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    aprA alkaline metalloproteinase [ Pseudomonas aeruginosa PAO1 ]

    Gene ID: 881248, updated on 10-Oct-2024

    Summary

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    Gene symbol
    aprA
    Gene description
    alkaline metalloproteinase
    Locus tag
    PA1249
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Pseudomonas aeruginosa PAO1 (strain: PAO1)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas
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    Genomic context

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    Sequence:
    NC_002516.2 (1355631..1357070)

    NC_002516.2Genomic Context describing neighboring genes Neighboring gene alkaline protease secretion ATP-binding protein AprD Neighboring gene alkaline protease secretion protein AprE Neighboring gene alkaline protease secretion protein AprF Neighboring gene alkaline proteinase inhibitor AprI Neighboring gene chemotaxis transducer

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_002516.2

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Alkaline protease contributes to pyocyanin production in Pseudomonas aeruginosa. Iiyama K, et al. FEMS Microbiol Lett, 2017 Apr 1. PMID 28333255
    2. Inducible polymerization and two-dimensional assembly of the repeats-in-toxin (RTX) domain from the Pseudomonas aeruginosa alkaline protease. Zhang L, et al. Biochemistry, 2014 Oct 21. PMID 25232897, Free PMC Article
    3. Activation of the epithelial sodium channel (ENaC) by the alkaline protease from Pseudomonas aeruginosa. Butterworth MB, et al. J Biol Chem, 2012 Sep 21. PMID 22859302, Free PMC Article
    4. Pseudomonas aeruginosa alkaline protease blocks complement activation via the classical and lectin pathways. Laarman AJ, et al. J Immunol, 2012 Jan 1. PMID 22131330
    5. Pseudomonas evades immune recognition of flagellin in both mammals and plants. Bardoel BW, et al. PLoS Pathog, 2011 Aug. PMID 21901099, Free PMC Article

    See all (19) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Molecular Analysis of the Contribution of Alkaline Protease A and Elastase B to the Virulence of Pseudomonas aeruginosa Bloodstream Infections.
      Title: Molecular Analysis of the Contribution of Alkaline Protease A and Elastase B to the Virulence of Pseudomonas aeruginosa Bloodstream Infections.
    2. AprA could contribute to pyocyanin production in the presence of several proteins or peptides
      Title: Alkaline protease contributes to pyocyanin production in Pseudomonas aeruginosa.
    3. Data suggest that polymerization of AprA into amyloid-like fibrils or large two-dimensional protein sheets occurs under near-physiological conditions, is rapid, involves the RTX domain, and can be controlled by regulating calcium in solution.
      Title: Inducible polymerization and two-dimensional assembly of the repeats-in-toxin (RTX) domain from the Pseudomonas aeruginosa alkaline protease.
    4. VIrulence factors AprA (alkaline proteasae) and Cif (CF transmembrane conductance regulator inhibitory factor) perturb sodium and chloride transport and osmosis in the lung.
      Title: Pouring salt on a wound: Pseudomonas aeruginosa virulence factors alter Na+ and Cl- flux in the lung.
    5. Structural and mutational studies reveal that the conserved N-terminal residues of AprI occupy the protease active site and are essential for AprA inhibitory activity.
      Title: Inhibition of Pseudomonas aeruginosa virulence: characterization of the AprA-AprI interface and species selectivity.
    6. AprA effectively degrades the TLR5 ligand monomeric flagellin and also prevents flagellin-mediated immune responses.
      Title: Pseudomonas evades immune recognition of flagellin in both mammals and plants.
    7. These data show that SP-D can contribute to the clearance of P. aeruginosa from the healthy ocular surface and that proteases can compromise that clearance.
      Title: Clearance of Pseudomonas aeruginosa from a healthy ocular surface involves surfactant protein D and is compromised by bacterial elastase in a murine null-infection model.
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    General gene information

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    Homology

    Gene Ontology Provided by PseudoCAP

    Function Evidence Code Pubs
    enables peptidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within negative regulation of complement activation, classical pathway IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within negative regulation of complement activation, lectin pathway IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of sodium ion transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    alkaline metalloproteinase
    NP_249940.1
    • Product name confidence: class 1 (Function experimentally demonstrated in P. aeruginosa)

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_002516.2 Reference assembly

      Range
      1355631..1357070
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_249940.1 alkaline metalloproteinase [Pseudomonas aeruginosa PAO1]

      See identical proteins and their annotated locations for NP_249940.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      K7Y5Z2, K7YJF7
      Conserved Domains (1) summary
      NF035945
      Location:13 → 479
      Zn_serralysin; serralysin family metalloprotease
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q03023.1

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