Mgat3 mannoside acetylglucosaminyltransferase 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mgat3provided by MGI
Official Full Name: mannoside acetylglucosaminyltransferase 3provided by MGI
Primary source: MGI:MGI:104532
See related: Ensembl:ENSMUSG00000042428 AllianceGenome:MGI:104532
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: GnT-III; 1110038J12Rik
Summary: Enables beta-1,4-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity. Acts upstream of or within several processes, including negative regulation of lysosomal protein catabolic process; positive regulation of protein localization to early endosome; and protein N-linked glycosylation. Predicted to be located in Golgi membrane and lysosome. Is expressed in several structures, including alimentary system; metanephros; and nervous system. Orthologous to human MGAT3 (beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Broad expression in colon adult (RPKM 15.5), kidney adult (RPKM 14.4) and 22 other tissues See more
Orthologs: human all
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Genomic context

Location:: 15 E1; 15 37.85 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	15	NC_000081.7 (80057852..80099720)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	15	NC_000081.6 (80173651..80215519)

Chromosome 15 - NC_000081.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

MiR-23a transcriptional activated by Runx2 increases metastatic potential of mouse hepatoma cell via directly targeting Mgat3
Title: MiR-23a transcriptional activated by Runx2 increases metastatic potential of mouse hepatoma cell via directly targeting Mgat3.
The MGAT3 and bisected complex N-glycans retard mouse mammary tumor progression; identification of key galectins that promote mammary tumor progression in mice is not straightforward because the galectin genes are expressed; and high levels of MGAT3.
Title: Bisected, complex N-glycans and galectins in mouse mammary tumor progression and human breast cancer.
data suggest that Fut8(-/-) mice adapted to oxidative stress, both ex vivo and in vivo, by inducing various genes including GnT-III, which may compensate for the loss of core fucose functions
Title: The absence of core fucose up-regulates GnT-III and Wnt target genes: a possible mechanism for an adaptive response in terms of glycan function.
Mgat3 glycogene expression and GnT-III-mediated glycosylation, specifically on E-cadherin, is a novel and major component of the Epithelial-Mesenchymal-Transition and the reverted process, Mesenchymal-Epithelial-Transition mechanism.
Title: Loss and recovery of Mgat3 and GnT-III Mediated E-cadherin N-glycosylation is a mechanism involved in epithelial-mesenchymal-epithelial transitions.
Roles of N-acetylglucosaminyltransferase III in epithelial-to-mesenchymal transition induced by transforming growth factor beta1 (TGF-beta1) in epithelial cell lines
Title: Roles of N-acetylglucosaminyltransferase III in epithelial-to-mesenchymal transition induced by transforming growth factor β1 (TGF-β1) in epithelial cell lines.
GnT-III expression may be precisely regulated by the interplay of E-cadherin-catenin complex-mediated cell-cell adhesion and Wnt/beta-catenin signaling
Title: Wnt/beta-catenin signaling down-regulates N-acetylglucosaminyltransferase III expression: the implications of two mutually exclusive pathways for regulation.
upregulation of GnT-III in AD brains may represent an adaptive response to protect them from additional beta-amyloid production
Title: Protective effect of N-glycan bisecting GlcNAc residues on beta-amyloid production in Alzheimer's disease.
beta1,4-N-acetylglucosaminyltransferase III inhibits the function of alpha5beta1 integrin
Title: Introduction of bisecting GlcNAc into integrin alpha5beta1 reduces ligand binding and down-regulates cell adhesion and cell migration.
mice homozygous for the Mgat3T37 mutation that express truncated GlcNAc-TIII from two mutant alleles suffer neurological and other consequences
Title: Truncated, inactive N-acetylglucosaminyltransferase III (GlcNAc-TIII) induces neurological and other traits absent in mice that lack GlcNAc-TIII.
An E-cadherin-dependent pathway plays a critical role in regulation of GnT-III expression; changes in GnT-III expression presumably contribute to intracellular signaling transduction during tissue development and homeostasis.
Title: Cell-cell interaction-dependent regulation of N-acetylglucosaminyltransferase III and the bisected N-glycans in GE11 epithelial cells. Involvement of E-cadherin-mediated cell adhesion.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables beta-1,4-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables beta-1,4-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity	ISO Inferred from Sequence Orthology more info
enables beta-1,4-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity	ISS Inferred from Sequence or Structural Similarity more info
enables glycosyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables glycosyltransferase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in N-acetylglucosamine metabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within N-acetylglucosamine metabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within amyloid-beta metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cellular response to oxidative stress	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cognition	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of lysosomal protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of lysosomal protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of protein localization to early endosome	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein N-linked glycosylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization	ISO Inferred from Sequence Orthology more info
involved_in protein localization	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of protein localization to early endosome	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell migration	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell migration	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in Golgi membrane	IEA Inferred from Electronic Annotation more info
located_in lysosome	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase

Names: N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III; glcNAc-T III

NP_034925.2

EC 2.4.1.144

XP_006520616.1

EC 2.4.1.144

XP_006520617.1

EC 2.4.1.144

XP_006520618.1

EC 2.4.1.144

XP_006520619.1

EC 2.4.1.144

XP_006520620.1

EC 2.4.1.144

XP_011243787.1

EC 2.4.1.144

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_010795.4 → NP_034925.2 beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase

See identical proteins and their annotated locations for NP_034925.2

Status: VALIDATED

Source sequence(s)

BC053040, CJ174488

Consensus CDS

CCDS27660.1

UniProtKB/Swiss-Prot

P70386, Q10470

UniProtKB/TrEMBL

Q5RKT9

Related

ENSMUSP00000043077.7, ENSMUST00000044970.7

Conserved Domains (1) summary

pfam04724
Location:205 → 502

Glyco_transf_17; Glycosyltransferase family 17

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000081.7 Reference GRCm39 C57BL/6J

Range

80057852..80099720

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011245485.2 → XP_011243787.1 beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase isoform X2

See identical proteins and their annotated locations for XP_011243787.1

UniProtKB/Swiss-Prot

P70386, Q10470

UniProtKB/TrEMBL

Q5RKT9

Conserved Domains (1) summary

pfam04724
Location:205 → 502

Glyco_transf_17; Glycosyltransferase family 17
XM_006520556.5 → XP_006520619.1 beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase isoform X2

See identical proteins and their annotated locations for XP_006520619.1

UniProtKB/Swiss-Prot

P70386, Q10470

UniProtKB/TrEMBL

Q5RKT9

Conserved Domains (1) summary

pfam04724
Location:205 → 502

Glyco_transf_17; Glycosyltransferase family 17
XM_006520555.2 → XP_006520618.1 beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase isoform X2

See identical proteins and their annotated locations for XP_006520618.1

UniProtKB/Swiss-Prot

P70386, Q10470

UniProtKB/TrEMBL

Q5RKT9

Conserved Domains (1) summary

pfam04724
Location:205 → 502

Glyco_transf_17; Glycosyltransferase family 17
XM_006520553.2 → XP_006520616.1 beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase isoform X1

UniProtKB/Swiss-Prot

P70386, Q10470

Conserved Domains (1) summary

pfam04724
Location:213 → 510

Glyco_transf_17; Glycosyltransferase family 17
XM_006520557.5 → XP_006520620.1 beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase isoform X2

See identical proteins and their annotated locations for XP_006520620.1

UniProtKB/Swiss-Prot

P70386, Q10470

UniProtKB/TrEMBL

Q5RKT9

Conserved Domains (1) summary

pfam04724
Location:205 → 502

Glyco_transf_17; Glycosyltransferase family 17
XM_006520554.5 → XP_006520617.1 beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase isoform X2

See identical proteins and their annotated locations for XP_006520617.1

UniProtKB/Swiss-Prot

P70386, Q10470

UniProtKB/TrEMBL

Q5RKT9

Conserved Domains (1) summary

pfam04724
Location:205 → 502

Glyco_transf_17; Glycosyltransferase family 17