Hoxb4 homeobox B4 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Hoxb4provided by MGI
Official Full Name: homeobox B4provided by MGI
Primary source: MGI:MGI:96185
See related: Ensembl:ENSMUSG00000038692 AllianceGenome:MGI:96185
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Hox-2.6
Summary: Enables DNA binding activity. Acts upstream of or within several processes, including embryonic skeletal system morphogenesis; hematopoietic or lymphoid organ development; and hemopoiesis. Predicted to be located in centrosome. Predicted to be active in nucleoplasm. Is expressed in several structures, including embryo mesenchyme; genitourinary system; gut; nervous system; and skin. Orthologous to human HOXB4 (homeobox B4). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in adrenal adult (RPKM 14.0), genital fat pad adult (RPKM 8.9) and 16 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 D; 11 59.83 cM

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (96209093..96212464)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (96318267..96321638)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Activated HoxB4-induced hematopoietic stem cells from murine pluripotent stem cells via long-term programming.
Title: Activated HoxB4-induced hematopoietic stem cells from murine pluripotent stem cells via long-term programming.
HOXB4 promoted the formation of hemogenic endothelium cells.
Title: HOXB4 Promotes Hemogenic Endothelium Formation without Perturbing Endothelial Cell Development.
Our data reveal a route to establishing functional multipotent HSCs that exploits aspects of in vitro differentiation, transcription factor re-specification with HoxB4 to enhance self-renewal, and Notch-mediated lineage specification
Title: Engineered Murine HSCs Reconstitute Multi-lineage Hematopoiesis and Adaptive Immunity.
can amplify long-term repopulating hematopoietic stem cells in a controlled way. This characteristic depends on a proline-rich sequence near the N terminus, which is unique among HOX genes and highly conserved in higher mammals
Title: Controlled stem cell amplification by HOXB4 depends on its unique proline-rich region near the N terminus.
Data suggest that the homeobox B4 protein (HoxB4) and signal transducer and activator of transcription 3 (STAT3) signals are functionally redundant for hematopoietic stem cells (HSCs) self-renewal rather than exerting independent effects.
Title: Molecular integration of HoxB4 and STAT3 for self-renewal of hematopoietic stem cells: a model of molecular convergence for stemness.
these studies define the transcriptional pathways involved in HOXB4 HSC expansion in vivo and identify repression of Prdm16 transcription as a mechanism by which expanding HSCs avoid leukemic transformation.
Title: Downregulation of Prdm16 mRNA is a specific antileukemic mechanism during HOXB4-mediated HSC expansion in vivo.
Hoxb4 favours the maintenance and increase of the CD4 central memory phenotype T cell population.
Title: Hoxb4 overexpression in CD4 memory phenotype T cells increases the central memory population upon homeostatic proliferation.
The results show a novel role for DNA methylation in the alternative promoter usage at the Runx1 locus and identify HOXB4 as a direct activator of the Runx1 P1 promoter.
Title: DNA methylation of Runx1 regulatory regions correlates with transition from primitive to definitive hematopoietic potential in vitro and in vivo.
Loss of Scmh1 caused derepression of Hoxb4 and Hoxa9, direct targets of Polycomb-group complex 1-mediated transcriptional silencing in hematopoietic cells.
Title: Scmh1 has E3 ubiquitin ligase activity for geminin and histone H2A and regulates geminin stability directly or indirectly via transcriptional repression of Hoxa9 and Hoxb4.
HOXB4 activation can generate a paracrine as well as a cell autonomous effect on hematopoietic differentiation.
Title: HOXB4 can enhance the differentiation of embryonic stem cells by modulating the hematopoietic niche.

Submit: New GeneRIF Correction See all GeneRIFs (34)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (9) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Hoxb4 (e-PCR), detects polymorphism
- UniSTS:536651

NoName (e-PCR)
- UniSTS:548175

G38056 (e-PCR)
- UniSTS:105044

Hoxb4 (e-PCR), detects polymorphism
- UniSTS:547240

PMC155209P2 (e-PCR)
- UniSTS:271331

PMC155209P3 (e-PCR)
- UniSTS:271332

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables sequence-specific double-stranded DNA binding	IEA Inferred from Electronic Annotation more info
enables sequence-specific double-stranded DNA binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in anterior/posterior pattern specification	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within anterior/posterior pattern specification	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within bone marrow development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within definitive hemopoiesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in embryonic skeletal system morphogenesis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within embryonic skeletal system morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in hematopoietic stem cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in hematopoietic stem cell differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within hematopoietic stem cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within hemopoiesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within morphogenesis of an epithelial sheet	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of stem cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of stem cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within skeletal system morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within skeletal system morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within somatic stem cell division	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within spleen development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in centrosome	IEA Inferred from Electronic Annotation more info
located_in centrosome	ISO Inferred from Sequence Orthology more info
is_active_in nucleoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	TAS Traceable Author Statement more info

General protein information

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Preferred Names: homeobox protein Hox-B4

Names: homeo box B4; homeobox protein Hox-2.6

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_010459.7 → NP_034589.3 homeobox protein Hox-B4

Status: VALIDATED

Source sequence(s)

AL606824

Consensus CDS

CCDS25297.1

UniProtKB/Swiss-Prot

P10284, Q3UZB4, Q4VBG0

Related

ENSMUSP00000048002.8, ENSMUST00000049241.9

Conserved Domains (1) summary

pfam00046
Location:164 → 218

Homeobox; Homeobox domain