:61 → 411

STKc_ROCK; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase

pfam08912
Location:963 → 1027

Rho_Binding; Rho Binding
NM_080535.3 → NP_536796.2 Rho kinase, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_536796.2

Status: REVIEWED

UniProtKB/TrEMBL

Q961D4, Q9U779, Q9VXE3

Related

FBpp0074061

Conserved Domains (6) summary

smart00109
Location:1254 → 1307

C1; Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains)

smart00220
Location:87 → 349

S_TKc; Serine/Threonine protein kinases, catalytic domain

COG0419
Location:484 → 1088

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

cd01242
Location:1134 → 1250

PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain

cd05596
Location:61 → 411

STKc_ROCK; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase

pfam08912
Location:963 → 1027

Rho_Binding; Rho Binding
NM_001298408.1 → NP_001285337.1 Rho kinase, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001285337.1

Status: REVIEWED

UniProtKB/TrEMBL

Q961D4, Q9U779, X2JKQ8

Conserved Domains (6) summary

smart00109
Location:1245 → 1298

C1; Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains)

smart00220
Location:78 → 340

S_TKc; Serine/Threonine protein kinases, catalytic domain

COG0419
Location:475 → 1079

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

cd01242
Location:1125 → 1241

PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain

cd05596
Location:52 → 402

STKc_ROCK; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase

pfam08912
Location:954 → 1018

Rho_Binding; Rho Binding