Rad52 RAD52 homolog, DNA repair protein [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Rad52provided by MGI
Official Full Name: RAD52 homolog, DNA repair proteinprovided by MGI
Primary source: MGI:MGI:101949
See related: Ensembl:ENSMUSG00000030166 AllianceGenome:MGI:101949
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Rad52yh
Summary: Predicted to enable identical protein binding activity and single-stranded DNA binding activity. Involved in DNA double-strand break processing involved in repair via single-strand annealing. Acts upstream of or within cellular response to oxidative stress and double-strand break repair via homologous recombination. Predicted to be part of protein-DNA complex. Predicted to be active in nucleus. Human ortholog(s) of this gene implicated in gastrointestinal system cancer (multiple); lung cancer (multiple); mucoepidermoid carcinoma; and nicotine dependence. Orthologous to human RAD52 (RAD52 homolog, DNA repair protein). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in thymus adult (RPKM 3.7), limb E14.5 (RPKM 3.3) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 6 F1; 6 56.86 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	6	NC_000072.7 (119878781..119899789)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	6	NC_000072.6 (119901722..119922827)

Chromosome 6 - NC_000072.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Rad52 mediates class-switch DNA recombination to IgD.
Title: Rad52 mediates class-switch DNA recombination to IgD.
RAD52 Adjusts Repair of Single-Strand Breaks via Reducing DNA-Damage-Promoted XRCC1/LIG3alpha Co-localization.
Title: RAD52 Adjusts Repair of Single-Strand Breaks via Reducing DNA-Damage-Promoted XRCC1/LIG3α Co-localization.
Suppression of Rad52 expression in combination with FANCM knockout drastically reduces cell and tumor growth, suggesting a synthetic lethality interaction between these two genes.
Title: The concerted roles of FANCM and Rad52 in the protection of common fragile sites.
Rad52 competes with Ku for binding to S-region double-strand DNA breaks (DSB) free ends, where it facilitates a DSB synaptic process, which favours intra-S region recombination.
Title: Rad52 competes with Ku70/Ku86 for binding to S-region DSB ends to modulate antibody class-switch DNA recombination.
Results demonstrate that Rad52 depletion increased cell death, decreased myeloid cell frequency, and augmented the activity of CD8+ T cells and NK effectors that ultimately led to reduced tumor growth. These data provide support for the notion of RAD52 as a potential oncogene, implicating a major role for the combined processes of recombinational repair and host immunity in determining risk for Squamous Cell.
Title: Rad52 deficiency decreases development of lung squamous cell carcinomas by enhancing immuno-surveillance.
In cancer-prone, heterozygous APC mutant mice, homozygous deletion of the Rad52 gene suppressed tumor growth and prolonged lifespan.
Title: Mammalian RAD52 Functions in Break-Induced Replication Repair of Collapsed DNA Replication Forks.
Rad54 has a key function in maintaining genomic integrity of the developing germ cells.
Title: Rad54 is required for the normal development of male and female germ cells and contributes to the maintainance of their genome integrity after genotoxic stress.
Results indicate that RAD52 cooperates with OGG1 to repair oxidative DNA damage and enhances the cellular resistance to oxidative stress.
Title: The recombination protein RAD52 cooperates with the excision repair protein OGG1 for the repair of oxidative lesions in mammalian cells.
RAD52 modulates the outcome of recombinant HIV-l vector infection by markedly reducing the efficiency of productive integration events
Title: Suppression of retroviral infection by the RAD52 DNA repair protein.
Loss of Rad52 partially rescues tumorigenesis and T-cell maturation in Atm-deficient mice.
Title: Loss of Rad52 partially rescues tumorigenesis and T-cell maturation in Atm-deficient mice.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (1) 1 citation

General gene information

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Markers

D11Bhm20 (e-PCR), detects polymorphism
- UniSTS:141619

D9Mit34.1 (e-PCR), detects polymorphism
- UniSTS:131903

Z32767 (e-PCR)
- UniSTS:163400

PMC109228P1 (e-PCR)
- UniSTS:270156

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	ISO Inferred from Sequence Orthology more info	PubMed
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables single-stranded DNA binding	IEA Inferred from Electronic Annotation more info
enables single-stranded DNA binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA damage response	ISO Inferred from Sequence Orthology more info	PubMed
involved_in DNA double-strand break processing involved in repair via single-strand annealing	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within DNA double-strand break processing involved in repair via single-strand annealing	ISO Inferred from Sequence Orthology more info	PubMed
involved_in DNA recombinase assembly	IEA Inferred from Electronic Annotation more info
involved_in DNA recombination	ISO Inferred from Sequence Orthology more info
involved_in DNA recombination	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within cellular response to oxidative stress	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cellular response to oxidative stress	ISO Inferred from Sequence Orthology more info	PubMed
involved_in double-strand break repair via homologous recombination	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within double-strand break repair via homologous recombination	IDA Inferred from Direct Assay more info	PubMed
involved_in double-strand break repair via single-strand annealing	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic recombination	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of nucleotide-excision repair	ISO Inferred from Sequence Orthology more info	PubMed

Component	Evidence Code	Pubs
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info	PubMed
part_of protein-DNA complex	IEA Inferred from Electronic Annotation more info
part_of protein-DNA complex	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info	PubMed

General protein information

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Preferred Names: DNA repair protein RAD52 homolog

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001166381.2 → NP_001159853.1 DNA repair protein RAD52 homolog isoform 1

See identical proteins and their annotated locations for NP_001159853.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longest isoform (1).

Source sequence(s)

AC117667

Consensus CDS

CCDS51887.1

UniProtKB/Swiss-Prot

P43352

Related

ENSMUSP00000125502.2, ENSMUST00000162461.7

Conserved Domains (1) summary

TIGR00607
Location:39 → 200

rad52; recombination protein rad52
NM_001166382.2 → NP_001159854.1 DNA repair protein RAD52 homolog isoform 3

Status: VALIDATED

Description

Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1.

Source sequence(s)

AC117667

Conserved Domains (1) summary

TIGR00607
Location:39 → 200

rad52; recombination protein rad52
NM_001410284.1 → NP_001397213.1 DNA repair protein RAD52 homolog isoform 5

Status: VALIDATED

Source sequence(s)

AC117667
NM_001410285.1 → NP_001397214.1 DNA repair protein RAD52 homolog isoform 6

Status: VALIDATED

Source sequence(s)

AC117667
NM_001410286.1 → NP_001397215.1 DNA repair protein RAD52 homolog isoform 7

Status: VALIDATED

Source sequence(s)

AC117667
NM_001410287.1 → NP_001397216.1 DNA repair protein RAD52 homolog isoform 8

Status: VALIDATED

Source sequence(s)

AC117667
NM_001410288.1 → NP_001397217.1 DNA repair protein RAD52 homolog isoform 9

Status: VALIDATED

Source sequence(s)

AC117667
NM_001410289.1 → NP_001397218.1 DNA repair protein RAD52 homolog isoform 10

Status: VALIDATED

Source sequence(s)

AC117667
NM_001410290.1 → NP_001397219.1 DNA repair protein RAD52 homolog isoform 11

Status: VALIDATED

Source sequence(s)

AC117667
NM_011236.3 → NP_035366.2 DNA repair protein RAD52 homolog isoform 4

See identical proteins and their annotated locations for NP_035366.2

Status: VALIDATED

Description

Transcript Variant: This variant (4) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 4), compared to isoform 1.

Source sequence(s)

AC117667

Consensus CDS

CCDS20477.1

UniProtKB/TrEMBL

G5E879

Related

ENSMUSP00000032269.7, ENSMUST00000032269.12

Conserved Domains (1) summary

TIGR00607
Location:39 → 200

rad52; recombination protein rad52

RNA

NR_149727.2 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (5) lacks an alternate internal exon, compared to variant 1.This variant is represented as non-coding because the use of the 5'-most expected translational start codon renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AC117667

Related

ENSMUST00000161045.8
NR_176974.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC117667
NR_176975.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC117667
NR_176977.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC117667
NR_176978.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC117667
NR_176979.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC117667
NR_176980.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC117667

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000072.7 Reference GRCm39 C57BL/6J

Range

119878781..119899789

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006505764.5 → XP_006505827.1 DNA repair protein RAD52 homolog isoform X1

See identical proteins and their annotated locations for XP_006505827.1

UniProtKB/TrEMBL

Q8VEE2

Conserved Domains (1) summary

TIGR00607
Location:39 → 200

rad52; recombination protein rad52
XM_006505765.5 → XP_006505828.1 DNA repair protein RAD52 homolog isoform X4

Conserved Domains (1) summary

cl01936
Location:1 → 144

Rad52_Rad22; Rad52/22 family double-strand break repair protein
XM_011241256.4 → XP_011239558.1 DNA repair protein RAD52 homolog isoform X2

Conserved Domains (1) summary

TIGR00607
Location:39 → 200

rad52; recombination protein rad52
XM_006505766.5 → XP_006505829.1 DNA repair protein RAD52 homolog isoform X5

Conserved Domains (1) summary

cl01936
Location:39 → 117

Rad52_Rad22; Rad52/22 family double-strand break repair protein
XM_036165959.1 → XP_036021852.1 DNA repair protein RAD52 homolog isoform X4

Conserved Domains (1) summary

cl01936
Location:1 → 144

Rad52_Rad22; Rad52/22 family double-strand break repair protein

RNA

XR_001785110.2 RNA Sequence
XR_001785111.2 RNA Sequence

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_001166383.1: Suppressed sequence

Description

NM_001166383.1: This RefSeq was removed because currently there is insufficient support for the 3'' end of the transcript and the C-terminus of the protein.
NR_176976.1: Suppressed sequence

Description

NR_176976.1: This RefSeq was removed because of redundancy with NR_176975.1.