Prdm14 PR domain containing 14 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Prdm14provided by MGI
Official Full Name: PR domain containing 14provided by MGI
Primary source: MGI:MGI:3588194
See related: Ensembl:ENSMUSG00000042414 AllianceGenome:MGI:3588194
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Summary: Enables histone methyltransferase binding activity; methyltransferase regulator activity; and nucleic acid binding activity. Involved in germ cell development; positive regulation of flagellated sperm motility; and positive regulation of stem cell population maintenance. Acts upstream of or within several processes, including embryo implantation; inner cell mass cell fate commitment; and negative regulation of macromolecule biosynthetic process. Located in nucleus. Is expressed in several structures, including hemolymphoid system; intestine; liver; lung; and yolk sac. Human ortholog(s) of this gene implicated in lung non-small cell carcinoma. Orthologous to human PRDM14 (PR/SET domain 14). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in placenta adult (RPKM 1.1), colon adult (RPKM 0.1) and 1 other tissue See more
Orthologs: human all
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Genomic context

Location:: 1 A3; 1 4.1 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (13183649..13197467, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (13113428..13127163, complement)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Interplay between CTCF boundaries and a super enhancer controls cohesin extrusion trajectories and gene expression.
Title: Interplay between CTCF boundaries and a super enhancer controls cohesin extrusion trajectories and gene expression.
Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats.
Title: Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats.
the crucial role of Prdm14 in primordial germ cell specification is conserved between rat and mice
Title: Germline development in rat revealed by visualization and deletion of Prdm14.
Authors find that global upregulation of the repressive histone H3 lysine 27 trimethylation (H3K27me3) mark is PRDM14 dosage dependent in PGCs of both sexes. When focusing on XCR, we observed that PRDM14 is required for removal of H3K27me3 from the inactive X-chromosome, which, in contrast to global upregulation, takes place progressively along the PGC migration path.
Title: PRDM14 controls X-chromosomal and global epigenetic reprogramming of H3K27me3 in migrating mouse primordial germ cells.
The pluripotency regulator PRDM14 requires the master hematopoietic regulator CBFA2T3 to initiate leukemia in progenitor cells, demonstrating an oncogenic role for CBFA2T3 and providing an avenue for targeting cancer-initiating cells.
Title: The Pluripotency Regulator PRDM14 Requires Hematopoietic Regulator CBFA2T3 to Initiate Leukemia in Mice.
the co-option of the PRDM14-CBFA2T complex from motor neurons into pluripotent cells may have maintained the transcriptional network for pluripotency during vertebrate evolution.
Title: Co-option of the PRDM14-CBFA2T complex from motor neurons to pluripotent cells during vertebrate evolution.
we provide evidence that HOXA1 displays an unexpectedly long half-life and demonstrate that PRDM14 can reduce the stability and affect the transcriptional activity of HOXA1.
Title: PRDM14, a putative histone methyl-transferase, interacts with and decreases the stability and activity of the HOXA1 transcription factor.
Our results suggest that PRDM14 serves as an initial trigger for epigenetic changes and hyperdynamic plasticity in tumors via bivalent chromatin domains.
Title: Silencing PRDM14 expression by an innovative RNAi therapy inhibits stemness, tumorigenicity, and metastasis of breast cancer.
PRDM14 recruited OCT3/4 to the enhancer regions of naive pluripotency genes via TET-base excision repair-mediated demethylation.
Title: PRDM14 Drives OCT3/4 Recruitment via Active Demethylation in the Transition from Primed to Naive Pluripotency.
JARID2 physically interacts with ESRRB, SALL4A, and PRDM14
Title: Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells.

Submit: New GeneRIF Correction See all GeneRIFs (25)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (5) 1 citation
Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Prdm14 (e-PCR), detects polymorphism
- UniSTS:547297

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	IC Inferred by Curator more info
enables RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables chromatin DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables histone methyltransferase binding	IPI Inferred from Physical Interaction more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables methyltransferase activity	IEA Inferred from Electronic Annotation more info
enables methyltransferase regulator activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within cell fate specification	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cell morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within embryo implantation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within epigenetic regulation of gene expression	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within fertilization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of fibroblast growth factor receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within germ cell development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germ cell development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within germ-line stem cell population maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within homeostasis of number of cells within a tissue	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within inactivation of paternal X chromosome by genomic imprinting	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within inner cell mass cell fate commitment	IDA Inferred from Direct Assay more info	PubMed
involved_in methylation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within negative regulation of fibroblast growth factor receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of gene expression, epigenetic	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of flagellated sperm motility	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of stem cell population maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within stem cell population maintenance	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: PR domain zinc finger protein 14

Names: PR domain-containing protein 14

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001081209.3 → NP_001074678.2 PR domain zinc finger protein 14

See identical proteins and their annotated locations for NP_001074678.2

Status: VALIDATED

Source sequence(s)

AC091248

Consensus CDS

CCDS48221.1

UniProtKB/Swiss-Prot

E9Q3T6, Q3URU1

UniProtKB/TrEMBL

A0A023UED4

Related

ENSMUSP00000044245.7, ENSMUST00000047577.7

Conserved Domains (5) summary

smart00317
Location:256 → 360

SET; SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain

COG5048
Location:449 → 545

COG5048; FOG: Zn-finger [General function prediction only]

sd00017
Location:453 → 473

ZF_C2H2; C2H2 Zn finger [structural motif]

pfam00096
Location:451 → 473

zf-C2H2; Zinc finger, C2H2 type

pfam13465
Location:494 → 518

zf-H2C2_2; Zinc-finger double domain